公开(公告)号：US10954469B2

公开(公告)日：2021-03-23

申请号：US16423748

申请日：2019-05-28

申请人： Novozymes A/S

发明人： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC分类号： A61K38/44 ,  C11B3/00 ,  C12N9/16 ,  C12P7/64 ,  C12N9/20

摘要： The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20210171860A1

公开(公告)日：2021-06-10

申请号：US17177349

申请日：2021-02-17

申请人： Novozymes A/S

发明人： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC分类号： C11B3/00 ,  C12N9/16 ,  C12P7/64 ,  C12N9/20

摘要： The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20200010778A1

公开(公告)日：2020-01-09

申请号：US16491093

申请日：2018-03-19

申请人： Novozymes A/S

发明人： Christian Hans Holm ,  Per Munk Nielsen ,  Fanny Longin ,  Sara Maria Landvik ,  Jesper Brask ,  Kim Borch ,  Robert Piotr Olinski ,  Allan Noergaard ,  Hanna Maria Lilbaek ,  Marianne Linde Damstrup ,  Tianqi Sun ,  Ming Li

IPC分类号： C11B3/00 ,  C12N9/18

摘要： Provided herein is about refining of vegetable oil. Further provided is the processes in which phospholipids present in the vegetable oil are hydrolysed and the oil is subsequently subject to chemical refining.

公开(公告)号：US20160289658A1

公开(公告)日：2016-10-06

申请号：US14777617

申请日：2014-03-21

申请人： NOVOZYMES A/S

发明人： Kim Borch ,  Sara Landvik ,  Marianne Linde Damstrup ,  Jesper Brask

IPC分类号： C12N9/20 ,  C11B3/00

CPC分类号： C12N9/20 ,  C11B3/003 ,  C12N9/18 ,  C12Y301/01

摘要： The present invention relates to isolated polypeptides having phospholipase A activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

摘要翻译： 本发明涉及具有磷脂酶A活性的分离多肽和编码该多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US11851630B2

公开(公告)日：2023-12-26

申请号：US17177349

申请日：2021-02-17

申请人： Novozymes A/S

发明人： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC分类号： C12N9/16 ,  C11B3/00 ,  C12P7/6445 ,  C12N9/20

CPC分类号： C11B3/003 ,  C12N9/16 ,  C12N9/20 ,  C12P7/6445 ,  C12Y301/04003 ,  C12Y301/04011

摘要： The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20190284503A1

公开(公告)日：2019-09-19

申请号：US16423748

申请日：2019-05-28

申请人： Novozymes A/S

发明人： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC分类号： C11B3/00 ,  C12P7/64 ,  C12N9/20 ,  C12N9/16

摘要： The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US10351795B2

公开(公告)日：2019-07-16

申请号：US15126098

申请日：2015-03-19

申请人： Novozymes A/S

发明人： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC分类号： C12N9/20 ,  C11B3/00 ,  C12N9/16 ,  C12P7/64

摘要： The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20140303389A1

公开(公告)日：2014-10-09

申请号：US14358349

申请日：2012-11-20

申请人： ARCHER DANIELS MIDLAND COMPANY ,  NOVOZYMES A/S ,  FRITO - LAY NORTH AMERICA, INC.

发明人： Thomas George Crosby ,  Marianne Linde Damstrup ,  John Inmok Lee ,  Per Munk Nielson ,  Craig Jordan Weitz

IPC分类号： C12P7/64

CPC分类号： C12P7/6454 ,  C11C1/10 ,  C11C3/02

摘要： A process for producing a food oil containing at least 50% monounsaturated fatty acids from palm oil is disclosed. Fatty acids are released from palm oil glycerides, such as by fat-splitting. The free fatty acids (FFA) are separated to obtain a fraction enriched in unsaturated palm fatty acid. This fraction is subject to a condensation reaction with glycerol to form an oil comprising mainly triglycerides (triacylglycerols). The condensation reaction is catalyzed by an enzyme.

摘要翻译： 公开了一种从棕榈油中生产含有至少50％单不饱和脂肪酸的食用油的方法。 脂肪酸从棕榈油甘油酯中释放，例如通过脂肪分解。 分离游离脂肪酸（FFA）以获得富含不饱和棕榈脂肪酸的级​​分。 该馏分与甘油发生缩合反应以形成主要包含甘油三酯（三酰基甘油）的油。 缩合反应由酶催化。

公开(公告)号：US20240158718A1

公开(公告)日：2024-05-16

申请号：US18504863

申请日：2023-11-08

申请人： Novozymes A/S

发明人： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC分类号： C11B3/00 ,  C12N9/16 ,  C12N9/20 ,  C12P7/6445

CPC分类号： C11B3/003 ,  C12N9/16 ,  C12N9/20 ,  C12P7/6445 ,  C12Y301/04003 ,  C12Y301/04011

摘要： The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20230332069A1

公开(公告)日：2023-10-19

申请号：US18055373

申请日：2022-11-14

申请人： Novozymes A/S

发明人： Christian Hans Holm ,  Per Munk Nielsen ,  Fanny Longin ,  Sara Maria Landvik ,  Jesper Brask ,  Kim Borch ,  Robert Piotr Olinski ,  Allan Noergaard ,  Hanna Maria Lilbaek ,  Marianne Linde Damstrup ,  Tianqi Sun ,  Ming Li

IPC分类号： C11B3/00 ,  C12N9/18

CPC分类号： C11B3/003 ,  C12N9/18

摘要： The present invention relates to refining of vegetable oil. The invention provides processes in which phospholipids present in the vegetable oil are hydrolysed and the oil is subsequently subject to chemical refining.