公开(公告)号：US11091804B2

公开(公告)日：2021-08-17

申请号：US16752339

申请日：2020-01-24

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/68 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q1/6811 ,  C12Q1/6848

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US10351906B2

公开(公告)日：2019-07-16

申请号：US15336630

申请日：2016-10-27

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/68 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q1/6811 ,  C12Q1/6848

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20190256894A1

公开(公告)日：2019-08-22

申请号：US16400023

申请日：2019-04-30

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/686 ,  C12Q1/6848 ,  C12Q1/6811 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q1/68

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20170145474A1

公开(公告)日：2017-05-25

申请号：US15336630

申请日：2016-10-27

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2537/143

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US09677118B2

公开(公告)日：2017-06-13

申请号：US14538982

申请日：2014-11-24

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2537/143

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US10597708B2

公开(公告)日：2020-03-24

申请号：US16400023

申请日：2019-04-30

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/68 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q1/6811 ,  C12Q1/6848

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US10597709B2

公开(公告)日：2020-03-24

申请号：US16412301

申请日：2019-05-14

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/68 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q1/6811 ,  C12Q1/6848

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20150322507A1

公开(公告)日：2015-11-12

申请号：US14538982

申请日：2014-11-24

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Matthew Hill ,  Philippe Lacroute ,  Michael Dodd ,  Alexander Wong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2537/143

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

摘要翻译： 本发明提供了在一个反应​​体积中同时扩增多个核酸区域的方法以及用于选择用于这种扩增方法的引物文库的方法。 本发明还提供具有所需特征的引物文库，例如扩增引物二聚体或其他非靶扩增子的最小形成。