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    CPMV enhancer elements
    2.
    发明授权
    CPMV enhancer elements 有权

    公开(公告)号：US11441150B2

    公开(公告)日：2022-09-13

    申请号：US15110696

    申请日：2015-01-08

    申请人： Medicago Inc.

    发明人： Pierre-Olivier Lavoie ,  Marc-Andre D'Aoust

    IPC分类号： C12N15/82 ,  C12N7/04

    摘要： An expression enhancer comprising a CPMV 5′UTR nucleotide sequence consisting of X nucleotides (CMPVX), where X=160, 155, 150, or 114 of SEQ ID NO:1, or consisting of a nucleotide sequence comprising from about 80% to 100% sequence similarity with CMPVX, where X=160, 155, 150, or 114 of SEQ ID NO:1 SEQ ID NO:1 is provided. The expression enhancer may further comprise a stuffer sequence fused to the 3′ end of the 5′UTR nucleotide sequence (CMPVX+, where X=160, 155, 150, or 114 of SEQ ID NO:1). The stuffer sequence may comprise one or more plant kozak sequences. Plants comprising the expression enhancer and methods using the expression enhancer are also described.

    Increasing virus-like particle yield in plants
    3.
    发明授权
    Increasing virus-like particle yield in plants 有权

    公开(公告)号：US11155581B2

    公开(公告)日：2021-10-26

    申请号：US14347804

    申请日：2012-09-28

    申请人： MEDICAGO INC.

    发明人： Marc-Andre D'aoust ,  Manon Couture ,  Louis-Philippe Vezina

    IPC分类号： C07K14/005 ,  C12N15/82 ,  A61K39/145 ,  C12N7/00 ,  C07K16/10

    摘要： A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a structural virus protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.

    Modified CPMV enhancer elements
    6.
    发明授权
    Modified CPMV enhancer elements 有权

    公开(公告)号：US10563213B2

    公开(公告)日：2020-02-18

    申请号：US15129269

    申请日：2015-03-27

    申请人： Medicago Inc.

    发明人： Marc-Andre D'Aoust ,  Pierre-Olivier Lavoie

    IPC分类号： C12N15/82 ,  C07K14/11

    摘要： An expression enhancer comprising, in series, a CPMV 5′UTR nucleotide sequence comprising nucleotides 1-160 of SEQ ID NO:1, or comprising a nucleotide sequence comprising from about with 80% to 100% sequence similarity with SEQ ID NO:1, and a stuffer fragment is provided. The stuffer fragment comprises a nucleotide sequence encoding an incomplete M protein and one or more kozak sequence active in a plant. Plants and plant matter comprising the expression enhancer and methods using the expression enhancer are also described.

    Rotavirus-like particle production in plants
    7.
    发明授权
    Rotavirus-like particle production in plants 有权

    公开(公告)号：US10287555B2

    公开(公告)日：2019-05-14

    申请号：US15545362

    申请日：2016-01-21

    申请人： MEDICAGO, INC. ,  MITSUBISHI TANABE PHARMA CORPORATION

    发明人： Pierre-Olivier Lavoie ,  Marc-Andre D'Aoust

    IPC分类号： C12N7/04 ,  A61K39/12 ,  C12N7/02 ,  C12N15/82 ,  C12N15/86 ,  A61K39/00

    摘要： A method of producing a rotavirus-like particle (RLP) in a plant is provided. The method comprises expressing within a host or host cell for example a plant, portion of a plant or plant cell one or more nucleic acid comprising one or more regulatory region operatively linked to a first, second and third nucleotide sequence, the regulatory region active in the host or host cell. The first nucleotide sequence encoding a first rotavirus protein, the second nucleotide sequence encoding a second rotavirus protein and the third nucleotide sequence encoding a third rotavirus protein. The first, second and third encode rotavirus protein NSP4 and VP2 or VP6 and VP4 or VP7. The host or host cell is incubated under conditions that permit the expression of the nucleic acids, so that NSP4 and either VP2 of VP6 and VP4 or VP7 are expressed, thereby producing the RLP. Hosts comprising the RLP, compositions comprising the RLP and method for using the composition are also provided.