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1.发明申请公开(公告)号：US20190169584A1
公开(公告)日：2019-06-06
申请号：US16124127
申请日：2018-09-06
申请人： The Regents of the University of California , National Technology & Engineering Solutions of Sandia, LLC , Pacific Northwest National Laboratory (PNNL)
发明人： John M. Gladden , Saori Amaike Campen , Jinxiang Zhang , Jon K. Magnuson , Scott E. Baker , Blake A. Simmons
摘要： The present invention provides for an Asperigillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Asperigillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
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2.发明授权公开(公告)号：US11384367B2
公开(公告)日：2022-07-12
申请号：US16450824
申请日：2019-06-24
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC , BATTELLE MEMORIAL INSTITUTE (FOR MANAGEMENT AND OPERATION OF PACIFIC NORTHWEST NATIONAL LABORATORY)
摘要： The present invention provides for a method of converting a depolymerized lignin aromatic compound into a bioproduct, comprising: (a) providing a composition comprising a depolymerized lignin aromatic compound, optionally a depolymerized cellulose, and optionally a depolymerized hemicellulose, and (b) introducing a genetically modified microorganism to the composition, wherein the genetically modified microorganism is capable of converting the depolymerized lignin aromatic compound into a bioproduct; such that the depolymerized lignin aromatic compound is converted into a bioproduct.
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公开(公告)号：US20190136281A1
公开(公告)日：2019-05-09
申请号：US16308440
申请日：2017-06-07
发明人： John M. Gladden , Tanmoy Dutta , Jian Sun , Seema Singh , Blake A. Simmons
摘要： Disclosed herein are methods for preparing sugar compositions. The methods include: i) forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and the ionic liquid contains a) a cation and b) a sugar acid anion or a ketoacid anion; ii) maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; iii) adding at least one glycoside hydrolase to the mixture; and iv) maintaining the mixture containing the glycoside hydrolase under conditions sufficient to hydrolyze at least a portion of the dissolved polysaccharide, thereby forming the sugar compositions. The sugar compositions contain at least one monosaccharide or oligosaccharide. New sugar-based ionic liquids are also described.
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公开(公告)号：US20210198708A1
公开(公告)日：2021-07-01
申请号：US17190050
申请日：2021-03-02
发明人： John M. Gladden , Tanmoy Dutta , Jian Sun , Seema Singh , Blake A. Simmons
摘要： Disclosed herein are methods for preparing sugar compositions. The methods include: i) forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and the ionic liquid contains a) a cation and b) a sugar acid anion or a ketoacid anion; ii) maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; iii) adding at least one glycoside hydrolase to the mixture; and iv) maintaining the mixture containing the glycoside hydrolase under conditions sufficient to hydrolyze at least a portion of the dissolved polysaccharide, thereby forming the sugar compositions. The sugar compositions contain at least one monosaccharide or oligosaccharide. New sugar-based ionic liquids are also described.
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公开(公告)号：US10934568B2
公开(公告)日：2021-03-02
申请号：US16308440
申请日：2017-06-07
发明人： John M. Gladden , Tanmoy Dutta , Jian Sun , Seema Singh , Blake A. Simmons
摘要： Disclosed herein are methods for preparing sugar compositions. The methods include: i) forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and the ionic liquid contains a) a cation and b) a sugar acid anion or a ketoacid anion; ii) maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; iii) adding at least one glycoside hydrolase to the mixture; and iv) maintaining the mixture containing the glycoside hydrolase under conditions sufficient to hydrolyze at least a portion of the dissolved polysaccharide, thereby forming the sugar compositions. The sugar compositions contain at least one monosaccharide or oligosaccharide. New sugar-based ionic liquids are also described.
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6.发明授权公开(公告)号：US11414653B2
公开(公告)日：2022-08-16
申请号：US16124127
申请日：2018-09-06
申请人： The Regents of the University of California , National Technology & Engineering Solutions of Sandia, LLC , Pacific Northwest National Laboratory (PNNL)
发明人： John M. Gladden , Saori Amaike Campen , Jinxiang Zhang , Jon K. Magnuson , Scott E. Baker , Blake A. Simmons
摘要： The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
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公开(公告)号：US10941388B2
公开(公告)日：2021-03-09
申请号：US15985216
申请日：2018-05-21
申请人： The Regents of the University of California , National Technology & Engineering Solutions of Sandia, LLC
摘要： The present invention provides for a purified or isolated cellulase complex comprising two or more glycosidase hydrolase, or enzymatically active fragment thereof, selected from the group consisting of a GH9 polypeptide, a GH48 polypeptide, a GH10 polypeptide, and a GH6 polypeptide, and optionally a GH10_2 polypeptide and/or an AA10 polypeptide.
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8.发明申请公开(公告)号：US20180371443A1
公开(公告)日：2018-12-27
申请号：US15985216
申请日：2018-05-21
申请人： The Regents of the University of California , National Technology & Engineering Solutions of Sandia, LLC
摘要： The present invention provides for a purified or isolated cellulase complex comprising two or more glycosidase hydrolase, or enzymatically active fragment thereof, selected from the group consisting of a GH9 polypeptide, a GH48 polypeptide, a GH10 polypeptide, and a GH6 polypeptide, and optionally a GH10_2 polypeptide and/or an AA10 polypeptide.
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