公开(公告)号：US20190185913A1

公开(公告)日：2019-06-20

申请号：US16309271

申请日：2017-06-30

Applicant: Natera, Inc.

Inventor： Bernhard ZIMMERMANN ,  Joshua BABIARZ ,  Raheleh SALARI ,  Tudor Pompiliu CONSTANTIN ,  Onur SAKARYA ,  Dennis PROSEN ,  Alexander OLSON ,  Scott DASHNER ,  Nikolay SERGEEV ,  Matthew Micah HILL

IPC: C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6886

CPC classification number: C12Q1/686 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16 ,  C40B40/06 ,  G01N2800/7028 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2537/143

Abstract: The invention provides methods and compositions for detecting a mutation in a target gene in a sample of blood or a fraction thereof, including in certain examples, a fraction that includes circulating tumor DNA. The methods can include a tiling PCR reaction, for example a one-sided multiplex tiling reaction. Virtually any type of mutation can be detected with the methods and compositions. In certain embodiments, gene fusions are detected. Improved PCR methods, especially for performing nested multiplex PCR reactions are provided.