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1.发明授权Alkaline lipase, method for producing the same, microorganism producing the same and detergent composition containing alkaline lipase 失效碱性脂肪酶，其制造方法，生产该微生物的微生物和含有碱性脂肪酶的洗涤剂组合物
公开(公告)号：US5454971A
公开(公告)日：1995-10-03
申请号：US67075
申请日：1993-05-26
申请人： Nobuaki Sakai , Masahiro Suzuki , Tatsuya Mizukoshi , Yukie Goto , Keijitsu Tanaka , Michihiro Takama , Norio Moriya , Kazunori Sakimoto
发明人： Nobuaki Sakai , Masahiro Suzuki , Tatsuya Mizukoshi , Yukie Goto , Keijitsu Tanaka , Michihiro Takama , Norio Moriya , Kazunori Sakimoto
CPC分类号： C12R1/38 , C11D3/38627 , C12N9/20 , Y10S435/874
摘要： A microorganism belonging to the genus Pseudomonas; an alkaline lipase produced by the microorganism or its mutants; a method of producing the alkaline lipase; and detergents containing the alkaline lipase as an aid, the alkaline lipase having (1) an operative pH of from 4 to 11.5, and an optimum pH of from 7.0 to 9.5, as measured using triolein emulsion as a substrate; (2) an operative temperature of from 10.degree. to 80.degree. C., and an optimum temperature of from 55.degree. to 65.degree. C., as measured using triolein emulsion as a substrate; (3) a molecular weight of 28,000 .+-.2,000 as measured by electrophoresis using SDS polyacrylamide; (4) isoelectric point of 4.5 .+-.1.5 as measured by isoelectric focusing polyacrylamide gel electrophoresis; and (5) an inhibition of lipase activity by a detergent component of not higher than 50% as measured using sodium linear-alkylbenzenesulfonate as said detergent component.The alkaline lipase has an improved stability against detergent components such as a surfactant and a protease, and can be blended with a detergent together with a protease. The alkaline lipase is less susceptible to inhibition of lipase activity by a detergent component and can increase washing power of detergents.
摘要翻译： 属于假单胞菌属的微生物; 由微生物或其突变体产生的碱性脂肪酶; 一种生产碱性脂肪酶的方法; 和含有碱性脂肪酶的洗涤剂作为辅助剂，所述碱性脂肪酶具有（1）操作pH为4至11.5，最佳pH为7.0至9.5，使用三油酸酯乳液作为底物; （2）使用三油精乳液作为底物测定的操作温度为10℃至80℃，最适温度为55℃至65℃; （3）通过使用SDS聚丙烯酰胺的电泳测量的分子量为28,000 +/- 2,000; （4）通过等电聚焦聚丙烯酰胺凝胶电泳测量的等电点为4.5 +/- 1.5; 和（5）使用直链烷基苯磺酸钠作为所述洗涤剂组分测定的洗涤剂组分对脂肪酶活性的抑制不高于50％。 碱性脂肪酶对洗涤剂组分如表面活性剂和蛋白酶具有改善的稳定性，并且可与蛋白酶一起与洗涤剂共混。 碱性脂肪酶不太容易被洗涤剂组分抑制脂肪酶活性，并且可以提高洗涤剂的洗涤能力。
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