利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.02 秒)

    Generation of novel metabolite-responsive transcription regulator biosensors
    3.
    发明授权
    Generation of novel metabolite-responsive transcription regulator biosensors 有权

    公开(公告)号：US10604552B2

    公开(公告)日：2020-03-31

    申请号：US16342777

    申请日：2017-10-17

    申请人： NORTHWESTERN UNIVERSITY

    发明人： Joshua N. Leonard ,  Andrew K. D. Younger ,  Keith E. J. Tyo

    IPC分类号： C07K14/47 ,  C12N15/10 ,  C12Q1/6897 ,  G01N33/566

    摘要： Disclosed are systems, components, and methods for sensing a ligand in a cell or a reaction mixture. The disclosed systems, components, and methods may include and/or utilize a fusion protein comprising a ligand-binding protein and a DNA-binding protein. The fusion protein binds the ligand of the ligand-binding protein and modulates expression of a reporter gene operably linked to a promoter that is engineered to include specific binding sites for the DNA-binding protein. The difference in expression of the reporter gene in the presence of the ligand versus expression of the reporter gene in the absence of the ligand can be correlated to the concentration of the ligand in a reaction mixture.

    Targeted and modular exosome loading system
    4.
    发明授权
    Targeted and modular exosome loading system 有权

    公开(公告)号：US10538570B2

    公开(公告)日：2020-01-21

    申请号：US14502494

    申请日：2014-09-30

    申请人： Northwestern University

    发明人： Joshua N. Leonard ,  Michelle E. Hung

    IPC分类号： C07K14/705 ,  A61K31/713 ,  C12N15/88 ,  C12N15/85

    摘要： Disclosed are exosomes that include a packaging protein and a cargo RNA in which the packaging protein binds specifically to the cargo RNA. The packaging protein is a fusion protein that includes an RNA-binding domain and an exosome-targeting domain. The cargo RNA includes an RNA-motif that the RNA-binding domain of the fusion protein binds specifically such that the cargo RNA is packaged in the lumen of the exosomes.

    Engineered red blood cell-based biosensors
    6.
    发明授权
    Engineered red blood cell-based biosensors 有权

    公开(公告)号：US10753930B2

    公开(公告)日：2020-08-25

    申请号：US15908077

    申请日：2018-02-28

    申请人： Northwestern University

    发明人： Joshua N. Leonard ,  Kelly A. Schwarz ,  Taylor B. Dolberg

    IPC分类号： G01N33/555 ,  G01N33/566 ,  G01N21/64 ,  C12N5/078 ,  G01N33/80

    摘要： Disclosed are systems and methods for detecting extracellular ligands. The disclosed systems and method for detecting extracellular ligands typically comprise or utilize engineered red blood cells (eRBCs) that comprises modular extracellular sensors. The eRBCs may comprise: (i) a first exogenous extracellular sensor; the first extracellular sensor comprising: a) a ligand binding domain, b) a transmembrane domain, and c) a first fragment of a functional protein, and (ii) a second exogenous extracellular sensor; the second extracellular sensor comprising: a) a ligand binding domain, b) a transmembrane domain, and c) a second fragment of the functional protein. In the eRBCs, the ligand binding domain of the first exogenous sensor and the ligand binding domain of the second exogenous sensor bind to the same ligand to form a ternary complex, and the first fragment of the functional protein and the second fragment of the functional protein interact in the ternary complex to reconstitute functional activity of the functional protein.

    MODULAR EXTRACELLULAR SENSOR ARCHITECTURE FOR REGULATING GENES
    7.
    发明申请
    MODULAR EXTRACELLULAR SENSOR ARCHITECTURE FOR REGULATING GENES 审中-公开

    公开(公告)号：US20190338262A1

    公开(公告)日：2019-11-07

    申请号：US16343987

    申请日：2017-10-24

    申请人： Northwestern University

    发明人： Joshua N. Leonard ,  Kelly A. Schwarz

    IPC分类号： C12N9/22 ,  C12N15/11

    摘要： Disclosed are systems, components of systems, and methods for sensing extracellular ligands and/or modulating expression of an endogenous or exogenous gene in a cell. In some embodiments, the disclosed systems and methods comprise or utilize first and second exogenous extracellular sensors and/or nucleic acid sequences encoding the first and second exogenous extracellular sensors, wherein the first exogenous extracellular sensor comprises: a) a ligand binding domain; b) a transmembrane domain; c) a protease cleavage site; and d) a functional domain comprising an RNA-binding subdomain fused to a transcription regulatory subdomain; and the second exogenous extracellular sensor comprises: e) a ligand binding domain, f) a transmembrane domain, and g) a protease domain that cleaves the protease cleavage site of the first exogenous extracellular receptor.