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公开(公告)号：US11732315B2
公开(公告)日：2023-08-22
申请号：US17936084
申请日：2022-09-28
发明人： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, Jr. , Clotilde Carlow , Esta Slayton , Thomas C. Evans, Jr.
摘要： Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity. The compositions and methods may use any single improvement or combination of improvements selected from thermolabile enzyme variants, poloxamers, various salts, indicators and one or more LAMP primer sets for detecting single and/or multiple targets, probes for detecting variants of the targets including SARS-CoV-2 variants and lateral flow devices.
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公开(公告)号：US11525166B2
公开(公告)日：2022-12-13
申请号：US17699950
申请日：2022-03-21
发明人： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, Jr. , Clotilde Carlow , Esta Slayton
摘要： Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
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公开(公告)号：US20220213564A1
公开(公告)日：2022-07-07
申请号：US17699950
申请日：2022-03-21
发明人： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, JR. , Clotilde Carlow , Esta Slayton
摘要： Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
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公开(公告)号：US11345970B2
公开(公告)日：2022-05-31
申请号：US17406959
申请日：2021-08-19
发明人： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, Jr. , Clotilde Carlow , Esta Slayton
摘要： Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
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公开(公告)号：US20210404024A1
公开(公告)日：2021-12-30
申请号：US17406959
申请日：2021-08-19
发明人： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel, D.Phil. , Ivan R. Correa, Jr. , Clotilde Carlow , Esta Slayton
摘要： Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
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公开(公告)号：US20210285064A1
公开(公告)日：2021-09-16
申请号：US17178395
申请日：2021-02-18
发明人： Nathan Tanner , Yinhua Zhang , Gregory Patton , Guoping Ren , Zhiru Li , Nicole Nichols
摘要： Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.
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公开(公告)号：US20190169234A1
公开(公告)日：2019-06-06
申请号：US16324424
申请日：2017-08-10
发明人： James Inglese , Patricia Dranchak , Ryan MacArthur , Hiroaki Suga , Hao Yu , Clotilde Carlow , Zhiru Li
摘要： Disclosed herein are isolated peptides inhibit activity of a cofactor-independent phosphoglycerate mutase. In some examples, the isolated peptide is 6-20 amino acids long and includes the amino acid sequence of any one of SEQ ID NOs: 1-22 or 54, an analog or derivative thereof, or a pharmaceutically acceptable salt or ester thereof. In some examples, the peptide is a cyclic peptide with an N-terminal ring of 6-15 amino acids (for example, 6-10 amino acids) and a C-terminal linear portion of 1-9 amino acids (for example, 3-8 amino acids. Also disclosed h are methods of treating or inhibiting an infection in a subject, including administering to the subject an effective amount of a composition including one of more of the disclosed peptides, or analogs or derivative thereof, or pharmaceutically acceptable salts or esters thereof.
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公开(公告)号：US11492673B2
公开(公告)日：2022-11-08
申请号：US17178395
申请日：2021-02-18
发明人： Nathan Tanner , Yinhua Zhang , Gregory Patton , Guoping Ren , Zhiru Li , Nicole Nichols
IPC分类号： C12Q1/70 , G01N21/78 , C12Q1/6844
摘要： Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.
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公开(公告)号：US11155887B2
公开(公告)日：2021-10-26
申请号：US17221451
申请日：2021-04-02
发明人： Nathan Tanner , Yinhua Zhang , Gregory Patton , Guoping Ren , Zhiru Li , Nicole Nichols
摘要： Kits and methods are provided for performing multiplex Loop-Mediated Isothermal Amplification (LAMP) reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.
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公开(公告)号：US20210285065A1
公开(公告)日：2021-09-16
申请号：US17221451
申请日：2021-04-02
发明人： Nathan Tanner , Yinhua Zhang , Gregory Patton , Guoping Ren , Zhiru Li , Nicole Nichols
摘要： Kits and methods are provided for performing multiplex Loop-Mediated Isothermal Amplification (LAMP) reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.
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