公开(公告)号：US09512420B2

公开(公告)日：2016-12-06

申请号：US14396317

申请日：2013-11-27

Applicant: Nanjing University of Technology

Inventor： Jingjing Xie ,  Wenyan Ji ,  Hanjie Ying ,  Wujin Sun ,  Ting Guo ,  Yong Chen ,  Xiaochun Chen ,  Jinglan Wu

IPC: C12N9/00 ,  C12N9/88 ,  C12P19/26 ,  C12N15/63

CPC classification number: C12N9/88 ,  C12N15/63 ,  C12P19/26 ,  C12Y401/03003 ,  Y02P20/52

Abstract: It discloses a use of N-acetylneuraminic acid aldolase with an amino acid sequence as shown in SEQ ID NO: 2 in catalytic synthesis of N-acetylneuraminic acid. The preparation of N-acetylneuraminic acid is to use the N-acetylneuraminic acid aldolase with the amino acid sequence as shown in SEQ ID NO: 2 as a catalyst, and N-acetylmannosamine and pyruvic acid as substrates.

Abstract translation: 其公开了N-乙酰神经氨酸醛缩酶与N-乙酰神经氨酸的催化合成中具有SEQ ID NO：2所示氨基酸序列的用途。 N-乙酰神经氨酸的制备是使用N-乙酰神经氨酸醛缩酶与SEQ ID NO：2所示的氨基酸序列作为催化剂，N-乙酰甘露糖胺和丙酮酸作为底物。

公开(公告)号：US10144925B2

公开(公告)日：2018-12-04

申请号：US14651071

申请日：2012-12-13

Applicant: NANJING UNIVERSITY OF TECHNOLOGY

Inventor： Hanjie Ying ,  Yong Chen ,  Qingguo Liu ,  Jinglan Wu ,  Xiaochun Chen ,  Jingjing Xie

IPC: C12N11/08 ,  C12N11/12 ,  C12P7/06 ,  C12N11/02 ,  D06M13/123 ,  D06M13/332 ,  D06M13/418 ,  D06M15/59

Abstract: Provided is a preparation method of a yeast cell immobilization medium, which comprises the following steps: (1) boiling a fiber material in boiling water and drying the fiber material; (2) soaking the fiber material in a surface modified aqueous solution with a concentration of 1-100 g/L, using hydrochloric acid to adjust a PH of the solution to 7.0, fully rinsing the fiber material in deionized water and drying the fiber material; (3) soaking the fiber material in a cross-linking agent aqueous solution with a concentration of 1-100 g/L, fully rinsing the fiber material in deionized water and drying the fiber material; and (4) attaching the fiber material to supporting framework. Also provided is the yeast cell immobilization medium prepared using the preparation method and a method for producing ethanol using the yeast cell immobilization medium.

公开(公告)号：US20160017308A1

公开(公告)日：2016-01-21

申请号：US14396317

申请日：2013-11-27

Applicant: NANJING UNIVERSITY OF TECHNOLOGY

Inventor： Jingjing Xie ,  Wenyan Ji ,  Hanjie Ying ,  Wujin Sun ,  Ting Guo ,  Yong Chen ,  Xiaochun Chen ,  Jinglan Wu

IPC: C12N9/88

CPC classification number: C12N9/88 ,  C12N15/63 ,  C12P19/26 ,  C12Y401/03003 ,  Y02P20/52

Abstract: It discloses a use of N-acetylneuraminic acid aldolase with an amino acid sequence as shown in SEQ ID NO: 2 in catalytic synthesis of N-acetylneuraminic acid. The preparation of N-acetylneuraminic acid is to use the N-acetylneuraminic acid aldolase with the amino acid sequence as shown in SEQ ID NO: 2 as a catalyst, and N-acetylmannosamine and pyruvic acid as substrates.

Abstract translation: 其公开了N-乙酰神经氨酸醛缩酶与N-乙酰神经氨酸的催化合成中具有SEQ ID NO：2所示氨基酸序列的用途。 N-乙酰神经氨酸的制备是使用N-乙酰神经氨酸醛缩酶与SEQ ID NO：2所示的氨基酸序列作为催化剂，N-乙酰甘露糖胺和丙酮酸作为底物。