公开(公告)号：US20170284976A1

公开(公告)日：2017-10-05

申请号：US15464378

申请日：2017-03-21

Applicant: The Govt. of the U.S.A., as represented by the Secretary of the Navy

Inventor： R. Andrew McGill ,  Robert Furstenberg ,  Viet K. Nguyen ,  Chris Kendziora ,  Michael Papantonakis ,  Todd H. Stievater

IPC: G01N30/02 ,  G01N30/60 ,  G01N21/77 ,  G01N21/552 ,  G01N21/3504 ,  G01N21/03 ,  B01J20/281 ,  G01N21/39

CPC classification number: G01N30/02 ,  G01N21/0332 ,  G01N21/3504 ,  G01N21/39 ,  G01N21/552 ,  G01N21/658 ,  G01N21/7746 ,  G01N30/48 ,  G01N30/6034 ,  G01N30/6082 ,  G01N30/74 ,  G01N2021/4761 ,  G01N2030/025

Abstract: A chemical detector for rapid, simultaneous detection of multiple chemicals including chemical warfare agents, toxic industrial chemicals, and explosives having one or more gas chromatography columns each with a chemosorbent or a chemo-reactive stationary phase and an infrared-transparent base, a bright infrared light source, a mechanism to direct the light source to any point along any of the columns, and an infrared sensor. Another disclosed detector has one or more gas chromatography columns each on the surface of a substrate having at least one infrared-transparent waveguide pattern, a bright infrared light source, and at least one ring resonator for each column, where each ring resonator is coated with a chemosorbent or a chemo-reactive stationary phase, and where each ring resonator spectroscopically probes the stationary phase. Also disclosed are the related methods for chemical detection.

公开(公告)号：US20170108476A1

公开(公告)日：2017-04-20

申请号：US15251553

申请日：2016-08-30

Applicant: Sunghan JUNG ,  Ami CHOI ,  JungDae PARK ,  Dong-Soo LEE ,  Ji-Won EOM ,  Kyung-Soo CHAE ,  Kwang-Shin LIM

Inventor： Sunghan JUNG ,  Ami CHOI ,  JungDae PARK ,  Dong-Soo LEE ,  Ji-Won EOM ,  Kyung-Soo CHAE ,  Kwang-Shin LIM

IPC: G01N30/64 ,  B01J20/281 ,  G01N30/96

CPC classification number: G01N30/64 ,  G01N30/48 ,  G01N30/96

Abstract: A conductivity detector includes a flow channel, an electrode arrangement, and a detector. The flow channel has a tube shape with a channel diameter through which a solution including ion components flows. The electrode arrangement is on the flow channel and includes at least an anode and at least a cathode. The anode and cathode are spaced apart by an electrode gap less than or equal to the channel diameter. The detector is connected to the electrode arrangement to detect electrical conductivity of the ion components.

公开(公告)号：US09599567B2

公开(公告)日：2017-03-21

申请号：US14208088

申请日：2014-03-13

Applicant: R. Andrew McGill ,  Robert Furstenberg ,  Viet K. Nguyen ,  Chris Kendziora ,  Michael Papantonakis ,  Todd H. Stievater

Inventor： R. Andrew McGill ,  Robert Furstenberg ,  Viet K. Nguyen ,  Chris Kendziora ,  Michael Papantonakis ,  Todd H. Stievater

IPC: G01N30/72 ,  G01N21/77 ,  G01N30/74 ,  G01N21/552 ,  G01N30/60 ,  G01N21/3504 ,  G01N21/03 ,  G01N21/65

CPC classification number: G01N30/02 ,  G01N21/0332 ,  G01N21/3504 ,  G01N21/39 ,  G01N21/552 ,  G01N21/658 ,  G01N21/7746 ,  G01N30/48 ,  G01N30/6034 ,  G01N30/6082 ,  G01N30/74 ,  G01N2021/4761 ,  G01N2030/025

Abstract: A chemical detector for rapid, simultaneous detection of multiple chemicals including chemical warfare agents, toxic industrial chemicals, and explosives having one or more gas chromatography columns each with a chemosorbent or a chemo-reactive stationary phase and an infrared-transparent base, a bright infrared light source, a mechanism to direct the light source to any point along any of the columns, and an infrared sensor. Another disclosed detector has one or more gas chromatography columns each on the surface of a substrate having at least one infrared-transparent waveguide pattern, a bright infrared light source, and at least one ring resonator for each column, where each ring resonator is coated with a chemosorbent or a chemo-reactive stationary phase, and where each ring resonator spectroscopically probes the stationary phase. Also disclosed are the related methods for chemical detection.

公开(公告)号：US20160258910A1

公开(公告)日：2016-09-08

申请号：US14638544

申请日：2015-03-04

Applicant: Jonathan James Grandy ,  German Augusto Gomez-Rios ,  Janusz Boleslaw Pawliszyn

Inventor： Jonathan James Grandy ,  German Augusto Gomez-Rios ,  Janusz Boleslaw Pawliszyn

IPC: G01N30/04 ,  B01J20/281 ,  G01N1/40

CPC classification number: G01N30/04 ,  B01L2300/069 ,  G01N1/405 ,  G01N30/08 ,  G01N30/48 ,  G01N2001/2893 ,  G01N2030/009 ,  G01N2030/045 ,  G01N2030/062 ,  G01N2030/488

Abstract: The invention describes the process by which a standard mixture of organic compounds are spiked and retained onto a composite sorbent matrix for the controlled generation of a standard in fluid above the spiked matrix either in gas (headspace) or aqueous phase which is contained in portable vials. The novelty of the aforementioned composite matrix stems from the combination of an immobilizing liquid phase such as silicone oil or a polyacrylonitrile solution, and solid, porous particles such as polystyrene-co-divinylbenzene (PS-DVB) or Hydrophilic/Lipophilic Balance (HLB) particles to strongly retain the spiked standards facilitating high capacity which translates into prolonged use as a source of calibrant. These novel composite mixtures exhibit sorptive capabilities greater than the sum of their individual components producing a standard analyte generator far superior to those described in the prior art. In addition swelling of the particles with the liquid phase facilitates immobilization of the composite sorbent matrix in the vial. Immobilization of said particles has also been achieved by cross-linking of a liquid polymeric solution such as polydimethylsiloxane (PDMS), allowing the composite sorbent matrix to be uniformly distributed on the vessel wall. With thermodynamic equilibrium strongly favoring the sorbent phase for a wide range of chemical compounds, this invention allows for the reproducible generation of an ultra-low concentration standard analyte mixture in fluid. If the fluid is gaseous, extractions from this headspace can be performed via solid phase microextraction (SPME), needle trap devices (NTD), or direct headspace extraction for injection onto various hyphenated gas chromatography (GC) systems. If the fluid is aqueous, extractions may be performed directly from the standard water for injection onto hyphenated GC or liquid chromatography (LC) systems. The invention can be used for a variety of applications ranging from integrated GC-MS or LC-MS tuning, automated instrument quality control (QC), preparation of reusable external calibration mixtures and, addition of internal standards wherein the method provides long-term stability, inter-batch reproducibility, repeatable analyte loading of the fluid, and quantifiable low concentrations at given temperatures.

公开(公告)号：US20150276729A1

公开(公告)日：2015-10-01

申请号：US14667378

申请日：2015-03-24

Applicant: OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES

Inventor： Peiwu Li ,  Qi Zhang ,  Ting He ,  Zhaowei Zhang ,  Xiaoxia Ding

IPC: G01N33/543 ,  B01J20/281

CPC classification number: G01N30/48 ,  G01N33/54346 ,  G01N2030/486

Abstract: An aflatoxin M1 nanobody, an immunosorbent and an immunoaffinity column. The aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, is encoded by the nucleic acid sequence of SEQ IDNO:8, has a 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxins B1, B2, G1, and G2 of 9.43%, 5.93%, 4.87% and 6.17%, respectively. The immunosorbent includes a solid phase carrier and aflatoxin M1 nanobody 2014AFM-G2 coupled with the solid phase carrier. The immunoaffinity column is loaded with the aflatoxin M1 nanobody immunosorbent. It can be used for purifying and concentrating an extracting solution of a sample before loading to a machine for detection and the immunoaffinity column can be used repeatedly for many times.

Abstract translation: 黄曲霉毒素M1纳米体，免疫吸附剂和免疫亲和柱。 黄曲霉毒素M1纳米粒子2014AFM-G2具有SEQ ID NO：7的氨基酸序列，由SEQ ID NO：8的核酸序列编码，对黄曲霉毒素M1的IC50值为0.208ng / mL，具有50％的抑制浓度，具有 黄曲霉毒素B1，B2，G1和G2的交叉反应率分别为9.43％，5.93％，4.87％和6.17％。 免疫吸附剂包括与固相载体偶联的固相载体和黄曲霉毒素M1纳米颗粒。 免疫亲和柱装载有黄曲霉毒素M1纳米体免疫吸附剂。 它可以用于在加载到机器进行检测之前将样品的提取溶液进行纯化和浓缩，并且免疫亲和柱可以重复使用多次。

公开(公告)号：US09134306B2

公开(公告)日：2015-09-15

申请号：US14071494

申请日：2013-11-04

Applicant: Intrinsic Bioprobes, Inc.

Inventor： Kemmons Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

IPC: G01N30/60 ,  G01N33/543 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B82Y30/00 ,  G01N33/68 ,  G01N30/02

CPC classification number: G01N33/6845 ,  B01J20/28097 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6095 ,  G01N33/54366 ,  G01N33/545 ,  G01N33/6803 ,  G01N2570/00 ,  H01J49/164 ,  H01J49/40 ,  Y10T436/255 ,  B01D15/3804

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

公开(公告)号：US20140162369A1

公开(公告)日：2014-06-12

申请号：US14130112

申请日：2012-01-11

Applicant: Kazuyuki Oishi

Inventor： Kazuyuki Oishi

IPC: B01J20/281

CPC classification number: G01N30/48 ,  B01D15/362 ,  B01J20/285 ,  B01J39/26 ,  B01J2220/58 ,  G01N2030/027 ,  G01N2030/8822

Abstract: The present invention aims to provide a method of measuring hemoglobins which can measure hemoglobins in a short time at high accuracy. The present invention also aims to provide a method of measuring hemoglobin A1c, a method of simultaneously measuring hemoglobin A1c and hemoglobin F, a method of simultaneously measuring hemoglobin A1c and hemoglobin A2, and a method of simultaneously measuring hemoglobin A1c and abnormal hemoglobins, each utilizing the above-mentioned method of measuring hemoglobins by liquid chromatography. The present invention relates to a method of measuring hemoglobins by liquid chromatography. A column used in the method is filled with, as a column-packing material, cation-exchangeable particles including cross-linked polymer particles having a cation-exchange-group-containing polymer bonded to the surface of the cross-linked polymer particles, and the column shows a pressure value of 9.8×103 Pa or higher and 29.4×105 Pa or lower when an eluent for measurement is delivered at 1.0 mL/min.

Abstract translation: 本发明的目的在于提供一种能够高精度地测定短时间内的血红蛋白的血红蛋白的测定方法。 本发明的目的还在于提供一种测定血红蛋白A1c的方法，同时测定血红蛋白A1c和血红蛋白F的方法，同时测定血红蛋白A1c和血红蛋白A2的方法，以及同时测定血红蛋白A1c和异常血红蛋白的方法， 上述通过液相色谱法测定血红蛋白的方法。 本发明涉及通过液相色谱法测定血红蛋白的方法。 在该方法中使用的柱子作为柱状填充材料填充有可交联聚合物颗粒的阳离子交换颗粒，所述交联聚合物颗粒具有结合到交联聚合物颗粒表面的含阳离子交换基团的聚合物，以及 当以1.0mL / min的速度递送测量用洗脱液时，该柱的压力值为9.8×10 3 Pa以上，29.4×10 5 Pa以下。

公开(公告)号：US08574434B2

公开(公告)日：2013-11-05

申请号：US13764394

申请日：2013-02-11

Applicant: Intrinsic Bioprobes, Inc.

Inventor： Kemmons Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

IPC: B01D15/08

CPC classification number: G01N33/6845 ,  B01J20/28097 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6095 ,  G01N33/54366 ,  G01N33/545 ,  G01N33/6803 ,  G01N2570/00 ,  H01J49/164 ,  H01J49/40 ,  Y10T436/255 ,  B01D15/3804

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

公开(公告)号：US20110192778A1

公开(公告)日：2011-08-11

申请号：US13035942

申请日：2011-02-26

Applicant: Kemmons A. Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

Inventor： Kemmons A. Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

IPC: B01D15/08

CPC classification number: G01N33/6845 ,  B01J20/28097 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6095 ,  G01N33/54366 ,  G01N33/545 ,  G01N33/6803 ,  G01N2570/00 ,  H01J49/164 ,  H01J49/40 ,  Y10T436/255 ,  B01D15/3804

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

Abstract translation: 描述了一种亲和力微柱，其包含高表面积材料，其具有包含在壳体内的高流动性和低死体积，并且具有结合到高表面积材料的表面的亲和试剂，其被激活或可激活。 结合亲和微柱表面的亲和试剂还包含用于整合生物分子的高通量分析的亲和受体。

公开(公告)号：US20100181239A1

公开(公告)日：2010-07-22

申请号：US12643798

申请日：2009-12-21

Applicant: Kemmons A. Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

Inventor： Kemmons A. Tubbs ,  Karl F. Gruber ,  Randall W. Nelson

IPC: B01D15/08

CPC classification number: G01N33/6845 ,  B01J20/28097 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6095 ,  G01N33/54366 ,  G01N33/545 ,  G01N33/6803 ,  G01N2570/00 ,  H01J49/164 ,  H01J49/40 ,  Y10T436/255 ,  B01D15/3804

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

Abstract translation: 描述了一种亲和力微柱，其包含高表面积材料，其具有包含在壳体内的高流动性和低死体积，并且具有结合到高表面积材料的表面的亲和试剂，其被激活或可激活。 结合亲和微柱表面的亲和试剂还包含用于整合生物分子的高通量分析的亲和受体。