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公开(公告)号：US20080194508A1
公开(公告)日：2008-08-14
申请号：US11884247
申请日：2006-02-06
CPC分类号： G01N15/1459 , G01N33/543 , G01N33/54366 , G01N33/54393 , G01N2015/1486 , Y10T436/101666
摘要： The inventions relates to compositions and method for determining the absolute counts of cells per unit volume of a sample. Such a method comprises: (a) providing a container containing: (i) a predetermined quantity of microparticles; and (ii) a cell-binding agent; in which the microparticles are disposed in or on a matrix which adheres to at least one wall of the container such that substantially all the microparticles are thereby attached to the container; (b) adding a known volume of sample to the container; (c) determining the ratio of microparticles to cells by counting microparticles and cells in a volume of the sample; and (d) determining the absolute count of cells by multiplying the number of cells per microparticle by the concentration of microparticles in the sample. Preferably, the matrix retains substantially all the microparticles in or on the container during routine handling, including inversion, of the container, in the absence of mechanical retaining means such as a retainer grid in the container.
摘要翻译： 本发明涉及用于确定样品每单位体积的细胞的绝对计数的组合物和方法。 这种方法包括：（a）提供容器，其包含：（i）预定量的微粒; 和（ii）细胞结合剂; 其中微粒设置在基质中或基体上，该基质附着在容器的至少一个壁上，使得基本上所有的微粒从而附着到容器上; （b）向容器中加入已知体积的样品; （c）通过计数样品体积中的微粒和细胞来确定微粒与细胞的比例; 和（d）通过将每个微粒的细胞数乘以样品中微粒的浓度来确定细胞的绝对计数。 优选地，在没有诸如容器中的保持架网格的机械保持装置的情况下，基体在基本上将容器内的所有微粒保持在容器的常规处理（包括反转）中。
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公开(公告)号：US08465944B2
公开(公告)日：2013-06-18
申请号：US12526323
申请日：2008-02-11
申请人： Nanna K. Christensen , Lars Winther
发明人： Nanna K. Christensen , Lars Winther
摘要： The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.
摘要翻译： 本发明涉及一种增强固定在固定介质中的组织或细胞样品的免疫反应性的方法，目标检索组合物及其用途。 该方法包括在水平位置提供载体，所述载体上具有组织或细胞样品，所述组织或细胞样品位于载体的顶部; 基本上使载体的组织或细胞样品侧与缓冲的目标检索溶液接触，其中目标检索溶液仍然暴露于环境中; 将组织或细胞样品和目标回收溶液加热至高于100℃的温度。本发明还涉及所述样品的自动化免疫组织化学染色，特别是通过固定掩蔽的抗原的再活化。
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公开(公告)号：US20100136612A1
公开(公告)日：2010-06-03
申请号：US12526323
申请日：2008-02-11
申请人： Nanna K. Christensen , Lars Winther
发明人： Nanna K. Christensen , Lars Winther
IPC分类号： G01N1/30
摘要： The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.
摘要翻译： 本发明涉及一种增强固定在固定介质中的组织或细胞样品的免疫反应性的方法，目标检索组合物及其用途。 该方法包括在水平位置提供载体，所述载体上具有组织或细胞样品，所述组织或细胞样品位于载体的顶部; 基本上使载体的组织或细胞样品侧与缓冲的目标检索溶液接触，其中目标检索溶液仍然暴露于环境中; 将组织或细胞样品和目标回收溶液加热至高于100℃的温度。本发明还涉及所述样品的自动化免疫组织化学染色，特别是通过固定掩蔽的抗原的再活化。
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