公开(公告)号：US12104194B2

公开(公告)日：2024-10-01

申请号：US17398967

申请日：2021-08-10

Applicant: Integrated DNA Technologies, Inc.

Inventor： Vladimir Makarov ,  Laurie Kurihara

IPC: C12Q1/6855 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12P19/34 ,  C12N9/1241 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2521/131 ,  C12Q2525/121 ,  C12Q2525/173 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q1/6806 ,  C12Q2521/131 ,  C12Q2525/186 ,  C12Q2525/204 ,  C12Q2537/163 ,  C12Q1/6855 ,  C12Q2521/131 ,  C12Q2525/186 ,  C12Q2525/204

Abstract: The present invention is directed to methods and compositions for adding tails of specific lengths to a substrate polynucleotide. The invention also contemplates methods and compositions for immobilization of tailed substrates to a solid support. The disclosure contemplates that the attenuator molecule is any biomolecule that associates with a tail sequence added to a substrate polynucleotide and controls the addition of a tail sequence to the 3′ end of the substrate polynucleotide. The sequence that is added to the substrate polynucleotide is referred to herein as a tail sequence, or simply a tail, and the process of adding a nucleotide to a substrate polynucleotide is referred to herein as tailing.

公开(公告)号：US12054786B2

公开(公告)日：2024-08-06

申请号：US17730100

申请日：2022-04-26

Applicant: New Day Diagnostics, LLC

Inventor： Denise Kottwitz ,  Jorn Lewin ,  Anne Schlegel ,  Reimo Tetzner

IPC: C12Q1/6886 ,  C12Q1/6848 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C12Q1/6848 ,  C12Q1/6858 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q1/6858 ,  C12Q2523/125 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2525/204 ,  C12Q2537/163 ,  C12Q2537/164

Abstract: The present invention relates to the field of pharmacogenomics and in particular to detecting the presence or absence of hypermethylated DNA. The detection of CpG methylation in marker DNA is useful for the diagnosis of cancers and the invention provides improved methods for this purpose. These improved methods allow in particular for a more sensitive detection of methylated marker DNA with high backgrounds of unmethylated marker DNA.

公开(公告)号：US20230279468A1

公开(公告)日：2023-09-07

申请号：US18138284

申请日：2023-04-24

Applicant: Takara Bio USA, Inc.

Inventor： Kazuo Tori

IPC: C12Q1/6806

CPC classification number: C12Q1/6806 ,  C12Q2521/301 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2537/163

Abstract: Provided are methods of producing product nucleic acids involving the use of oligonucleotides that are modified by the application of a stimulus. Aspects of such methods may include producing product nucleic acids using de-activatable oligonucleotides that are deactivated by a de-activating stimulus, as well as methods that may include producing product nucleic acids using activatable oligonucleotides that are activated by an activating stimulus and de-activatable oligonucleotides that are deactivated by a de-activating stimulus. Also provided are kits, compositions and devices that include de-activatable oligonucleotides or activatable and de-activatable oligonucleotides, e.g., for use in performing the methods as described herein.

公开(公告)号：US11649486B2

公开(公告)日：2023-05-16

申请号：US16325247

申请日：2017-08-15

Applicant: Olink Bioscience AB

Inventor： Ulf Landegren ,  Johan Björkesten

IPC: C12Q1/6844 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6876 ,  C12Q1/6816 ,  C12Q1/6827

CPC classification number: C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2525/131 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2537/163 ,  C12Q1/6832 ,  C12Q2531/125 ,  C12Q2537/163

Abstract: Nucleic acid probes for detection of a target nucleic acid molecule by an RCA reaction in the presence of the target nucleic acid molecule, comprise a first circular template strand which is capable of acting as a template for RCA, and is protected from RCA in the absence of the target nucleic acid molecule by at least a second protector strand which comprises a region of complementarity to the first template strand and is hybridised thereto to form a double-stranded circular structure containing the first template strand inside the protector strand(s). At least one of the second and/or any further protector strands comprises a target binding site, such that upon binding of the probe to the target nucleic acid molecule the probe is able to undergo a strand displacement reaction which allows RCA of the first template strand. Methods of detecting target analytes use such probes.

公开(公告)号：US20180320225A1

公开(公告)日：2018-11-08

申请号：US15816979

申请日：2017-11-17

Applicant: Illumina, Inc.

Inventor： Kevin Gunderson ,  Frank Steemers

IPC: C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6837 ,  C12Q1/6816 ,  C12Q1/682

CPC classification number: C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6848 ,  C12Q2565/537 ,  C12Q2565/549 ,  C12Q2565/513 ,  C12Q2537/143 ,  C12Q2535/125 ,  C12Q2537/163 ,  C12Q2525/301 ,  C12Q2522/101

Abstract: This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.

公开(公告)号：US09970048B2

公开(公告)日：2018-05-15

申请号：US13774648

申请日：2013-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jian Gu ,  Kelli Bramlett ,  Christopher Burnett

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6848 ,  C12Q2521/501 ,  C12Q2525/113 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2537/163

Abstract: Disclosed are compositions and methods for the preparation of RNA libraries for sequencing, gene expression profiling, microarray and other uses and for simplification of the library preparation process. The disclosure provides blocking oligonucleotides which bind to byproduct nucleic acid molecules formed during the ligation of adapters to nucleic acid segments prior to sequencing and inhibit or block amplification of the byproduct nucleic acid molecules in subsequent amplification reactions. Methods for library preparation using blocking oligonucleotides are also provided.

公开(公告)号：US20180127814A1

公开(公告)日：2018-05-10

申请号：US15691337

申请日：2017-08-30

Applicant: Kabushiki Kaisha Toshiba

Inventor： Koji HASHIMOTO ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12N15/09 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q1/6851 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2537/1373 ,  C12Q2537/163 ,  C12Q2563/113 ,  C12Q2531/101

Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.

公开(公告)号：US09944979B2

公开(公告)日：2018-04-17

申请号：US13821968

申请日：2011-09-09

Applicant: Hiroshi Terasaki ,  Tsunetada Konno ,  Mitsunobu Shimadzu ,  Kenzo Fujimoto ,  Takashi Sakamoto

Inventor： Hiroshi Terasaki ,  Tsunetada Konno ,  Mitsunobu Shimadzu ,  Kenzo Fujimoto ,  Takashi Sakamoto

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6858 ,  C12Q2523/313 ,  C12Q2537/163

Abstract: Provided is a method for rapidly and easily detecting a mutated nucleic acid, which is contained in a small amount in a nucleic acid sample together with wild-type nucleic acids, with high specificity and high sensitivity. In the method of the present invention, amplification of a detection region comprising a target site by a nucleic acid amplification method is inhibited, by the steps of allowing a nucleic acid having a target site to coexist with a clamp probe comprising a photo-crosslinking nucleic acid and having a sequence complementary to the target site, and photo-crosslinking the nucleic acid having the target site with the clamp probe by photo-irradiation.

公开(公告)号：US20180002734A1

公开(公告)日：2018-01-04

申请号：US15634874

申请日：2017-06-27

Applicant: BioTex, Inc.

Inventor： George Jackson ,  Christopher Quan

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6804 ,  C12Q1/6844 ,  C12Q2525/205 ,  C12Q2537/161 ,  G01N33/5308 ,  C12Q2537/163 ,  C12Q2525/207

Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

公开(公告)号：US20170327868A1

公开(公告)日：2017-11-16

申请号：US15514852

申请日：2016-10-20

Applicant: Admera Health LLC

Inventor： Jun Huang ,  Danielle Quintanilha ,  Guanghui Hu ,  Yang Song

IPC: C12Q1/68 ,  C12Q1/00

CPC classification number: C12Q1/6806 ,  C12Q1/00 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2537/159 ,  C12Q2537/163

Abstract: The present invention relates to a PCR-based systems and methods for enrichment of minority alleles and mutations.