公开(公告)号：US20080038757A1

公开(公告)日：2008-02-14

申请号：US11596385

申请日：2005-05-13

申请人： Naoko Shibayama ,  Keishi Miwa

发明人： Naoko Shibayama ,  Keishi Miwa

IPC分类号： G01N33/535 ,  C12M1/40

CPC分类号： G01N33/54393 ,  G01N33/543 ,  G01N33/6869 ,  G01N2333/5412

摘要： A measurement kit capable of rapidly and conveniently detecting one type or a plurality of types of target substance(s) simultaneously in a sample and a measurement method therefor are provided. Furthermore, with the kit or the measurement method, disease with which a target substance is associated is diagnosed. The enzyme immunological kit comprises: a support upon the surface of which a substance that recognizes a target substance is immobilized; an enzyme-labeled antibody against the target substance; and a substrate whose reaction product is water-insoluble after the enzyme-labeled antibody is caused to undergo color development; with which the target substance in a sample is detected based on color development on the support surface. Alternatively, the measurement method comprises 2 reaction steps: the incubation step and the color development step, by which a target substance in a sample is detected or the concentration of the target substance in the sample is measured through visual determination of color developed on the support surface after the color development reaction.

摘要翻译： 本发明提供能够快速方便地检测样品中的一种或多种目标物质同时检测的测定试剂及其测定方法。 此外，通过试剂盒或测量方法，诊断出与其相关的目标物质的疾病。 酶免疫试剂盒包括：其表面上的支持物，其识别目标物质的物质被固定; 针对靶物质的酶标记抗体; 以及使酶标记的抗体发生显色后，其反应产物为水不溶性的底物; 基于载体表面上的颜色显现来检测样品中的目标物质。 或者，测量方法包括2个反应步骤：孵育步骤和显色步骤，通过该步骤检测样品中的目标物质或样品中目标物质的浓度，通过目视确定在载体上显影的颜色 表面后发色反应。