公开(公告)号：US06261811B1

公开(公告)日：2001-07-17

申请号：US09468564

申请日：1999-12-21

Applicant: Mostafa K. Hamdy

Inventor： Mostafa K. Hamdy

IPC: C12P1706

CPC classification number: C12N11/02 ,  C12P17/06

Abstract: Natural product metabolites produced by fungi are synthesized using fungal spores that have been immobilized onto/into a support. Supports that can be used include loofah sponge, synthetic sponge, powdered cellulose paper, wood shavings, calcium alginate gel beads, agar gel beads, channeled aluminum beads, polypropylene beads and glass beads. Immobilizing the fungal spores provides accelerated production of the natural product metabolite in a standard bioreactor. Fungi that can be used include Penicillium cyclopium, Penicillium chrysogenum, Penicillium citrinum, Trichoderma viride, Aspergillus terreus and Monascus ruber. In a preferred embodiment, spores of Penicillium cyclopium NRRL 6233 immobilized with loofah sponge are cultured in a nutrient media for at least 4 days to produce compactin which is a hypocholesteremic agent.

Abstract translation: 由真菌产生的天然产物代谢物使用固定在载体上的真菌孢子合成。 可使用的支架包括丝瓜海绵，合成海绵，粉状纤维素纸，刨花，海藻酸钙凝胶珠，琼脂凝胶珠，通道铝珠，聚丙烯珠和玻璃珠。 固定真菌孢子可在标准生物反应器中加速生产天然产物代谢物。 可以使用的真菌包括Penicillium cyclopium，Penicillium chrysogenum，Penicillium citrinum，Trichoderma viride，Aspergillus terreus and Monascus ruber。 在优选的实施方案中，将固定有丝瓜海绵的青霉青霉NRRL 6233的孢子在营养培养基中培养至少4天以产生作为低胆固醇剂的致密蛋白。

公开(公告)号：US4929452A

公开(公告)日：1990-05-29

申请号：US370839

申请日：1989-06-22

Applicant: Mostafa K. Hamdy

Inventor： Mostafa K. Hamdy

IPC: C12C11/09 ,  C12G1/02 ,  C12M1/40

CPC classification number: C12C11/075 ,  C12C11/09 ,  C12G1/02 ,  C12M23/34 ,  C12M23/58 ,  C12M25/16

Abstract: A method for the accelerated brewing of alcohol and alcoholic beverages including beer, wine, and spirits utilizing flavor producing strains of S. cerevisiae and/or other bacteria such as lactobacillus spp. and a high alcohol-tolerant strain of S. cerevisiae. Preferred strains of S. cerevisiae for the production of beer are ATCC Nos. 20866 and 20867.In the preferred method, the fermentation is conducted in a bioreactor containing the flavor-producing strains and the high alcohol tolerant strain of S. cerevisiae immobilized on inert, high surface area supports, preferably porous organic or glass beads. The bioreactor significantly decreases the fermentation time necessary to produce a beverage containing a desired alcohol percentage. For example, beer can be processed in less than about 12 to 18 hours, wine in less than about 36 hours.

Abstract translation: 一种利用酿酒酵母和/或其它细菌如乳杆菌属的风味生产菌种加速酿造酒精和含酒精饮料的方法，包括啤酒，葡萄酒和烈酒。 和酿酒酵母的高耐酒菌株。 用于生产啤酒的酿酒酵母的优选菌株是ATCC20866和20867。在优选的方法中，发酵在含有产生风味的菌株和固定在惰性物质的酿酒酵母的高耐酒菌株的生物反应器中进行 ，高表面积的载体，优选多孔的有机或玻璃珠。 生物反应器显着降低生产含有所需醇百分比的饮料所需的发酵时间。 例如，啤酒可以在少于约12至18小时的时间内加工，葡萄酒不到36小时。