公开(公告)号：US20240091771A1

公开(公告)日：2024-03-21

申请号：US18246143

申请日：2021-09-17

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  NH FOODS LTD.

Inventor： Yusuke FUCHIWAKI ,  Masato TANAKA ,  Shohei YAMAMURA ,  Naoki MORISHITA ,  Kumiko KAMIYA ,  Seiichiro MATSUZAKI

IPC: B01L3/00

CPC classification number: B01L3/502723 ,  B01L2300/048 ,  B01L2300/069

Abstract: An assay device and an assay method are each capable of ensuring the accuracy of a target substance detection section. The assay device includes a plurality of assay units 100, each assay unit 100 including a microfluidic channel configured to allow a liquid to flow; a porous absorbing medium disposed at a distance from one end of the microfluidic channel, the one end being located on one side in a flow direction of the liquid; and a separation space disposed between the one end of the microfluidic channel and the porous absorbing medium, in which the microfluidic channel includes, in the microfluidic channel, a detection section 14 having immobilized thereon a substance capable of specifically reacting with a target substance, and an internal standard section 54 having immobilized thereon an internal standard substance, and each assay unit includes two parallel ventilation passages that are respectively adjacent to both sides of the microfluidic channel in the width direction orthogonal to the flow direction, the two parallel ventilation passages communicating with the microfluidic channel to allow for air circulation.

公开(公告)号：US20250135455A1

公开(公告)日：2025-05-01

申请号：US18683222

申请日：2022-07-27

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Yusuke FUCHIWAKI ,  Masato TANAKA ,  Shohei YAMAMURA

IPC: B01L3/00

Abstract: An assay device includes an inlet; an inner flow passage through which a liquid injected from the inlet flows; and a liquid absorbing material that absorbs the liquid that has passed through the inner flow passage. The inner flow passage include a microflow passage that includes an assay region and a separating flow passage that is provided between the microflow passage and the liquid absorbing material for separating the liquid therein when injection of the liquid is stopped, and the separating flow passage includes a narrowed width portion with a narrowed flow passage width.

公开(公告)号：US20240342705A1

公开(公告)日：2024-10-17

申请号：US18259645

申请日：2021-12-27

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  NH FOODS LTD.

Inventor： Yusuke FUCHIWAKI ,  Masato TANAKA ,  Shohei YAMAMURA ,  Naoki MORISHITA ,  Seiichiro MATSUZAKI

IPC: B01L3/00 ,  G01N35/08

CPC classification number: B01L3/502707 ,  G01N35/08 ,  B01L2300/048 ,  B01L2300/069 ,  B01L2300/0867

Abstract: An assay device allows reducing manufacturing variation, maintaining the measurement accuracy of the assay device at a high level, and enhancing the liquid control performance. The assay device of the present invention includes a microfluidic channel 2 allowing liquid to flow, an absorbing porous medium 3 disposed at a distance from one end 2a of the microfluidic channel 2, a separating space 4 disposed between the microfluidic channel 2 and the absorbing porous medium 3, and a housing space 5 housing the absorbing porous medium 3. The assay device further includes a lower member 20 which is an integrally molded article constituting a part of the assay device. The lower member 20 defines a lower portion 2b of the microfluidic channel 2, a lower portion 4a of the separating space 4, and a lower portion 5a of the housing space 5. The lower portions 4a, 5a of the separating space 4 and the housing space 5 are inclined downward toward one side from the other side in a flow direction. The lower member 20 supports the absorbing porous medium 3 at the lower portion 5a of the housing space 5.