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    PROTEIN PRODUCTION METHOD USING TRANSFORMED PLANT CELLS
    2.
    发明申请
    PROTEIN PRODUCTION METHOD USING TRANSFORMED PLANT CELLS 审中-公开
    使用变形植物细胞的蛋白质生产方法

    公开(公告)号：US20140315248A1

    公开(公告)日：2014-10-23

    申请号：US14240581

    申请日：2012-08-29

    Applicant: NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Ko Kato ,  Kiyotaka Ueda ,  Renya Okawara ,  Toshihiro Yamura

    IPC: C12P21/00

    CPC classification number: C12P21/00 ,  C12N15/8216 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8257 ,  C12P21/02

    Abstract: An object of this invention is to produce a protein by efficiently culturing plant cells while escaping mRNA translational repression in cultured plant cells under stress caused by lack of conditions essentially required for the growth (e.g., nutrient-starvation stress and hypoxic stress). This invention provides a method for escaping translational repression of a protein encoded by mRNA, the repression being induced by stress due to absence of conditions required for the growth, the method comprising the step of culturing a plant cell transformed with a recombinant DNA molecule encoding mRNA containing 5′ UTR defined in (a) or (b) below, the culturing being carried out under stress due to absence of conditions required for the growth: (a) 5′ UTR having a base sequence of SEQ ID NO: 1, 2, 3, 4, 5, or 6; or (b) 5′ UTR having the base sequence of the 5′ UTR of (a) in which one or more bases are replaced, deleted, or added, and which escapes translational repression induced by stress due to absence of conditions required for the growth.

    Abstract translation: 本发明的一个目的是通过有效培养植物细胞，同时避免在缺乏基本上生长所需的条件（例如营养饥饿压力和缺氧胁迫）的胁迫下在培养的植物细胞中的mRNA翻译抑制。 本发明提供了一种逃避由mRNA编码的蛋白质的翻译抑制的方法，该抑制是由于不存在生长所需的条件而被胁迫诱导的，该方法包括培养用编码mRNA的重组DNA分子转化的植物细胞的步骤 在下文（a）或（b）中定义的含有5'UTR的培养是由于不需要生长条件而在胁迫下进行的：（a）具有SEQ ID NO：1,2的碱基序列的5'UTR ，3,4,5或6; 或（b）具有（a）的5'UTR的碱基序列的5'UTR，其中一个或多个碱基被替换，缺失或添加，并且由于不存在所需的条件而导致由胁迫引起的翻译抑制 成长。

    DNA MOLECULE CODING FOR 5' UTR ENABLING HIGH RECOMBINANT PROTEIN EXPRESSION IN MONOCOTYLEDONS
    3.
    发明申请
    DNA MOLECULE CODING FOR 5' UTR ENABLING HIGH RECOMBINANT PROTEIN EXPRESSION IN MONOCOTYLEDONS 有权

    公开(公告)号：US20210171966A1

    公开(公告)日：2021-06-10

    申请号：US17047226

    申请日：2019-04-09

    Applicant: National University Corporation Nara Institute of Science and Technology

    Inventor： Ko Kato

    IPC: C12N15/82

    Abstract: The purpose of the present invention is to provide technology for identifying a 5′ UTR having excellent translation ability while taking into account 5′ UTR variants in monocotyledons, providing a DNA molecule that codes for the 5′ UTR, and efficiently producing a recombinant protein in the monocotyledons. It is possible to efficiently produce a recombinant protein in a monocotyledon by using a nucleic acid structure in a polynucleotide that codes for a foreign protein, said structure comprising a base sequence among the base sequences shown in sequences 1-4 and linking to a 5′ UTR or a variant thereof.

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