公开(公告)号：US5601993A

公开(公告)日：1997-02-11

申请号：US258483

申请日：1994-06-10

申请人： Motonari Kano ,  Masaru Matsumoto ,  Hiroshi Wada ,  Koki Motegi

发明人： Motonari Kano ,  Masaru Matsumoto ,  Hiroshi Wada ,  Koki Motegi

IPC分类号： A61K39/395 ,  C07K16/18 ,  G01N33/53 ,  G01N33/574 ,  G01N33/74 ,  G01N33/543

CPC分类号： C07K16/18 ,  G01N33/57419 ,  G01N33/743

摘要： Bile acids in the feces can be easily examined on a mass scale. Further, colon cancer at an early stage can be detected and the latent colon cancer can be diagnosed. The concentration of primary bile acid (CA) in the feces is measured by an ELISA method with the use of an anti-bile acid antibody for primary bile acid which comprises anti-cholic acid-24-bovine serum-rabbit serum, the concentration of secondary bile acid (DCA) in the feces is measured by an ELISA method with the use of an anti-bile acid antibody for secondary bile acid which comprises anti-deoxycholic acid-24-bovine serum-rabbit serum, and the concentration ratio (DCA/CA) of the secondary bile acid to the primary bile acid is calculated from the measured values.

摘要翻译： 粪便中的胆汁酸可以容易地以大规模检查。 此外，可以检测早期的结肠癌，并且可以诊断潜在的结肠癌。 通过使用包含抗胆酸24-牛血清 - 兔血清的原始胆汁酸的抗胆汁酸抗体的ELISA法测定粪便中原发性胆汁酸（CA）的浓度，浓度 通过使用包含抗脱氧胆酸-24-牛血清 - 兔血清的二级胆汁酸的抗胆汁酸抗体的ELISA法测定粪便中的次级胆汁酸（DCA），浓度比（DCA / CA）从初始胆汁酸计算。

公开(公告)号：US5631138A

公开(公告)日：1997-05-20

申请号：US381497

申请日：1995-01-31

申请人： Motonari Kano ,  Masaru Matsumoto

发明人： Motonari Kano ,  Masaru Matsumoto

IPC分类号： G01N33/53 ,  A61B10/00 ,  G01N33/50 ,  G01N33/531 ,  G01N33/543 ,  G01N33/576 ,  G01N33/92

CPC分类号： G01N33/92 ,  Y10S435/961 ,  Y10S435/973 ,  Y10S436/811 ,  Y10S436/822

摘要： A method for the measurement of serum bile acids by ELISA comprising: preparing a bile acid active ester, reacting the ester with a bovine serum albumin solution, dialyzing, and immunizing a mammal other than human being with the dialyzate thus obtained as an antigen to thereby give an anti-bile acid antibody; reacting said active ester with an enzyme to thereby prepare an enzyme-labeled bile acid as an enzyme-labeled antigen; to a secondary antibody-coated plate, adding a dilution of the serum to be assayed, an anti-bile acid antibody solution and an enzyme-labeled antigen solution and reacting these substances followed by the addition of a substrate and the reaction therewith; measuring the absorbance of the reaction mixture and determining the concentration of each bile acid on the basis of the standard curve measured simultaneously; and referring the sum of the concentrations of these bile acids to the total bile acid concentration; and a method for the diagnosis of a liver disease comprising: calculating the concentration ratio of each bile acid from the values measured by the former method; and comparing the concentration ratio thus obtained with the standard level scope of said concentration ratio obtained from healthy subjects are provided.

摘要翻译： 一种用于通过ELISA测量血清胆汁酸的方法，其包括：制备胆汁酸活性酯，使所述酯与牛血清白蛋白溶液反应，透析并用由此获得的透析液作为抗原免疫除人之外的哺乳动物，由此 给予抗胆汁酸抗体; 使所述活性酯与酶反应，从而制备酶标记的胆汁酸作为酶标记的抗原; 添加到待测细胞的稀释液，抗胆汁酸抗体溶液和酶标记的抗原溶液中，然后使这些物质反应，然后加入底物和与其反应; 测量反应混合物的吸光度，并根据同时测量的标准曲线测定每种胆汁酸的浓度; 并将这些胆汁酸的浓度之和表示为总胆汁酸浓度; 以及用于诊断肝脏疾病的方法，包括：从通过前一种方法测量的值计算每种胆汁酸的浓度比; 并比较由此获得的浓度比与从健康受试者获得的所述浓度比的标准水平范围。