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公开(公告)号：US20160138080A1
公开(公告)日：2016-05-19
申请号：US14775004
申请日：2014-03-13
Inventor： Ryoji Kurita , Hiroyuki Yanagisawa , Kyoko Yoshioka , Osamu Niwa
CPC classification number: C12Q1/683 , C12Q1/6804 , C12Q1/6827 , G01N33/5308 , G01N2440/12 , C12Q2537/164 , C12Q2521/307 , C12Q2563/131 , C12Q2565/501
Abstract: To provide a method for selectively detecting the methylation of particular cytosines in genomic DNA using a methylcytosine detection method using an anti-methylcytosine antibody to improve quantitativity and reliability. A method for detecting the methylated state of cytosine at a specific position contained in a nucleic acid, includes fragmenting the nucleic acid using a restriction enzyme; forming a double-stranded nucleic acid between the fragmented nucleic acid and a single-stranded nucleic acid having a base sequence capable of hybridizing with the fragmented nucleic acid but incapable of resulting in the formation of a base pair with cytosine at a specific position in the fragmented nucleic acid and a solid phase-binding site; binding the double-stranded nucleic acid on a solid phase using the solid phase-binding site; and measuring the amount of an antibody binding to the double-stranded nucleic acid on the solid phase.
Abstract translation: 提供一种使用抗甲基胞嘧啶抗体的甲基胞嘧啶检测方法选择性检测基因组DNA中特定胞嘧啶甲基化的方法，以提高定量性和可靠性。 用于检测核酸中包含的特定位置的胞嘧啶的甲基化状态的方法包括：使用限制酶片段化所述核酸; 在片段化的核酸和具有能够与片段化核酸杂交的碱基序列的单链核酸之间形成双链核酸，但不能导致与胞嘧啶在碱基对的特定位置形成碱基对 片段化核酸和固相结合位点; 使用固相结合位点在固相上结合双链核酸; 并测量在固相上与双链核酸结合的抗体的量。
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公开(公告)号：US09988672B2
公开(公告)日：2018-06-05
申请号：US14775004
申请日：2014-03-13
Inventor： Ryoji Kurita , Hiroyuki Yanagisawa , Kyoko Yoshioka , Osamu Niwa
IPC: C12Q1/6804 , C12Q1/68 , G01N33/53
CPC classification number: C12Q1/683 , C12Q1/6804 , C12Q1/6827 , G01N33/5308 , G01N2440/12 , C12Q2537/164 , C12Q2521/307 , C12Q2563/131 , C12Q2565/501
Abstract: To provide a method for selectively detecting the methylation of particular cytosines in genomic DNA using a methylcytosine detection method using an anti-methylcytosine antibody to improve quantitativity and reliability. A method for detecting the methylated state of cytosine at a specific position contained in a nucleic acid, includes fragmenting the nucleic acid using a restriction enzyme; forming a double-stranded nucleic acid between the fragmented nucleic acid and a single-stranded nucleic acid having a base sequence capable of hybridizing with the fragmented nucleic acid but incapable of resulting in the formation of a base pair with cytosine at a specific position in the fragmented nucleic acid and a solid phase-binding site; binding the double-stranded nucleic acid on a solid phase using the solid phase-binding site; and measuring the amount of an antibody binding to the double-stranded nucleic acid on the solid phase.
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