公开(公告)号：US20230203573A1

公开(公告)日：2023-06-29

申请号：US17925693

申请日：2021-05-27

Applicant: Natera, Inc.

Inventor： Ryan SWENERTON ,  Bernhard ZIMMERMANN ,  Ebad AHMED ,  Nathan LIANG ,  Allison RYAN ,  Fei LU ,  Paul VAN HUMMELEN

IPC: C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6876

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2600/158

Abstract: The present disclosure provides methods for quantifying the amount of total cell-free DNA in a biological sample, comprising: isolating cell-free DNA from the biological sample, wherein a first Tracer DNA composition is added before or after isolation of the cell-free DNA; performing targeted amplification at 100 or more different target loci in a single reaction volume using 100 or more different primer pairs; sequencing the amplification products by high-throughput sequencing to generate sequencing reads; and quantifying the amount of total cell-free DNA using sequencing reads derived from the first Tracer DNA composition.

公开(公告)号：US20220154249A1

公开(公告)日：2022-05-19

申请号：US17600789

申请日：2020-04-14

Applicant: Natera, Inc.

Inventor： Bernhard ZIMMERMANN ,  Ryan SWENERTON ,  Fei LU ,  James STRAY ,  Jason TONG

IPC: C12Q1/6806 ,  C12Q1/686

Abstract: Provided herein are improved methods of determining the sequences of cell-free DNA (cfDNA). The methods in certain embodiments are used for the analysis of circulating DNA in serum samples, such as circulating fetal DNA, circulating donor derived DNA, or circulating tumor DNA. In certain embodiments, the methods include selectively enriching trinucleosomal, dinucleosomal, mononucleosomal or sub-mononucleosomal DNA from the isolated cfDNA.

公开(公告)号：US20210071246A1

公开(公告)日：2021-03-11

申请号：US16959949

申请日：2019-01-11

Applicant: Natera, Inc.

Inventor： Bernhard ZIMMERMANN ,  Ryan SWENERTON ,  Scott DASHNER ,  Fei LU ,  Himanshu SETHI

IPC: C12Q1/6853 ,  C12Q1/6858

Abstract: Disclosed here is a composition comprising a primer that is (a) a loopable primer comprising a target-specific section, an adaptor section, and a stem-forming section, wherein the stem-forming section is hybridizable to a portion of the target-specific section to form a stem structure, or (b) a split primer comprising a first target-specific section, a second target-specific section, and an adaptor section positioned between the first target-specific section and the second target-specific section, or (c) a split-loopable primer comprising a first target-specific section, a second target-specific section, a stem-forming section positioned between the first target-specific section and the second target-specific section, and an adaptor section, or comprising a first adaptor section, a second adaptor section, a stem-forming section positioned between the first adaptor section and the second adaptor section, and a target-specific section. Also disclosed is a method for amplifying a target locus of interest from a template DNA, comprising at least two pre-amplification cycles using the loopable primer, the split primer and/or the split-loopable primer, wherein each amplification cycle comprises annealing the primer to the template DNA or pre-amplification product thereof and elongating the annealed primer. Further disclosed is a kit for amplifying a target locus of interest, comprising the loopable primer, the split primer, and/or the split-loopable primer.