公开(公告)号：US08119374B2

公开(公告)日：2012-02-21

申请号：US12136190

申请日：2008-06-10

Applicant: Petra Peters-Wendisch ,  Roman Netzer ,  Lothar Eggeling ,  Hermann Sahm

Inventor： Petra Peters-Wendisch ,  Roman Netzer ,  Lothar Eggeling ,  Hermann Sahm

IPC: C12P13/06 ,  C12P21/06 ,  C12N9/88

CPC classification number: C12N9/88 ,  C12P13/06

Abstract: This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.

Abstract translation: 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。

公开(公告)号：US20100261233A1

公开(公告)日：2010-10-14

申请号：US12249092

申请日：2008-10-10

Applicant: Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

Inventor： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC: C12P13/08

CPC classification number: C12N15/52 ,  C07K14/34 ,  C12P13/08

Abstract: L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract translation: 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过表达，以及制备L-赖氨酸的方法。

公开(公告)号：US20080153139A1

公开(公告)日：2008-06-26

申请号：US11663984

申请日：2006-04-06

Applicant: Jan Marienhagen ,  Lothar Eggeling ,  Hermann Sahm

Inventor： Jan Marienhagen ,  Lothar Eggeling ,  Hermann Sahm

IPC: C12P13/08 ,  C12N1/21

CPC classification number: C12P13/08 ,  C12N9/1096

Abstract: The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacteriuim glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.

Abstract translation: 本发明涉及L-缬氨酸和合适微生物的生产方法。 本发明的方法的特征在于优选增强棒状细菌，特别是谷氨酸棒杆菌（Corynebacteriuim glutamicum）的转氨酶C活性。 如此修饰的生物体在L-缬氨酸中的产率高于非改性生物体的35.8％。

公开(公告)号：US20070054380A1

公开(公告)日：2007-03-08

申请号：US11425091

申请日：2006-06-19

Applicant: Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

Inventor： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC: C12P13/08 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

CPC classification number: C12N15/52 ,  C07K14/34 ,  C12P13/08

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract translation: 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US07094584B2

公开(公告)日：2006-08-22

申请号：US10804120

申请日：2004-03-19

Applicant: Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

Inventor： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC: C12P13/08

CPC classification number: C12N15/52 ,  C07K14/34 ,  C12P13/08

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract translation: 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US06913909B2

公开(公告)日：2005-07-05

申请号：US09963521

申请日：2001-09-27

Applicant: Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach

Inventor： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach

IPC: C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/01 ,  C12N15/31 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06 ,  C12Q1/48

CPC classification number: C07K14/34 ,  C12P13/08

Abstract: The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out: a) Fermentation of microorganisms in which at least the thrE gene is amplified (overexpressed), optionally in combination with further genes, b) Enrichment of the L-threonine in the medium or in the cells of the microorganisms, and c) Isolation of the L-threonine.

Abstract translation: 本发明涉及优选来源于棒状杆菌的重组DNA，并可在包含编码thrE基因的至少一个核苷酸序列的棒状细菌中复制，以及L-苏氨酸的制备方法，其特征在于以下步骤为 进行：a）至少将thrE基因扩增（过表达）的微生物发酵，任选与其它基因组合，b）在培养基中或微生物细胞中富集L-苏氨酸，和c） L-苏氨酸的分离。

公开(公告)号：US06562607B2

公开(公告)日：2003-05-13

申请号：US09848726

申请日：2001-05-04

Applicant: Madhavan Nampoothiri K. ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm

Inventor： Madhavan Nampoothiri K. ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm

IPC: C12N900

CPC classification number: C12N9/1288 ,  C12P13/14

Abstract: This invention relates to a genetically modified coryneform bacterium, the cls gene of which is amplified, and to an isolated polynucleotide, which codes for cardiolipin synthase from coryneform bacteria and to a process for the fermentative production of L-amino acids with amplification of the cls gene in the bacteria and to the use of the polynucleotide as a primer or hybridization probe.

Abstract translation: 本发明涉及基因修饰的棒状细菌，其cls基因被扩增，并且分离的多核苷酸编码来自棒状细菌的心磷脂合成酶，以及用于扩增cls的L-氨基酸的发酵生产方法 基因和使用多核苷酸作为引物或杂交探针。

公开(公告)号：US6107063A

公开(公告)日：2000-08-22

申请号：US669378

申请日：1997-03-20

Applicant: Bettina Moeckel ,  Lothar Eggeling ,  Hermann Sahm

Inventor： Bettina Moeckel ,  Lothar Eggeling ,  Hermann Sahm

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/88 ,  C12N15/00 ,  C12N15/60 ,  C12P13/06 ,  C12R1/15 ,  C12N15/31 ,  C12N15/63

CPC classification number: C12N9/88 ,  C12P13/06

Abstract: The invention relates to processes for the microbial production of L-isoleucine. To this end, in a gene in vitro of a threonine dehydratse, one or more bases in the gene region coding the enzyme's allosteric domains are exchanged in such a way that at least one amino acid in the amino acid sequence of the allosteric domains of the enzyme is replaced by another so that the enzyme is no longer inhibited by L-isoleucine feedback. Furthermore, concrete amino acid exchanges in the amino acid sequence of the enzyme are effected in a gene in vitro of a threonin dehydratase of Corynebacterium glutamicum by base exchange both outside and inside and outside the gene region coding the allosteric domains of the enzyme si that, after the transformation of such mutated threonine dehydratase genes into a threonine or L-isoleucine-producing host cell, the latter repeatedly forms L-isoleucine.

Abstract translation: PCT No.PCT / DE95 / 00017 Sec。 371日期1997年3月20日 102（e）1997年3月20日PCT PCT 1995年1月9日PCT公布。 WO95 / 19442 PCT公开号 日期1995年7月20日本发明涉及L-异亮氨酸的微生物生产方法。 为此，在苏氨酸脱水体外的基因的基因中，编码酶变构域的基因区域中的一个或多个碱基以这样的方式交换，使得至少一个氨基酸序列的变构域 酶被另一个替代，使得酶不再被L-异亮氨酸反馈抑制。 此外，酶的氨基酸序列中的具体氨基酸交换通过在编码酶si的变构结构域的基因区域的外部和外部的碱基交换体外在谷氨酸棒杆菌的苏氨酸脱水酶的基因中进行， 在将这种突变的苏氨​​酸脱水酶基因转化成产生苏氨酸或L-异亮氨酸的宿主细胞后，后者重复形成L-异亮氨酸。

公开(公告)号：US09234218B2

公开(公告)日：2016-01-12

申请号：US12602593

申请日：2008-05-30

Applicant: Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

Inventor： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

IPC: C12P7/42 ,  C12P7/52 ,  C12N9/02 ,  C12P7/40 ,  C12P7/62 ,  C08F120/06 ,  C08F120/10 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88

CPC classification number: C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

Abstract: The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.

Abstract translation: 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

公开(公告)号：US20130310458A1

公开(公告)日：2013-11-21

申请号：US13695769

申请日：2011-05-03

Applicant: Lothar Eggeling ,  Michael Bott ,  Stephan Binder ,  Julia Frunzke ,  Nurije Mustafi

Inventor： Lothar Eggeling ,  Michael Bott ,  Stephan Binder ,  Julia Frunzke ,  Nurije Mustafi

IPC: C12P13/08 ,  C12N15/67

CPC classification number: C12Q1/6897 ,  C12N15/115 ,  C12N15/63 ,  C12N15/67 ,  C12N15/70 ,  C12N15/77 ,  C12N2310/16 ,  C12N2310/3519 ,  C12P13/08 ,  C12Q1/689 ,  C12Q2600/156

Abstract: The present invention relates to a cell which is genetically modified with respect to its wild type and which comprises a gene sequence coding for an autofluorescent protein, wherein the expression of the autofluorescent protein depends on the intracellular concentration of a particular metabolite.The present invention also relates to a method for the identification of a cell having an increased intracellular concentration of a particular metabolite, a method for the production of a cell which is genetically modified with respect to its wild type with optimized production of a particular metabolite, a cell obtained by this method, a method for the production of metabolites and a method for the preparation of a mixture.

Abstract translation: 本发明涉及对其野生型进行遗传修饰的细胞，其包含编码自体荧光蛋白的基因序列，其中所述自体荧光蛋白的表达取决于特定代谢物的细胞内浓度。 本发明还涉及一种用于鉴定具有增加的特定代谢物的细胞内浓度的细胞的方法，用于生产相对于其野生型经遗传修饰的细胞的方法，优化了特定代谢物的产生， 通过该方法获得的细胞，生产代谢物的方法和制备混合物的方法。