公开(公告)号：US20230279361A1

公开(公告)日：2023-09-07

申请号：US18006920

申请日：2021-07-27

Applicant: NATIONAL UNIVERSITY CORPORATION SHIGA UNIVERSITY OF MEDICAL SCIENCE

Inventor： Yasushi ITOH ,  Hirohito ISHIGAKI ,  Misako NAKAYAMA ,  Yoshinori KITAGAWA

IPC: C12N7/00 ,  C12N5/071 ,  C07K16/10

CPC classification number: C12N7/00 ,  C12N5/0686 ,  C07K16/10 ,  C12N2501/734 ,  C12N2770/20032 ,  C12N2770/20051

Abstract: The present invention addresses the problem of providing a more convenient method for in vitro proliferating a virus belonging to the family Coronaviridae at high proliferation accuracy. This problem is solved by an in vitro virus proliferation method, said method comprising a step for culturing kidney-derived cells, which have been contacted with the virus belonging to the family Coronaviridae, with a use of a cell culture medium to which a protease having an optimum pH of from 7 to 9 is added.