公开(公告)号：US20220372445A1

公开(公告)日：2022-11-24

申请号：US17497409

申请日：2021-10-08

申请人： National Yang Ming Chiao Tung University

发明人： Guan-Yu Chen ,  Jia-Wei Yang

IPC分类号： C12N5/071

摘要： Disclosed herein is a method for cultivating primary human pulmonary alveolar epithelial cells (HPAEpiC), which includes cultivating the primary HPAEpiC in a first medium containing a basal medium, a culture supplement, and a Rho kinase inhibitor, and a second medium containing the basal medium and the culture supplement in sequence. The culture supplement includes Jagged-1 (JAG-1) peptide, human Noggin protein, transforming growth factor-β (TGF-β) type I receptor inhibitor SB431542, human fibroblast growth factor 7 (hFGF-7), hFGF-10, and glycogen synthase kinase 3 (GSK-3) inhibitor CHIR99021. Also disclosed is a method for preparing a three-dimensional cell culture of alveolar epithelium using the first medium and the second medium.

公开(公告)号：US20240037745A1

公开(公告)日：2024-02-01

申请号：US18172706

申请日：2023-02-22

申请人： National Yang Ming Chiao Tung University

发明人： Guan-Yu Chen ,  Ren-Hao Xie ,  Shiue-Luen Chen

IPC分类号： G06T7/00 ,  G06V10/28 ,  G06V10/25 ,  G06V10/44

CPC分类号： G06T7/0014 ,  G06V10/28 ,  G06V10/25 ,  G06V10/457 ,  G06T2207/10016 ,  G06T2207/30061 ,  G06V2201/07

摘要： A method of tracking movement of particles in bronchus includes obtaining, from a video of the particles, a plurality of processed images, each of which contains a plurality of particle portions representing the particles, and implementing a tracking process for a reference image of the processed images. The tracking process includes, for each particle portion in the reference image, selecting a to-be-compared portion from among the particle portions in a next image immediately following the reference image, obtaining an expected range of movement of the particle represented by the particle portion according to a position of the particle portion, determining whether a corresponding position in the reference image that corresponds to a position of the to-be-compared portion in the next image is within the expected range, and storing the position of the to-be-compared portion into a track record that is associated with the particle when the determination is affirmative.

公开(公告)号：US11795437B2

公开(公告)日：2023-10-24

申请号：US17497409

申请日：2021-10-08

申请人： National Yang Ming Chiao Tung University

发明人： Guan-Yu Chen ,  Jia-Wei Yang

IPC分类号： C12N5/071

CPC分类号： C12N5/0688 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54

摘要： Disclosed herein is a method for cultivating primary human pulmonary alveolar epithelial cells (HPAEpiC), which includes cultivating the primary HPAEpiC in a first medium containing a basal medium, a culture supplement, and a Rho kinase inhibitor, and a second medium containing the basal medium and the culture supplement in sequence. The culture supplement includes Jagged-1 (JAG-1) peptide, human Noggin protein, transforming growth factor-β (TGF-β) type I receptor inhibitor SB431542, human fibroblast growth factor 7 (hFGF-7), hFGF-10, and glycogen synthase kinase 3 (GSK-3) inhibitor CHIR99021. Also disclosed is a method for preparing a three-dimensional cell culture of alveolar epithelium using the first medium and the second medium.