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公开(公告)号:US09200308B2
公开(公告)日:2015-12-01
申请号:US14343684
申请日:2012-09-07
CPC分类号: C12Q1/006 , C12N9/0006 , C12Y101/03004
摘要: The present invention relates to mutants of the Penicillium amagasakiense glucose oxidase (GOx) enzyme which are of use for assaying glucose and to the development in particular of glucose electrodes and of biocells which use glucose as fuel.
摘要翻译: 本发明涉及用于测定葡萄糖和特别是葡萄糖电极的发展以及使用葡萄糖作为燃料的生物细胞的青霉青霉葡萄糖氧化酶(GOx)酶的突变体。
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2.
公开(公告)号:US20140305809A1
公开(公告)日:2014-10-16
申请号:US14343684
申请日:2012-09-07
IPC分类号: C12Q1/00
CPC分类号: C12Q1/006 , C12N9/0006 , C12Y101/03004
摘要: The present invention relates to mutants of the Penicillium amagasakiense glucose oxidase (GOx) enzyme which are of use for assaying glucose and to the development in particular of glucose electrodes and of biocells which use glucose as fuel.
摘要翻译: 本发明涉及用于测定葡萄糖和特别是葡萄糖电极的发展以及使用葡萄糖作为燃料的生物细胞的青霉青霉葡萄糖氧化酶(GOx)酶的突变体。
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公开(公告)号:US20050130262A1
公开(公告)日:2005-06-16
申请号:US10484380
申请日:2002-07-12
申请人: Bertrand Lambolez , Nathalie Gibelin , Gaelle Bourout , Ludovic Tricoire , Olivier Courjean , Keisuke Tsuzuki , Jean Rossier
发明人: Bertrand Lambolez , Nathalie Gibelin , Gaelle Bourout , Ludovic Tricoire , Olivier Courjean , Keisuke Tsuzuki , Jean Rossier
IPC分类号: C07K14/435 , C12N15/12 , C07H21/04 , C12N5/02
CPC分类号: C07K14/43595
摘要: Mutated photoproteins derived from isolated jellyfish photoproteins, characterised in that they exhibit a thermostability higher than that of the photoproteins from which they are derived, and are called thermostable mutated photoproteins, and/or a luminescence duration longer than that of the photoproteins from which they are derived, and are called persistent mutated photoproteins. The invention also concerns the use of said mutated photoproteins in methods for detecting in vitro molecules in a biological sample, methods for detecting compounds with enzymatic activity in a biological sample, or methods for detecting intracellular calcium variations.
摘要翻译: 衍生自分离的水母光蛋白的突变的光蛋白,其特征在于它们的热稳定性高于其衍生的光蛋白的热稳定性,并且被称为耐热突变的光蛋白,和/或发光持续时间长于它们的光蛋白的发光持续时间 衍生,被称为持续突变的光蛋白。 本发明还涉及所述突变的光蛋白在用于检测生物样品中的体外分子的方法中的用途,用于检测生物样品中具有酶活性的化合物的方法或用于检测细胞内钙变化的方法。
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