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公开(公告)号：US20180355413A1
公开(公告)日：2018-12-13
申请号：US15535906
申请日：2015-12-22
申请人： Anapa Biotech A/S
IPC分类号： C12Q1/6818
CPC分类号： C12Q1/6818 , C12Q2521/307 , C12Q2527/107 , C12Q2537/125 , C12Q2565/549 , C12Q2533/101
摘要： The present invention relates to a method for detecting at least one target nucleic acid sequence from a nucleic acid mixture by a double quenched assay. The double quenched assay of the method exploits a novel approach for melting temperature mediated identification of multiple target nucleic acid sequences. The invention further relates to a kit of parts.
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公开(公告)号：US20180353926A1
公开(公告)日：2018-12-13
申请号：US16010902
申请日：2018-06-18
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： B01J19/00 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
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公开(公告)号：US09999866B2
公开(公告)日：2018-06-19
申请号：US15287077
申请日：2016-10-06
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
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公开(公告)号：US09957558B2
公开(公告)日：2018-05-01
申请号：US13458739
申请日：2012-04-27
申请人： John Leamon , Mark Andersen , Michael Thornton
发明人： John Leamon , Mark Andersen , Michael Thornton
CPC分类号： C12Q1/686 , C12N15/1065 , C12Q1/6806 , C12Q1/6855 , C12Q1/6874 , C12Q1/6886 , C12Q2600/16 , C12Q2521/307 , C12Q2525/101 , C12Q2525/191 , C12Q2537/143
摘要： The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.
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公开(公告)号：US20180066313A1
公开(公告)日：2018-03-08
申请号：US15651017
申请日：2017-07-17
申请人： Gendiag.exe, S.L.
发明人： Eduardo Salas , José Manuel Soria , Miroslava Ogorelkova , Roberto Elosua Llanos , Joan Vila , Sergio Castillo Fernandez
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/6837 , C12Q2521/307 , C12Q2521/319 , C12Q2537/113 , C12Q2565/102 , C12Q2565/501 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/156
摘要： The invention relates to a method for a more appropriate thromboembolic event risk assessment based on the presence of different genetic variant. The invention also relates to a method for determining the risk of suffering a thromboembolism disease by combining the absence or presence of one or more polymorphic markers in a sample from the subject with conventional risk factors for thromboembolism as well as computer-implemented means for carrying out said method.
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公开(公告)号：US20180016628A1
公开(公告)日：2018-01-18
申请号：US15426930
申请日：2017-02-07
CPC分类号： C12Q1/6837 , C12N15/1093 , C12Q1/682 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151 , C12Q2521/313 , C12Q2565/513 , C12Q2521/307
摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
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公开(公告)号：US09809844B2
公开(公告)日：2017-11-07
申请号：US13107400
申请日：2011-05-13
申请人： David C. Schwartz , Kyubong Jo , Dalia M. Dhingra
发明人： David C. Schwartz , Kyubong Jo , Dalia M. Dhingra
CPC分类号： C12Q1/6837 , B01L3/5027 , B82Y15/00 , B82Y30/00 , C12Q1/6816 , C12Q1/6869 , Y10S436/80 , C12Q2537/143 , C12Q2565/631 , C12Q2527/137 , C12Q2521/307 , C12Q2565/601
摘要： Methods are provided for tagging, characterizing and sorting double-stranded biomolecules while maintaining the integrity of the biomolecules.
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公开(公告)号：US20170152554A1
公开(公告)日：2017-06-01
申请号：US15425791
申请日：2017-02-06
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.
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公开(公告)号：US20170021325A1
公开(公告)日：2017-01-26
申请号：US15287077
申请日：2016-10-06
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US20160298184A1
公开(公告)日：2016-10-13
申请号：US15184469
申请日：2016-06-16
申请人： Affymetrix, Inc.
发明人： Michael H. Shapero
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6809 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6876 , C12Q2600/156 , C12Q2565/514 , C12Q2563/149 , C12Q2521/301 , C12Q2525/155 , C12Q2563/131 , C12Q2565/537 , C12Q2537/143 , C12Q2531/113 , C12Q2565/501 , C12Q2521/307
摘要： The present invention provides for methods for discriminating between alleles at polymorphic positions in a genome. In general the methods employ allele specific extension of oligonucleotides that are complementary to one of the alleles at the 3′ end of the oligonucleotide. The allele specific oligonucleotides are resistant to proof reading activity from a polymerase and may be extended in an allele specific manner by a DNA polymerase with a functional 3′ to 5′ exonuclease activity. The allele specific oligonucleotides may be attached to a solid support such as a chip or a bead.
摘要翻译： 本发明提供了用于区分基因组中多态性位置处的等位基因的方法。 通常，该方法采用与寡核苷酸3'末端的等位基因之一互补的寡核苷酸的等位基因特异性延伸。 等位基因特异性寡核苷酸对聚合酶的抗读取活性具有抗性，并且可以通过具有功能3'至5'核酸外切酶活性的DNA聚合酶以等位基因特异性方式延伸。 等位基因特异性寡核苷酸可以连接到固体支持物如芯片或珠粒上。
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