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公开(公告)号：US20110244498A1
公开(公告)日：2011-10-06
申请号：US13078494
申请日：2011-04-01
申请人： Neal J. Zondlo , Susan Carr Zondlo , Feng Gao
发明人： Neal J. Zondlo , Susan Carr Zondlo , Feng Gao
摘要： The present invention relates to a tyrosine kinase-inducible domain (pKID) and uses thereof. An isolated polypeptide comprising the pKID, and an isolated polynucleotide comprising a nucleic acid sequence encoding the pKID are provided. Also provided are methods for determining tyrosine kinase and/or phosphatase activity in a sample and for identifying an agent that inhibits a tyrosine kinase or phosphatase using a polypeptide comprising the pKID.
摘要翻译： 本发明涉及酪氨酸激酶诱导结构域（pKID）及其用途。 提供了包含pKID的分离的多肽和包含编码pKID的核酸序列的分离的多核苷酸。 还提供了用于在样品中测定酪氨酸激酶和/或磷酸酶活性并鉴定使用包含pKID的多肽来抑制酪氨酸激酶或磷酸酶的试剂的方法。
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公开(公告)号：US08551723B2
公开(公告)日：2013-10-08
申请号：US13078494
申请日：2011-04-01
申请人： Neal J. Zondlo , Susan Carr Zondlo , Feng Gao
发明人： Neal J. Zondlo , Susan Carr Zondlo , Feng Gao
摘要： The present invention relates to a tyrosine kinase-inducible domain (pKID) and uses thereof. An isolated polypeptide comprising the pKID, and an isolated polynucleotide comprising a nucleic acid sequence encoding the pKID are provided. Also provided are methods for determining tyrosine kinase and/or phosphatase activity in a sample and for identifying an agent that inhibits a tyrosine kinase or phosphatase using a polypeptide comprising the pKID.
摘要翻译： 本发明涉及酪氨酸激酶诱导结构域（pKID）及其用途。 提供了包含pKID的分离的多肽和包含编码pKID的核酸序列的分离的多核苷酸。 还提供了用于在样品中测定酪氨酸激酶和/或磷酸酶活性并鉴定使用包含pKID的多肽来抑制酪氨酸激酶或磷酸酶的试剂的方法。
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公开(公告)号：US20120010392A1
公开(公告)日：2012-01-12
申请号：US13168592
申请日：2011-06-24
IPC分类号： C12N15/11
CPC分类号： C12Q1/485 , C07H21/04 , C07K2319/90 , C12P21/02 , C12Q1/42 , G01N2500/10
摘要： Applicants have used protein design to develop novel functional protein architectures, termed protein kinase-inducible domains, whose structures are dependent on phosphorylation by specific protein kinases or are dependent on dephosphorylation by specific protein phosphatases. Applicants have designed kinase-inducible domains based on a modular architecture, which allows kinase-inducible domains to be responsive to any specific serine-threonine kinases. Kinase-inducible domains can consist of canonical amino acids, allowing their use as expressible tags of protein kinase activity.
摘要翻译： 申请人已经使用蛋白质设计开发新的功能蛋白质结构，称为蛋白激酶诱导结构域，其结构依赖于特异性蛋白激酶的磷酸化，或依赖于特异性蛋白磷酸酶的去磷酸化。 申请人已经设计了基于模块化结构的激酶诱导结构域，其允许激酶诱导型结构域对任何特定的丝氨酸 - 苏氨酸激酶反应。 激酶诱导型结构域可以由典型氨基酸组成，允许它们用作蛋白激酶活性的可表达标签。
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公开(公告)号：US08003759B2
公开(公告)日：2011-08-23
申请号：US12880638
申请日：2010-09-13
IPC分类号： C07K1/00
CPC分类号： C12Q1/485 , C07H21/04 , C07K2319/90 , C12P21/02 , C12Q1/42 , G01N2500/10
摘要： Applicants have used protein design to develop novel functional protein architectures, termed protein kinase-inducible domains, whose structures are dependent on phosphorylation by specific protein kinases or are dependent on dephosphorylation by specific protein phosphatases. Applicants have designed kinase-inducible domains based on a modular architecture, which allows kinase-inducible domains to be responsive to any specific serine-threonine kinases. Kinase-inducible domains can consist of canonical amino acids, allowing their use as expressible tags of protein kinase activity.
摘要翻译： 申请人已经使用蛋白质设计开发新的功能蛋白质结构，称为蛋白激酶诱导结构域，其结构依赖于特异性蛋白激酶的磷酸化，或依赖于特异性蛋白磷酸酶的去磷酸化。 申请人已经设计了基于模块化结构的激酶诱导结构域，其允许激酶诱导型结构域对任何特定的丝氨酸 - 苏氨酸激酶反应。 激酶诱导型结构域可以由典型氨基酸组成，允许它们用作蛋白激酶活性的可表达标签。
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公开(公告)号：US07816102B2
公开(公告)日：2010-10-19
申请号：US11731611
申请日：2007-03-30
CPC分类号： C12Q1/485 , C07H21/04 , C07K2319/90 , C12P21/02 , C12Q1/42 , G01N2500/10
摘要： Applicants have used protein design to develop novel functional protein architectures, termed protein kinase-inducible domains, whose structures are dependent on phosphorylation by specific protein kinases or are dependent on dephosphorylation by specific protein phosphatases. Applicants have designed kinase-inducible domains based on a modular architecture, which allows kinase-inducible domains to be responsive to any specific serine-threonine kinases. Kinase-inducible domains can consist of canonical amino acids, allowing their use as expressible tags of protein kinase activity.
摘要翻译： 申请人已经使用蛋白质设计开发新的功能蛋白质结构，称为蛋白激酶诱导结构域，其结构依赖于特异性蛋白激酶的磷酸化，或依赖于特异性蛋白磷酸酶的去磷酸化。 申请人已经设计了基于模块化结构的激酶诱导结构域，其允许激酶诱导型结构域对任何特定的丝氨酸 - 苏氨酸激酶反应。 激酶诱导型结构域可以由典型氨基酸组成，允许它们用作蛋白激酶活性的可表达标签。
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公开(公告)号：US08148506B2
公开(公告)日：2012-04-03
申请号：US13168592
申请日：2011-06-24
IPC分类号： C07H21/02
CPC分类号： C12Q1/485 , C07H21/04 , C07K2319/90 , C12P21/02 , C12Q1/42 , G01N2500/10
摘要： Applicants have used protein design to develop novel functional protein architectures, termed protein kinase-inducible domains, whose structures are dependent on phosphorylation by specific protein kinases or are dependent on dephosphorylation by specific protein phosphatases. Applicants have designed kinase-inducible domains based on a modular architecture, which allows kinase-inducible domains to be responsive to any specific serine-threonine kinases. Kinase-inducible domains can consist of canonical amino acids, allowing their use as expressible tags of protein kinase activity.
摘要翻译： 申请人已经使用蛋白质设计开发新的功能蛋白质结构，称为蛋白激酶诱导结构域，其结构依赖于特异性蛋白激酶的磷酸化，或依赖于特异性蛋白磷酸酶的去磷酸化。 申请人已经设计了基于模块化结构的激酶诱导结构域，其允许激酶诱导型结构域对任何特定的丝氨酸 - 苏氨酸激酶反应。 激酶诱导型结构域可以由典型氨基酸组成，允许它们用作蛋白激酶活性的可表达标签。
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公开(公告)号：US07297762B2
公开(公告)日：2007-11-20
申请号：US09840085
申请日：2001-04-24
申请人： Alanna Schepartz Shrader , Jason W. K. Chin , Reena Zutshi , Stacey E. Rutledge , Joanne D. Kehleck Martin , Neal J. Zondlo
发明人： Alanna Schepartz Shrader , Jason W. K. Chin , Reena Zutshi , Stacey E. Rutledge , Joanne D. Kehleck Martin , Neal J. Zondlo
IPC分类号： C07K14/465 , C07K7/00
CPC分类号： C40B40/02 , C07K14/00 , C07K14/465 , C07K2319/00 , C12N15/1037 , C12N15/62
摘要： The present invention provides a protein scaffold, such as an avian pancreatic polypeptide, that can be modified by substitution of two or more amino acid residues that are exposed on the alpha helix domain of the polypeptide when the polypeptide is in a tertiary form.
摘要翻译： 本发明提供蛋白质支架，例如禽胰腺多肽，当多肽处于三级形式时，可通过取代暴露于多肽的α螺旋结构域上的两个或更多个氨基酸残基来修饰。
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