公开(公告)号：US20030222222A1

公开(公告)日：2003-12-04

申请号：US10160251

申请日：2002-06-04

Applicant: National Taiwan University

Inventor： Chen-Yuan Dong ,  Peter T.C. So ,  Sunney I. Chan

IPC: G01N021/64

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/0446 ,  G01N2201/0453

Abstract: This invention provides a multiphoton excitaion microscope for simultaneously detecting differently colored fluorescence materials on biochips, which includes a multiphoton excitation source, objectives, and a plurality of detection channels. The biochip is hybridized and labeled with fluorescence materials for expressing hybridized biological signals. The multiphoton excitation source is focused to a light spot on the biochip to excite the fluorescence materials bound thereon. After that, the fluorescence emission at different wavelengths from the different fluorescent materials can be detected by the plural detection channels.

Abstract translation: 本发明提供了一种多光子激发显微镜，用于同时检测生物芯片上的不同颜色的荧光材料，其包括多光子激发源，物镜和多个检测通道。 将生物芯片杂交并用用于表达杂交的生物信号的荧光材料标记。 多光子激发源被聚焦到生物芯片上的光点以激发结合在其上的荧光材料。 之后，可以通过多个检测通道检测来自不同荧光材料的不同波长的荧光发射。