专利检索 ap:("Noboru Tomioka" OR "Fumio Omae" OR "Eriko Mine" OR "Tomoko Ishii") AND inv:"Eriko Mine" 第 1 页

1.

发明授权
Animal cell transformant, method for obtaining the transformant and method for producing desired foreign gene product by using the transformant 失效
标题翻译：动物细胞转化体，获得转化体的方法和使用变体生产所需外源基因产物的方法

公开(公告)号：US5219738A

公开(公告)日：1993-06-15

申请号：US45512

申请日：1987-05-04

申请人： Noboru Tomioka , Fumio Omae , Eriko Mine , Tomoko Ishii

发明人： Noboru Tomioka , Fumio Omae , Eriko Mine , Tomoko Ishii

IPC分类号： C12N9/50 , A61K38/00 , C12N5/00 , C12N5/10 , C12N9/64 , C12N9/72 , C12N15/00 , C12N15/09 , C12N15/85 , C12P21/00 , C12R1/91

CPC分类号： C12N9/6459 , C12N15/85 , C12Y304/21069 , A61K38/00

摘要： By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration of 50 .mu.M or lower, both genes can be amplified effectively within an MTX resistant strain and a stable amplified transformant can also be obtained. The screening may be repeated using an increasing concentration of MTX. It is also possible to obtain stably the desired foreign gene product in a high yield by using the thus-obtained amplified transformant.