公开(公告)号：US06841360B2

公开(公告)日：2005-01-11

申请号：US10608504

申请日：2003-06-30

申请人： Nicole Kennerknecht ,  Hermann Sahm ,  Lothar Eggeling ,  Walter Pfefferie

发明人： Nicole Kennerknecht ,  Hermann Sahm ,  Lothar Eggeling ,  Walter Pfefferie

IPC分类号： C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12N15/54 ,  C12P13/06 ,  C12P13/08 ,  C12R1/15 ,  C12P21/06

CPC分类号： C07K14/34 ,  C12P13/06 ,  C12P13/08

摘要： This invention relates to isolated polynucleotides containing at least one of the polynucleotide sequences selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing at least one amino acid sequence of SEQ ID no. 3 or 5, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 3 or 5, c) polynucleotide which is complementary to the polynucleotides of a), b) or c), and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequences of a), b) or c). wherein the polypeptides exhibit the biological activity of the enzymes for which the brnE or bernF (sic)gene codes and a process for the fermentative production of branched-chain L-amino acids with amplification of the stated genes.

摘要翻译： 本发明涉及分离的多核苷酸，其含有至少一种多核苷酸序列，所述多核苷酸序列选自a组多核苷酸，所述多核苷酸序列与编码含有至少一个氨基酸序列SEQ ID NO：1的多核苷酸至少70％相同。 3或5，b）编码多肽的多核苷酸，其含有与SEQ ID No.3的氨基酸序列至少70％相同的氨基酸序列。 3或5，c）与a），b）或c）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）的多核苷酸序列的至少15个连续碱基的多核苷酸。 brnE或bernF（sic）基因编码的酶的生物活性以及用所述基因扩增产生支链L-氨基酸的方法。