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公开(公告)号：US11827885B1
公开(公告)日：2023-11-28
申请号：US17207507
申请日：2021-03-19
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Jennifer Ong , Esta Slayton , Lisa Maduzia , Salvatore V. Russello
IPC: C12N15/11 , C12N15/113 , C12N15/10 , C12N9/12 , C12Q1/6848 , C12N9/58
CPC classification number: C12N15/1137 , C12N9/1247 , C12N9/1276 , C12N15/1003 , C12Q1/6848 , C12N9/58 , C12N2310/16 , C12Y207/07006 , C12Y207/07049 , C12Q1/6848 , C12Q2525/205 , C12Q2527/127
Abstract: Compositions, methods and kits are provided that include an inhibitory oligonucleotide RNase inhibitor capable of inhibiting one or more types of RNase that coexist with biological samples or are introduced in the laboratory, thereby protecting RNA in the sample from degradation. More than one type of oligonucleotide RNase inhibitor may be combined in a mixture to inhibit a plurality of different RNases. Single oligonucleotides were identified to have inhibitory activity for a plurality of different RNases. The RNase oligonucleotide inhibitor may be immobilized on beads or other surface. It may be stored in a lyophilized form or in solution.
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公开(公告)号：US20220213564A1
公开(公告)日：2022-07-07
申请号：US17699950
申请日：2022-03-21
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, JR. , Clotilde Carlow , Esta Slayton
Abstract: Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
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公开(公告)号：US11345970B2
公开(公告)日：2022-05-31
申请号：US17406959
申请日：2021-08-19
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, Jr. , Clotilde Carlow , Esta Slayton
Abstract: Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
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公开(公告)号：US20180171399A1
公开(公告)日：2018-06-21
申请号：US15888437
申请日：2018-02-05
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Thomas C. Evans, JR.
IPC: C12Q1/6853 , C12Q1/6848
CPC classification number: C12Q1/6853 , C12Q1/6848 , C12Q2521/513 , C12Q2527/101
Abstract: Provided herein is a method for reducing amplification of non-template molecules in a nucleic acid sample. In certain embodiments, the method involves adding a helicase to a reaction mixture for non-helicase-dependent amplification of target nucleic acid.
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公开(公告)号：US09546358B2
公开(公告)日：2017-01-17
申请号：US13799463
申请日：2013-03-13
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Thomas C. Evans
CPC classification number: C12N9/1241 , C12Q1/6848 , C12Q2527/125
Abstract: Compositions are provided that include a plurality of small molecules selected from the group consisting of an amide, urea or acetone having a molecular weight less than 300 g/mol; and dNTPs and a polymerase in a buffer suitable for use as an amplification buffer. Methods of use of the compositions are also described for reducing non-template DNA amplification.
Abstract translation: 提供了包含选自分子量小于300g / mol的酰胺，脲或丙酮的多个小分子的组合物; 和dNTP和在适合用作扩增缓冲液的缓冲液中的聚合酶。 还描述了用于减少非模板DNA扩增的组合物的使用方法。
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Patent Agency Ranking
公开(公告)号：US11732315B2
公开(公告)日：2023-08-22
申请号：US17936084
申请日：2022-09-28
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, Jr. , Clotilde Carlow , Esta Slayton , Thomas C. Evans, Jr.
Abstract: Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity. The compositions and methods may use any single improvement or combination of improvements selected from thermolabile enzyme variants, poloxamers, various salts, indicators and one or more LAMP primer sets for detecting single and/or multiple targets, probes for detecting variants of the targets including SARS-CoV-2 variants and lateral flow devices.
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公开(公告)号：US11525166B2
公开(公告)日：2022-12-13
申请号：US17699950
申请日：2022-03-21
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Yinhua Zhang , Eric Hunt , Gregory Patton , Guoping Ren , Zhiru Li , Andrew Barry , Nicole Nichols , Catherine B. Poole , Harriet M. Strimpel , Ivan R. Correa, Jr. , Clotilde Carlow , Esta Slayton
Abstract: Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
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公开(公告)号：US11162133B2
公开(公告)日：2021-11-02
申请号：US16306319
申请日：2017-05-12
Applicant: New England Biolabs, Inc.
Inventor： Yinhua Zhang , Nathan Tanner , Thomas C. Evans, Jr.
IPC: C12Q1/68 , C12Q1/6844 , C12Q1/6816 , C12Q1/6876 , G01N33/52
Abstract: Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and Tris buffer in the range of 1.5 mM Tris to 5 mM Tris or equivalent.
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公开(公告)号：US09920358B2
公开(公告)日：2018-03-20
申请号：US14385674
申请日：2014-06-27
Applicant: New England Biolabs, Inc.
Inventor： Nathan Tanner , Thomas C. Evans, Jr.
CPC classification number: C12Q1/6853 , C12Q1/6848 , C12Q2521/513 , C12Q2527/101
Abstract: Provided herein is a method for reducing amplification of non-template molecules in a nucleic acid sample. In certain embodiments, the method involves adding a helicase to a reaction mixture for non-helicase-dependent amplification of target nucleic acid.
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公开(公告)号：US20160237483A1
公开(公告)日：2016-08-18
申请号：US14385674
申请日：2014-06-27
Applicant: NEW ENGLAND BIOLABS, INC.
Inventor： Nathan Tanner , Thomas C. Evans, JR.
IPC: C12Q1/68
CPC classification number: C12Q1/6853 , C12Q1/6848 , C12Q2521/513 , C12Q2527/101
Abstract: Provided herein is a method for reducing amplification of non-template molecules in a nucleic acid sample. In certain embodiments, the method involves adding a helicase to a reaction mixture for non-helicase-dependent amplification of target nucleic acid.
Abstract translation: 本文提供的是减少核酸样品中非模板分子的扩增的方法。 在某些实施方案中，该方法包括向反应混合物中加入解旋酶以进行靶向核酸的非解旋酶依赖性扩增。
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