公开(公告)号：US20220154249A1

公开(公告)日：2022-05-19

申请号：US17600789

申请日：2020-04-14

Applicant: Natera, Inc.

Inventor： Bernhard ZIMMERMANN ,  Ryan SWENERTON ,  Fei LU ,  James STRAY ,  Jason TONG

IPC: C12Q1/6806 ,  C12Q1/686

Abstract: Provided herein are improved methods of determining the sequences of cell-free DNA (cfDNA). The methods in certain embodiments are used for the analysis of circulating DNA in serum samples, such as circulating fetal DNA, circulating donor derived DNA, or circulating tumor DNA. In certain embodiments, the methods include selectively enriching trinucleosomal, dinucleosomal, mononucleosomal or sub-mononucleosomal DNA from the isolated cfDNA.

公开(公告)号：US20210009990A1

公开(公告)日：2021-01-14

申请号：US16969892

申请日：2019-02-15

Applicant: Natera, Inc.

Inventor： James STRAY ,  Jason TONG

IPC: C12N15/10 ,  C12Q1/6853

Abstract: Disclosed here is a method for isolating nucleic acids from a biological sample, comprising: (a) contacting a first composition comprising nucleic acids obtained from a biological sample with a first matrix under a low-stringency binding condition having less than 1% aliphatic alcohols that binds less than 5% of nucleic acids of shorter than about 118 bp and more than 30% of nucleic acids longer than about 194 bp to a first matrix; and (b) contacting a second composition comprising remainder of the first composition with a second matrix under a high-stringency binding condition having less than 1% aliphatic alcohol that binds more than 70% of nucleic acids longer than about 72 bp and 30% of nucleic acids longer than about 50 bp to the second matrix. Further disclosed is a kit for isolating nucleic acids from a biological sample, comprising (a) a first binding buffer for establishing a low-stringency binding condition having less than 1% aliphatic alcohols that binds less than 5% of nucleic acids shorter than about 118 bp and more than 30% of nucleic acids longer than about 194 bp to a matrix, and (b) a second binding buffer for establishing a high-stringency binding condition having less than 1% aliphatic alcohol that binds more than 70% of nucleic acids longer than about 72 bp and 30% of nucleic acids longer than about 50 bp to the matrix.

公开(公告)号：US20180237841A1

公开(公告)日：2018-08-23

申请号：US15898080

申请日：2018-02-15

Applicant: Natera, Inc.

Inventor： James STRAY ,  Jason TONG ,  Xiaoke WANG

IPC: C12Q1/6834

CPC classification number: C12Q1/6834 ,  C12N15/1006

Abstract: Disclosed here is a composition for isolating nucleic acids from a biological sample, comprising a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof. Also disclosed is a method for binding nucleic acids to a matrix, comprising: contacting the nucleic acids from a biological sample with the matrix in the presence of a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof, thereby binding the nucleic acids to the matrix. Further disclosed is a kit for isolating nucleic acids from a biological sample comprising a binding buffer, wherein the binding buffer comprises a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof.