公开(公告)号：US20140242673A1

公开(公告)日：2014-08-28

申请号：US14352320

申请日：2012-09-14

Applicant: NANJING UNIVERSITY OF TECHNOLOGY

Inventor： Min Jiang ,  Yongian Xi ,  Jiuhua Zhang ,  Rong Xu ,  Wenyu Dai ,  Kequan Chen ,  Ping Wei

IPC: C12N1/20

CPC classification number: C12N1/20 ,  C12P7/46

Abstract: This invention belongs to the technical field of industrial microorganism fermentation and relates to a type of chemical defined medium used for succinic acid production by fermentation and its application. The chemical defined medium of this invention used for succinic acid production by fermentation includes conventional compositions and critical growth factor compositions. Said critical growth factor compositions include biotin or 5-ALA, niacin, amino acid, and methionine. This formula determines four critical growth factors of succinic acid fermentation through reasonable technical means. The culture medium is free of complicated and expensive nutritious compositions such as yeast powder and peptone. Reliance on expensive vitamin by production of succinic acid through fermentation in defined medium is avoided, so that production cost of succinic acid can be greatly lowered, and subsequent separation process can be simplified, constituting a key step of industrial production of succinic acid using chemical defined medium.

Abstract translation: 本发明属于工业微生物发酵技术领域，涉及一种用于通过发酵生产琥珀酸的化学定义培养基及其应用。 用于通过发酵生产琥珀酸的本发明的化学定义的培养基包括常规组合物和临界生长因子组合物。 所述临界生长因子组合物包括生物素或5-ALA，烟酸，氨基酸和甲硫氨酸。 该公式通过合理的技术手段确定了琥珀酸发酵的四个关键生长因子。 培养基不含复杂且昂贵的营养成分如酵母粉和蛋白胨。 避免通过在定义的培养基中发酵生产琥珀酸来维持昂贵的维生素，从而大大降低了琥珀酸的生产成本，并且可以简化随后的分离过程，构成了使用化学定义的工业生产琥珀酸的关键步骤 中。