公开(公告)号：US09631164B2

公开(公告)日：2017-04-25

申请号：US14770006

申请日：2014-03-19

Applicant: Novozymes A/S

Inventor： Marc Dominique Morant ,  Anna Verana Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

IPC: C12N9/20 ,  C12Q1/34 ,  C12Q1/44 ,  C07H21/04 ,  C11D3/00 ,  C11D7/44 ,  C12S11/00 ,  C11D3/386 ,  C12P13/00

CPC classification number: C11D3/38627 ,  C11D3/38681 ,  C12N9/20 ,  C12P13/008

Abstract: The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3 or the full-length complement of (i); (c) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3; (d) a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and (e) a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

公开(公告)号：US20160010038A1

公开(公告)日：2016-01-14

申请号：US14770006

申请日：2014-03-19

Applicant: NOVOZYMES A/S

Inventor： Marc Dominique Morant ,  Anna Verana Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

IPC: C11D3/386 ,  C12P13/00 ,  C12N9/20

CPC classification number: C11D3/38627 ,  C11D3/38681 ,  C12N9/20 ,  C12P13/008

Abstract: The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3 or the full-length complement of (i); (c) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3; (d) a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and (e) a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

Abstract translation: 本发明涉及具有脂肪酶活性的分离的多肽，其选自：（a）多肽，其具有至少75％，至少80％，至少85％，至少90％，至少91％，在 与SEQ ID NO：4的至少92％，至少93％，至少94％，至少95％，至少96％，至少97％，至少98％，至少99％或100％的序列同一性 ; （b）由多核苷酸编码的多肽，其在低严格条件，中等严格条件，中等严格条件，高严格条件或非常高严格条件下与（i）SEQ ID NO：3或全长补体 的（i）; （c）由多核苷酸编码的多肽，所述多核苷酸具有至少75％，至少80％，至少85％，至少90％，至少91％，至少92％，至少93％，至少94％ 与SEQ ID NO：3的至少95％，至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （d）在一个或多个（例如数个）位置上包含取代，缺失和/或插入的SEQ ID NO：4的变体的多肽; 和（e）作为（a），（b），（c）或（d）的多肽的片段的多肽。 本发明还涉及编码多肽的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。