公开(公告)号：US20230407419A1

公开(公告)日：2023-12-21

申请号：US18452104

申请日：2023-08-18

申请人： New England Biolabs, Inc.

发明人： Nathan Tanner ,  Yinhua Zhang ,  Eric Hunt ,  Gregory Patton ,  Guoping Ren ,  Zhiru Li ,  Andrew Barry ,  Nicole Nichols ,  Catherine B. Poole ,  Harriet M. Strimpel ,  Ivan R. Correa, Jr. ,  Clotilde Carlow ,  Esta Slayton ,  Thomas C. Evans, Jr.

IPC分类号： C12Q1/70 ,  G01N21/78

CPC分类号： C12Q1/701 ,  G01N21/78

摘要： Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity. The compositions and methods may use any single improvement or combination of improvements selected from thermolabile enzyme variants, poloxamers, various salts, indicators and one or more LAMP primer sets for detecting single and/or multiple targets, probes for detecting variants of the targets including SARS-CoV-2 variants and lateral flow devices.

公开(公告)号：US20230048863A1

公开(公告)日：2023-02-16

申请号：US17936084

申请日：2022-09-28

申请人： New England Biolabs, Inc.

发明人： Nathan Tanner ,  Yinhua Zhang ,  Eric Hunt ,  Gregory Patton ,  Guoping Ren ,  Zhiru Li ,  Andrew Barry ,  Nicole Nichols ,  Catherine B. Poole ,  Harriet M. Strimpel ,  Ivan R. Correa, JR. ,  Clotilde Carlow ,  Esta Slayton ,  Thomas C. Evans, JR.

IPC分类号： C12Q1/70 ,  G01N21/78

摘要： Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity. The compositions and methods may use any single improvement or combination of improvements selected from thermolabile enzyme variants, poloxamers, various salts, indicators and one or more LAMP primer sets for detecting single and/or multiple targets, probes for detecting variants of the targets including SARS-CoV-2 variants and lateral flow devices.

公开(公告)号：US11008629B1

公开(公告)日：2021-05-18

申请号：US17122979

申请日：2020-12-15

申请人： New England Biolabs, Inc.

发明人： Nathan Tanner ,  Yinhua Zhang ,  Gregory Patton ,  Guoping Ren ,  Zhiru Li ,  Nicole Nichols

IPC分类号： G01N21/78 ,  C12Q1/70

摘要： Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.

公开(公告)号：US20180223276A1

公开(公告)日：2018-08-09

申请号：US15947971

申请日：2018-04-09

申请人： New England Biolabs, Inc.

发明人： Shengxi Guan ,  Thomas C. Evans, JR. ,  Nicole Nichols ,  Yanxia Bei

IPC分类号： C12N15/10 ,  C12Q1/6848 ,  C12N9/12

CPC分类号： C12N15/1096 ,  C12N9/1276 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Y207/07049 ,  C12Q2521/107 ,  C12Q2521/301 ,  C12Q2525/131 ,  C12Q2525/151 ,  C12Q2525/155

摘要： Compositions and methods for performing a template-switching reaction are provided that may include reducing or eliminating concatemerization of the template-switching oligonucleotide (TSO). In some embodiments, the composition may comprise: a reverse transcriptase; a TSO that includes a recognition sequence for a site-specific double strand nucleic acid cleaving enzyme, wherein the TSO has at its 3′ end at least one nucleotide capable of hybridizing to at least one or more non-templated nucleotides added to a templated cDNA strand by the reverse transcriptase; and a site-specific double strand nucleic acid cleaving enzyme that cleaves the TSO at the recognition sequence.

公开(公告)号：US20240327807A1

公开(公告)日：2024-10-03

申请号：US18742500

申请日：2024-06-13

申请人： New England Biolabs, Inc.

发明人： Guoping Ren ,  Yan Xu ,  Dong Ma ,  Nicole Nichols

IPC分类号： C12N9/12 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686

CPC分类号： C12N9/1276 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2521/107 ,  C12Q2521/119 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Y207/07049

摘要： Provided herein is a reverse transcriptase mixture comprising a reverse transcriptase and a colored dye at a concentration in the range of 0.003%-1% (v/w). The colored dye may be visually observed during transfer of the mix from one vessel to another and addition of the mix to another mix can be confirmed by eye by observing the colored dye.

公开(公告)号：US20220213564A1

公开(公告)日：2022-07-07

申请号：US17699950

申请日：2022-03-21

申请人： New England Biolabs, Inc.

发明人： Nathan Tanner ,  Yinhua Zhang ,  Eric Hunt ,  Gregory Patton ,  Guoping Ren ,  Zhiru Li ,  Andrew Barry ,  Nicole Nichols ,  Catherine B. Poole ,  Harriet M. Strimpel ,  Ivan R. Correa, JR. ,  Clotilde Carlow ,  Esta Slayton

IPC分类号： C12Q1/70 ,  G01N21/78

摘要： Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.

公开(公告)号：US11345970B2

公开(公告)日：2022-05-31

申请号：US17406959

申请日：2021-08-19

申请人： New England Biolabs, Inc.

发明人： Nathan Tanner ,  Yinhua Zhang ,  Eric Hunt ,  Gregory Patton ,  Guoping Ren ,  Zhiru Li ,  Andrew Barry ,  Nicole Nichols ,  Catherine B. Poole ,  Harriet M. Strimpel ,  Ivan R. Correa, Jr. ,  Clotilde Carlow ,  Esta Slayton

IPC分类号： G01N21/78 ,  C12Q1/70

摘要： Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.

公开(公告)号：US20180037884A1

公开(公告)日：2018-02-08

申请号：US15240166

申请日：2016-08-18

申请人： New England Biolabs, Inc.

发明人： Shengxi Guan ,  Thomas C. Evans, JR. ,  Nicole Nichols ,  Yanxia Bei

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12N9/12

CPC分类号： C12N15/1096 ,  C12N9/1276 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Y207/07049 ,  C12Q2521/107 ,  C12Q2521/301 ,  C12Q2525/131 ,  C12Q2525/151 ,  C12Q2525/155

摘要： Compositions and methods for performing a template-switching reaction are provided that may include reducing or eliminating concatemerization of the template-switching oligonucleotide (TSO). In some embodiments, the composition may comprise: a reverse transcriptase; a TSO that includes a recognition sequence for a site-specific double strand nucleic acid cleaving enzyme, wherein the TSO has at its 3′ end at least one nucleotide capable of hybridizing to at least one or more non-templated nucleotides added to a templated cDNA strand by the reverse transcriptase; and a site-specific double strand nucleic acid cleaving enzyme that cleaves the TSO at the recognition sequence.

公开(公告)号：US20210404024A1

公开(公告)日：2021-12-30

申请号：US17406959

申请日：2021-08-19

申请人： New England Biolabs, Inc.

发明人： Nathan Tanner ,  Yinhua Zhang ,  Eric Hunt ,  Gregory Patton ,  Guoping Ren ,  Zhiru Li ,  Andrew Barry ,  Nicole Nichols ,  Catherine B. Poole ,  Harriet M. Strimpel, D.Phil. ,  Ivan R. Correa, Jr. ,  Clotilde Carlow ,  Esta Slayton

IPC分类号： C12Q1/70 ,  G01N21/78

摘要： Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.

公开(公告)号：US20210285064A1

公开(公告)日：2021-09-16

申请号：US17178395

申请日：2021-02-18

申请人： New England Biolabs, Inc.

发明人： Nathan Tanner ,  Yinhua Zhang ,  Gregory Patton ,  Guoping Ren ,  Zhiru Li ,  Nicole Nichols

IPC分类号： C12Q1/70 ,  G01N21/78

摘要： Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.