公开(公告)号：US20150276735A1

公开(公告)日：2015-10-01

申请号：US14667206

申请日：2015-03-24

申请人： OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES

发明人： Peiwu Li ,  Ting He ,  Qi Zhang ,  Zhaowei Zhang ,  Xiaoxia Ding

IPC分类号： G01N33/569 ,  C07K16/14

CPC分类号： G01N33/56961 ,  C07K16/14 ,  C07K2317/565 ,  C07K2317/569 ,  G01N2333/38 ,  G01N2469/10

摘要： Aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, and is encoded by the gene sequence of SEQ ID NO:8. The aflatoxin M1 nanobody 2014AFM-G2 obtained via screening has the properties of tolerance to organic reagents, tolerance to high temperature, tolerance to acids and bases and the like, and good stability. The aflatoxin M1 nanobody 2014AFM-G2 has 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxin B1, B2, G1, G2 are 9.43%, 5.93%, 4.87% and 6.17%, respectively.

摘要翻译： 黄曲霉毒素M1纳米颗粒2014AFM-G2具有SEQ ID NO：7的氨基酸序列，由SEQ ID NO：8的基因序列编码。 通过筛选获得的黄曲霉毒素M1纳米粒子2014AFM-G2具有对有机试剂的耐受性，耐高温性，耐酸碱性等优良的稳定性。 黄曲霉毒素M1纳米粒子2014AFM-G2对黄曲霉毒素M1的IC50值为0.208 ng / mL，具有50％的黄曲霉毒素B1，B2，G1，G2的交叉反应率分别为9.43％，5.93％，4.87％和6.17％ 。

公开(公告)号：US20150276729A1

公开(公告)日：2015-10-01

申请号：US14667378

申请日：2015-03-24

申请人： OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES

发明人： Peiwu Li ,  Qi Zhang ,  Ting He ,  Zhaowei Zhang ,  Xiaoxia Ding

IPC分类号： G01N33/543 ,  B01J20/281

CPC分类号： G01N30/48 ,  G01N33/54346 ,  G01N2030/486

摘要： An aflatoxin M1 nanobody, an immunosorbent and an immunoaffinity column. The aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, is encoded by the nucleic acid sequence of SEQ IDNO:8, has a 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxins B1, B2, G1, and G2 of 9.43%, 5.93%, 4.87% and 6.17%, respectively. The immunosorbent includes a solid phase carrier and aflatoxin M1 nanobody 2014AFM-G2 coupled with the solid phase carrier. The immunoaffinity column is loaded with the aflatoxin M1 nanobody immunosorbent. It can be used for purifying and concentrating an extracting solution of a sample before loading to a machine for detection and the immunoaffinity column can be used repeatedly for many times.

摘要翻译： 黄曲霉毒素M1纳米体，免疫吸附剂和免疫亲和柱。 黄曲霉毒素M1纳米粒子2014AFM-G2具有SEQ ID NO：7的氨基酸序列，由SEQ ID NO：8的核酸序列编码，对黄曲霉毒素M1的IC50值为0.208ng / mL，具有50％的抑制浓度，具有 黄曲霉毒素B1，B2，G1和G2的交叉反应率分别为9.43％，5.93％，4.87％和6.17％。 免疫吸附剂包括与固相载体偶联的固相载体和黄曲霉毒素M1纳米颗粒。 免疫亲和柱装载有黄曲霉毒素M1纳米体免疫吸附剂。 它可以用于在加载到机器进行检测之前将样品的提取溶液进行纯化和浓缩，并且免疫亲和柱可以重复使用多次。

公开(公告)号：US09128089B1

公开(公告)日：2015-09-08

申请号：US14667206

申请日：2015-03-24

申请人： OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES

发明人： Peiwu Li ,  Ting He ,  Qi Zhang ,  Zhaowei Zhang ,  Xiaoxia Ding

IPC分类号： G01N33/53 ,  A61K51/10 ,  G01N33/569 ,  C07K16/14

CPC分类号： G01N33/56961 ,  C07K16/14 ,  C07K2317/565 ,  C07K2317/569 ,  G01N2333/38 ,  G01N2469/10

摘要： Aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, and is encoded by the gene sequence of SEQ ID NO:8. The aflatoxin M1 nanobody 2014AFM-G2 obtained via screening has the properties of tolerance to organic reagents, tolerance to high temperature, tolerance to acids and bases and the like, and good stability. The aflatoxin M1 nanobody 2014AFM-G2 has 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxin B1, B2, G1, G2 are 9.43%, 5.93%, 4.87% and 6.17%, respectively.

摘要翻译： 黄曲霉毒素M1纳米颗粒2014AFM-G2具有SEQ ID NO：7的氨基酸序列，由SEQ ID NO：8的基因序列编码。 通过筛选获得的黄曲霉毒素M1纳米粒子2014AFM-G2具有对有机试剂的耐受性，耐高温性，耐酸碱性等优良的稳定性。 黄曲霉毒素M1纳米粒子2014AFM-G2对黄曲霉毒素M1的IC50值为0.208 ng / mL，具有50％的黄曲霉毒素B1，B2，G1，G2的交叉反应率分别为9.43％，5.93％，4.87％和6.17％ 。

公开(公告)号：US09435776B2

公开(公告)日：2016-09-06

申请号：US14667378

申请日：2015-03-24

申请人： OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES

发明人： Peiwu Li ,  Qi Zhang ,  Ting He ,  Zhaowei Zhang ,  Xiaoxia Ding

IPC分类号： G01N33/543 ,  G01N1/40 ,  G01N30/02 ,  A61K51/10 ,  B01J20/281 ,  G01N30/00

CPC分类号： G01N30/48 ,  G01N33/54346 ,  G01N2030/486

摘要： An aflatoxin M1 nanobody, an immunosorbent and an immunoaffinity column. The aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, is encoded by the nucleic acid sequence of SEQ ID NO:8, has a 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxins B1, B2, G1, and G2 of 9.43%, 5.93%, 4.87% and 6.17%, respectively. The immunosorbent includes a solid phase carrier and aflatoxin M1 nanobody 2014AFM-G2 coupled with the solid phase carrier. The immunoaffinity column is loaded with the aflatoxin M1 nanobody immunosorbent. It can be used for purifying and concentrating an extracting solution of a sample before loading to a machine for detection and the immunoaffinity column can be used repeatedly for many times.

摘要翻译： 黄曲霉毒素M1纳米体，免疫吸附剂和免疫亲和柱。 黄曲霉毒素M1纳米粒子2014AFM-G2具有由SEQ ID NO：8的核酸序列编码的SEQ ID NO：7的氨基酸序列，对黄曲霉毒素M1的IC50抑制率为0.208ng / mL，抑制浓度为50％， 黄曲霉毒素B1，B2，G1，G2的交叉反应率分别为9.43％，5.93％，4.87％和6.17％。 免疫吸附剂包括与固相载体偶联的固相载体和黄曲霉毒素M1纳米颗粒。 免疫亲和柱装载有黄曲霉毒素M1纳米体免疫吸附剂。 它可以用于在加载到机器进行检测之前将样品的提取溶液进行纯化和浓缩，并且免疫亲和柱可以重复使用多次。