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    Horizontal antigen retrieval
    2.
    发明授权
    Horizontal antigen retrieval 有权
    水平抗原检索

    公开(公告)号：US08465944B2

    公开(公告)日：2013-06-18

    申请号：US12526323

    申请日：2008-02-11

    申请人： Nanna K. Christensen ,  Lars Winther

    发明人： Nanna K. Christensen ,  Lars Winther

    IPC分类号： G01N1/28 ,  G01N1/44 ,  G01N1/30 ,  G01N33/48

    CPC分类号： G01N33/50 ,  G01N1/30

    摘要： The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.

    摘要翻译： 本发明涉及一种增强固定在固定介质中的组织或细胞样品的免疫反应性的方法，目标检索组合物及其用途。 该方法包括在水平位置提供载体，所述载体上具有组织或细胞样品，所述组织或细胞样品位于载体的顶部; 基本上使载体的组织或细胞样品侧与缓冲的目标检索溶液接触，其中目标检索溶液仍然暴露于环境中; 将组织或细胞样品和目标回收溶液加热至高于100℃的温度。本发明还涉及所述样品的自动化免疫组织化学染色，特别是通过固定掩蔽的抗原的再活化。

    Method of pre-treatment and staining of a biological sample and device for support of a biological sample and methods of using such device
    3.
    发明授权
    Method of pre-treatment and staining of a biological sample and device for support of a biological sample and methods of using such device 有权
    用于支持生物样品的生物样品和装置的预处理和染色方法以及使用这种装置的方法

    公开(公告)号：US08283176B2

    公开(公告)日：2012-10-09

    申请号：US11168987

    申请日：2005-06-28

    申请人： Loren L. Bland ,  Marc E. Key ,  Lars Winther ,  David A. Stanforth ,  Martin N. Lindberg

    发明人： Loren L. Bland ,  Marc E. Key ,  Lars Winther ,  David A. Stanforth ,  Martin N. Lindberg

    IPC分类号： G01N33/00

    CPC分类号： G01N1/30 ,  B01L3/545 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0822 ,  G01N1/312 ,  G01N35/00732 ,  G01N2035/00782 ,  Y10T436/255

    摘要： There is disclosed a method of pre-treatment and staining, according to a protocol, of a biological sample disposed upon the surface of a carrier, the method comprising the step of recording at least one parameter relating to at least one protocol step in a non-volatile memory located either upon or within the carrier or a device incorporating the carrier. Also disclosed is a device comprising: a non-volatile memory; a surface of the device adapted to carry a biological sample; and communications means electrically coupled to the memory for enabling data transmission to or from an external apparatus. Also disclosed is a method of controlling processing of a biological sample disposed upon a carrier, comprising: providing, upon or within the carrier or an apparatus holding the carrier, a non-volatile memory having information relating to sample processing priority or protocol; reading the information; and scheduling the processing based upon the information.

    摘要翻译： 公开了根据方案对设置在载体表面上的生物样品进行预处理和染色的方法，所述方法包括以下步骤：将与至少一个方案步骤相关的至少一个参数记录在非 - 位于载体上或载体内的易失性存储器或包含载体的装置。 还公开了一种装置，包括：非易失性存储器; 所述装置的表面适于携带生物样品; 以及电连接到存储器的通信装置，用于使能到外部设备的数据传输。 还公开了一种控制设置在载体上的生物样品的处理的方法，包括：在载体或保持载体的装置的上方或内部提供具有与样品处理优先级或方案有关的信息的非易失性存储器; 阅读信息; 并基于该信息调度处理。

    METHOD FOR PURIFICATION OF TARGET POLYPEPTIDES
    5.
    发明申请
    METHOD FOR PURIFICATION OF TARGET POLYPEPTIDES 审中-公开
    目标多肽的纯化方法

    公开(公告)号：US20120071633A1

    公开(公告)日：2012-03-22

    申请号：US13319261

    申请日：2010-05-04

    申请人： Jan Khyse-Andersen ,  Lars Winther

    发明人： Jan Khyse-Andersen ,  Lars Winther

    IPC分类号： C07K16/18 ,  C07K19/00

    CPC分类号： C07K1/22 ,  C07K1/32 ,  C07K1/36

    摘要： The present invention relates to a process for purification of a target molecule, comprising the steps: (a) contacting a target molecule, and a population of target binding polypeptides (TBP), in solution for a sufficient time to allow complex formation; and (b) isolating the target from the complex from (a) by subsequent purification steps, wherein (i) the target binding polypeptides have at least two binding functionalities; a first binding functionality towards the target and a second binding functionality towards a catching ligand comprised in a solid support; and (ii) the first binding functionality comprises at least two binding sites for the target, and the target comprises at least two binding sites for the TBP.

    摘要翻译： 本发明涉及一种纯化靶分子的方法，包括以下步骤：（a）在溶液中使目标分子和靶结合多肽（TBP）群体接触足够的时间以允许复合物形成; 和（b）通过随后的纯化步骤从（a）分离所述复合物中的靶，其中（i）靶结合多肽具有至少两个结合官能团; 针对靶的第一结合功能和朝向包含在固体支持物中的捕获性配体的第二结合功能; 和（ii）第一结合功能包含靶的至少两个结合位点，并且靶包含TBP的至少两个结合位点。

    On-Site Liquid Production
    7.
    发明申请
    On-Site Liquid Production 审中-公开
    现场液体生产

    公开(公告)号：US20110045498A1

    公开(公告)日：2011-02-24

    申请号：US12675232

    申请日：2008-08-25

    申请人： Martin Lindberg ,  Lars Winther

    发明人： Martin Lindberg ,  Lars Winther

    IPC分类号： G01N33/567 ,  C12M1/02 ,  C12M1/36

    CPC分类号： G01N35/00029 ,  G01N2035/00138 ,  G01N2035/00217 ,  G01N2035/00544

    摘要： A biological sample processing system for on-site liquid production comprises a processing apparatus for processing of biological samples arranged on microscope slides, and a production unit connected to the apparatus. The production unit comprises a first ingredient source, a second ingredient source, and a mixer station. The mixer station is configured to mix ingredients to produce a liquid product. Supply conduits are arranged to supply an amount of a first ingredient and an amount of a second ingredient from the first and second ingredient sources to the mixer station. A delivery conduit is provided for transportation of an amount of the liquid product from the mixer station. A production controller is arranged in communication with the production unit, and is configured to control the operation of the production unit.

    摘要翻译： 用于现场液体生产的生物样品处理系统包括用于处理布置在显微镜载片上的生物样品的处理装置和连接到该装置的生产单元。 生产单元包括第一成分源，第二成分源和混合站。 搅拌站配置成混合成分以产生液体产品。 供应管道被布置成将一定量的第一成分和第二成分的量从第一和第二成分源提供给混合器站。 输送管道用于运输来自混合站的液体产品的量。 生产控制器被布置成与生产单元通信，并且被配置为控制生产单元的操作。

    Systems and Methods for Biological and Chemical Detection, Comprising Automatic Selection of Reagent Sets
    8.
    发明申请
    Systems and Methods for Biological and Chemical Detection, Comprising Automatic Selection of Reagent Sets 审中-公开
    包括试剂盒自动选择的生物和化学检测系统和方法

    公开(公告)号：US20100151460A1

    公开(公告)日：2010-06-17

    申请号：US12516678

    申请日：2007-11-29

    申请人： Lars Winther

    发明人： Lars Winther

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34 ,  G06F19/00

    CPC分类号： G01N33/6803 ,  G01N33/58

    摘要： The instant invention relates to systems of reagents and methods for the detection of chemical and biological targets in a sample. Some embodiments comprise methods for automatically selecting a set of reagents to detect one or more targets in a sample, wherein the set of reagents comprises at least two layers for detection of a first target, and at least two layers for detection of a second target, wherein the set comprises reagents that are at least partially redundant. In some embodiments, the redundancy is created by at least one degenerate reagent such that the reagent may interact specifically with more than one other component of a detection system or sample. In some embodiments, the system or method also includes reagent containers with a computer-generated code which may further serve to match targets to appropriate reagents.

    摘要翻译： 本发明涉及用于检测样品中化学和生物学靶标的试剂和方法的系统。 一些实施方案包括用于自动选择一组试剂以检测样品中的一种或多种靶的方法，其中该组试剂包含用于检测第一靶的至少两层和用于检测第二靶的至少两层， 其中该组包含至少部分冗余的试剂。 在一些实施方案中，冗余由至少一种简并试剂产生，使得试剂可以与检测系统或样品的多于一种其它成分特异性地相互作用。 在一些实施方案中，所述系统或方法还包括具有计算机生成的代码的试剂容器，其可进一步用于将靶标与适合的试剂相匹配。

    Standard for immunohistochemistry, immunocytochemistry and molecular cytogenetics
    9.
    发明申请
    Standard for immunohistochemistry, immunocytochemistry and molecular cytogenetics 审中-公开
    免疫组织化学，免疫细胞化学和分子细胞遗传学标准

    公开(公告)号：US20070037138A1

    公开(公告)日：2007-02-15

    申请号：US10547033

    申请日：2004-02-27

    申请人： Lars Winther

    发明人： Lars Winther

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  G01N33/53 ,  C12M1/34

    CPC分类号： G01N33/96 ,  C12Q1/6841 ,  G01N1/06 ,  G01N1/36 ,  G01N15/1468 ,  G01N2001/2893 ,  G01N2015/1472

    摘要： This application describes a method comprising: (a) providing a reference standard or a planar section thereof, the reference standard comprising (i) a support medium; and (ii) a quantity of a detectable entity supported by the support medium; in which the detectable entity has an elongate path; in which a predetermined amount of the detectable entity is present in a defined region in a cross section of the referenced standard; (b) obtaining a first reference signal indicative of the presence or quantity of detectable entity in the reference standard, planar section thereof, or the defined region; (c) providing a biological sample and obtaining a second signal indicative of the presence or quantity of detectable entity in the biological sample, or a component thereof; and (d) comparing the reference signal obtained in (b) against the second signal obtained in (c). Preferably, the method is used to indicate the presence, quantity or concentration of a detectable entity in a biological sample.

    摘要翻译： 本申请描述了一种方法，包括：（a）提供参考标准或其平面部分，所述参考标准包括（i）支撑介质; 和（ii）支持媒介支持的可检测实体的数量; 其中可检测实体具有细长路径; 其中预定量的可检测实体存在于参考标准的横截面中的限定区域中; （b）获得指示参考标准，其平面部分或限定区域中可检测实体的存在或数量的第一参考信号; （c）提供生物样品并获得指示生物样品或其组分中可检测实体的存在或数量的第二信号; 和（d）将（b）中获得的参考信号与（c）中获得的第二信号进行比较。 优选地，该方法用于指示生物样品中可检测实体的存在，数量或浓度。