公开(公告)号：US20230204600A1

公开(公告)日：2023-06-29

申请号：US18009632

申请日：2021-06-11

申请人： National Institute of Advanced Industrial Science and Technology

发明人： Tomoko WATANABE ,  Hiroaki TATENO ,  Shuuji MAWARIBUCHI ,  Yoshikazu HARAMOTO

IPC分类号： G01N33/68

CPC分类号： G01N33/6887 ,  G01N2470/04

摘要： An object of the present invention is to provide a novel method that enables determination or evaluation of the undifferentiated state of human pluripotent stem cells simply, efficiently and non-invasively. The present invention provides a method for determining or evaluating the undifferentiated state of human pluripotent stem cells, comprising a step of detecting or measuring fibronectin in a culture supernatant of human pluripotent stem cells.

公开(公告)号：US20210322513A1

公开(公告)日：2021-10-21

申请号：US17364162

申请日：2021-06-30

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  UNIVERSITY OF TSUKUBA

发明人： Hiroaki TATENO ,  Jun HIRABAYASHI ,  Makoto ASASHIMA ,  Yuzuru ITOU ,  Yasuko ONUMA ,  Tatsuya ODA ,  Nobuhiro OHKOHCHI ,  Osamu SHIMOMURA

IPC分类号： A61K38/17 ,  G01N33/574 ,  C12Q1/02 ,  G01N33/53 ,  A61K35/74 ,  A61K47/42 ,  A61P35/00 ,  A61K38/16

摘要： As a technique for specifically detecting cancer cells, provided is a method for detecting cancer cells, including the steps of: bringing BC2LCN lectin into contact with a test sample; and determining the presence or absence or the amount of a sugar chain having a BC2LCN lectin binding activity in the test sample, in which the test sample is a body fluid sample of a test individual.

公开(公告)号：US20180250360A1

公开(公告)日：2018-09-06

申请号：US15759288

申请日：2016-10-05

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  UNIVERSITY OF TSUKUBA

发明人： Hiroaki TATENO ,  Jun HIRABAYASHI ,  Makoto ASASHIMA ,  Yuzuru ITOU ,  Yasuko ONUMA ,  Tatsuya ODA ,  Nobuhiro OHKOHCHI ,  Osamu SHIMOMURA

IPC分类号： A61K38/17 ,  A61K38/16 ,  A61P35/00 ,  G01N33/574

CPC分类号： A61K38/178 ,  A61K35/74 ,  A61K38/164 ,  A61K47/42 ,  A61P35/00 ,  C12Q1/02 ,  G01N33/53 ,  G01N33/574 ,  G01N2333/7056

摘要： As a technique for specifically detecting cancer cells, provided is a method for detecting cancer cells, including the steps of: bringing BC2LCN lectin into contact with a test sample; and determining the presence or absence or the amount of a sugar chain having a BC2LCN lectin binding activity in the test sample, in which the test sample is a body fluid sample of a test individual.

公开(公告)号：US20230117360A1

公开(公告)日：2023-04-20

申请号：US17913698

申请日：2021-03-15

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： Hiroaki TATENO

IPC分类号： G01N33/53 ,  C12Q1/686 ,  G01N33/58

摘要： A method for analyzing a cell surface glycan is provided, the method including bringing a glycan-binding substance labeled with a nucleic acid into contact with the cell and detecting the nucleic acid labeled to the glycan-binding substance bound to the cell, in which a kind and a quantity of the nucleic acid correspond to a kind and a quantity of the cell surface glycan.

公开(公告)号：US20220236275A1

公开(公告)日：2022-07-28

申请号：US17717764

申请日：2022-04-11

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  UNIVERSITY OF TSUKUBA

发明人： Hiroaki TATENO ,  Tatsuya ODA ,  Osamu SHIMOMURA

IPC分类号： G01N33/574 ,  G01N33/548

摘要： The present invention relates to a method for analyzing a biological sample obtained from a subject, comprising analyzing components contained in a biological sample by an assay using a first molecule selected from the group consisting of a molecule binding to a sugar chain bindable with BC2LCN, a molecule binding to SerpinA3, and a molecule binding to Gal3BP, and a second molecule that is BC2LCN.

公开(公告)号：US20180038847A1

公开(公告)日：2018-02-08

申请号：US15548921

申请日：2015-12-21

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  WAKO PURE CHEMICAL INDUSTRIES, LTD.

发明人： Hiroaki TATENO ,  Masaki WARASHINA ,  Masakazu FUKUDA ,  Kazunari HIRAYASU

IPC分类号： G01N33/50 ,  C07H5/04 ,  G01N33/574

摘要： Provided is a method for detecting a stem cell based on an undifferentiated sugar chain marker having a specific sugar chain structure, wherein the stem cell is detected by detecting podocalyxin contained in a culture supernatant or a lysate of cells by a “lectin-antibody sandwich method” using a combination of a lectin and an antibody and having high sensitivity, the method including steps of: contacting the culture supernatant or the lysate, a lectin capable of binding to a sugar chain represented by (Formula 1) or (Formula 2), and an antibody capable of binding to keratan sulfate to form a complex composed of the lectin, podocalyxin and the antibody; and detecting the complex. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule.