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    Method to increase sensitivity of detection of low-occurrence mutations
    3.
    发明授权
    Method to increase sensitivity of detection of low-occurrence mutations 有权

    公开(公告)号：US10329605B2

    公开(公告)日：2019-06-25

    申请号：US15134302

    申请日：2016-04-20

    Applicant: NEOGENOMICS LABORATORIES, INC.

    Inventor： Maher Albitar

    IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6886

    Abstract: A method for detecting a low-occurrence mutation in isolated DNA adds a blocking probe to reagents during amplification of the isolated DNA. The blocking probe is an oligonucleotide complementary to wild-type DNA corresponding to the sample. The blocking probe spans a site of a suspected mutation within a region of interest in the isolated DNA. After amplification, fragments of the amplified DNA is sequenced using next generating sequencing and an output is generated to display the sequenced fragments. In some embodiments, the blocking probe is locked nucleic acid (LNA).

    HIGH-SENSITIVITY SEQUENCING TO DETECT BTK INHIBITOR RESISTANCE
    4.
    发明申请
    HIGH-SENSITIVITY SEQUENCING TO DETECT BTK INHIBITOR RESISTANCE 审中-公开
    检测BTK抑制剂电阻的高灵敏度

    公开(公告)号：US20170051357A1

    公开(公告)日：2017-02-23

    申请号：US15239542

    申请日：2016-08-17

    Applicant: Neogenomics Laboratories, Inc.

    Inventor： Maher Albitar

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156

    Abstract: A method for predicting resistance to BTK inhibitors in patients with chronic lymphocytic leukemia (CLL) enhances the sensitivity of Sanger sequencing and NGS by using wild-type blocking of genes that are relevant for detecting resistance to ibrutinib. Further enhancement of sensitivity can be achieved by using cell-free DNA.

    Abstract translation: 用于预测慢性淋巴细胞性白血病（CLL）患者中BTK抑制剂抗性的方法通过使用与检测对伊布他滨抗性相关的基因的野生型阻断来增强Sanger测序和NGS的敏感性。 通过使用无细胞DNA可以进一步提高灵敏度。

    METHOD FOR HIGH SENSITIVITY DETECTION OF MYD88 MUTATIONS
    5.
    发明申请
    METHOD FOR HIGH SENSITIVITY DETECTION OF MYD88 MUTATIONS 审中-公开
    MYD88突变检测敏感性检测方法

    公开(公告)号：US20170002427A1

    公开(公告)日：2017-01-05

    申请号：US15201302

    申请日：2016-07-01

    Applicant: Neogenomics Laboratories, Inc.

    Inventor： Maher Albitar

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/156

    Abstract: A method for increasing sensitivity for detecting minority mutations in MYD88 uses a locked nucleic acid oligo to block amplification of wild-type DNA in DNA isolated from patient FFPE tissue, bone marrow aspirate or peripheral blood samples during PCR while still allowing sequencing and visualization of the PCR product. Further improvement to the sensitivity may be achieved by using a uracil DNA-glycosylase treatment to remove sequence artifacts commonly found in formalin-fixed, paraffin-embedded tissue.

    Abstract translation: 用于检测MYD88中少数突变的灵敏度的方法使用锁定的核酸寡核苷酸来阻断在PCR期间分离自患者FFPE组织，骨髓抽吸物或外周血样品的DNA中的野生型DNA的扩增，同时仍允许测序和观察 PCR产物。 通过使用尿嘧啶DNA - 糖基化酶处理来消除通常在福尔马林固定的，石蜡包埋的组织中发现的序列假象，可以进一步改善敏感性。

    Method for high sensitivity detection of MYD88 mutations
    7.
    发明授权
    Method for high sensitivity detection of MYD88 mutations 有权

    公开(公告)号：US10227657B2

    公开(公告)日：2019-03-12

    申请号：US15201302

    申请日：2016-07-01

    Applicant: Neogenomics Laboratories, Inc.

    Inventor： Maher Albitar

    IPC: C12Q1/68 ,  C12Q1/6886

    Abstract: A method for increasing sensitivity for detecting minority mutations in MYD88 uses a locked nucleic acid oligo to block amplification of wild-type DNA in DNA isolated from patient FFPE tissue, bone marrow aspirate or peripheral blood samples during PCR while still allowing sequencing and visualization of the PCR product. Further improvement to the sensitivity may be achieved by using a uracil DNA-glycosylase treatment to remove sequence artifacts commonly found in formalin-fixed, paraffin-embedded tissue.

    METHOD TO INCREASE SENSITIVITY OF NEXT GENERATION SEQUENCING
    8.
    发明申请
    METHOD TO INCREASE SENSITIVITY OF NEXT GENERATION SEQUENCING 审中-公开
    提高下一代测序灵敏度的方法

    公开(公告)号：US20160340725A1

    公开(公告)日：2016-11-24

    申请号：US15134302

    申请日：2016-04-20

    Applicant: NEOGENOMICS LABORATORIES, INC.

    Inventor： Maher Albitar

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2537/163

    Abstract: A method for detecting a low-occurrence mutation in isolated DNA adds a blocking probe to reagents during amplification of the isolated DNA. The blocking probe is an oligonucleotide complementary to wild-type DNA corresponding to the sample. The blocking probe spans a site of a suspected mutation within a region of interest in the isolated DNA. After amplification, fragments of the amplified DNA is sequenced using next generating sequencing and an output is generated to display the sequenced fragments. In some embodiments, the blocking probe is locked nucleic acid (LNA).

    Abstract translation: 用于检测分离的DNA中的低发生突变的方法在扩增分离的DNA期间向试剂添加封闭探针。 阻断探针是与对应于样品的野生型DNA互补的寡核苷酸。 阻断探针跨越分离的DNA中感兴趣区域内的疑似突变的位点。 扩增后，使用下一个生成测序对扩增的DNA的片段进行测序，并产生输出以显示测序的片段。 在一些实施方案中，阻断探针是锁定核酸（LNA）。

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