IMMUNOSORBENT AND IMMUNOAFFINITY COLUMN FOR AFLATOXIN M1 NANOBODY AND PREPARATION METHOD THEREOF
    3.
    发明申请
    IMMUNOSORBENT AND IMMUNOAFFINITY COLUMN FOR AFLATOXIN M1 NANOBODY AND PREPARATION METHOD THEREOF 有权
    AFLATOXIN M1 NANOBODY的免疫球蛋白和免疫球蛋白及其制备方法

    公开(公告)号:US20150276729A1

    公开(公告)日:2015-10-01

    申请号:US14667378

    申请日:2015-03-24

    IPC分类号: G01N33/543 B01J20/281

    摘要: An aflatoxin M1 nanobody, an immunosorbent and an immunoaffinity column. The aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, is encoded by the nucleic acid sequence of SEQ IDNO:8, has a 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxins B1, B2, G1, and G2 of 9.43%, 5.93%, 4.87% and 6.17%, respectively. The immunosorbent includes a solid phase carrier and aflatoxin M1 nanobody 2014AFM-G2 coupled with the solid phase carrier. The immunoaffinity column is loaded with the aflatoxin M1 nanobody immunosorbent. It can be used for purifying and concentrating an extracting solution of a sample before loading to a machine for detection and the immunoaffinity column can be used repeatedly for many times.

    摘要翻译: 黄曲霉毒素M1纳米体,免疫吸附剂和免疫亲和柱。 黄曲霉毒素M1纳米粒子2014AFM-G2具有SEQ ID NO:7的氨基酸序列,由SEQ ID NO:8的核酸序列编码,对黄曲霉毒素M1的IC50值为0.208ng / mL,具有50%的抑制浓度,具有 黄曲霉毒素B1,B2,G1和G2的交叉反应率分别为9.43%,5.93%,4.87%和6.17%。 免疫吸附剂包括与固相载体偶联的固相载体和黄曲霉毒素M1纳米颗粒。 免疫亲和柱装载有黄曲霉毒素M1纳米体免疫吸附剂。 它可以用于在加载到机器进行检测之前将样品的提取溶液进行纯化和浓缩,并且免疫亲和柱可以重复使用多次。

    UREAURETHANES FOR RHEOLOGY CONTROL
    5.
    发明申请

    公开(公告)号:US20180170860A1

    公开(公告)日:2018-06-21

    申请号:US15577970

    申请日:2016-07-22

    申请人: BYK-Chemie, GmbH

    摘要: The present invention relates to ureaurethanes of the following formula (I) in which at least one of the R1 or R2 radicals is a mono- or polyunsaturated, branched or unbranched alkenyl or alkynyl radical having 12 to 24 carbon atoms, n is an integer ≥1, where the upper limit for n arises from the maximum number average molecular weight Mn of the ureaurethanes of the general formula (I), which is 65 000 g/mol, and which is determined by means of gel permeation chromatography to DIN 55672-2 using a polymethyl methacrylate standard, R3 is a xylylene radical or a hydrogenated xylylene radical and R4 is a tolylene radical or various other radicals. The invention also relates to ureaurethane compositions comprising the ureaurethanes of the invention and to the preparation of both. The invention further relates to the use of the ureaurethanes or ureaurethane compositions as rheology control agent and anti-settling agent. The invention further provides liquid formulations from the group consisting of coating compositions, polymer formulations, pigment pastes, sealant formulations, cosmetics, ceramic formulations, drilling fluids, adhesive formulations, potting compounds, construction material formulations, lubricants, spackling compounds, printing inks and other inks, comprising the ureaurethanes and ureaurethane compositions.

    Integrated High-Throughput Methods to Characterize Multi-Component Polymers

    公开(公告)号:US20180059076A1

    公开(公告)日:2018-03-01

    申请号:US15658904

    申请日:2017-07-25

    发明人: Shuhui Kang

    摘要: A method of analyzing a multi-component polymer comprising: (a) dissolving an multi-component polymer having a primary monomer and primary comonomer to form a first volume (soluble portion of multi-component polymer); (b) injecting a portion of the first volume into a chromatographic column to get elution first slices, leaving a second volume behind; (c) filtering the second volume to isolate multi-component polymer solids; (d) dissolving solids to form solution third solution (insoluble portion of multi-component polymer); (e) injecting a portion of third solution into the chromatographic column to get elution second slices; (f) obtain infra-red spectra at wavelengths suitable for the primary monomer and the primary comonomer of first and second elution slices, separately; and (g) for each elution slice, separately calculate: (i) the different polymer components (soluble and insoluble); and (ii) the comonomer content of each component (soluble and insoluble).

    Immunosorbent and immunoaffinity column for aflatoxin M1 nanobody and preparation method thereof
    8.
    发明授权
    Immunosorbent and immunoaffinity column for aflatoxin M1 nanobody and preparation method thereof 有权
    免疫吸附剂和免疫亲和柱黄曲霉毒素M1纳米体及其制备方法

    公开(公告)号:US09435776B2

    公开(公告)日:2016-09-06

    申请号:US14667378

    申请日:2015-03-24

    摘要: An aflatoxin M1 nanobody, an immunosorbent and an immunoaffinity column. The aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, is encoded by the nucleic acid sequence of SEQ ID NO:8, has a 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxins B1, B2, G1, and G2 of 9.43%, 5.93%, 4.87% and 6.17%, respectively. The immunosorbent includes a solid phase carrier and aflatoxin M1 nanobody 2014AFM-G2 coupled with the solid phase carrier. The immunoaffinity column is loaded with the aflatoxin M1 nanobody immunosorbent. It can be used for purifying and concentrating an extracting solution of a sample before loading to a machine for detection and the immunoaffinity column can be used repeatedly for many times.

    摘要翻译: 黄曲霉毒素M1纳米体,免疫吸附剂和免疫亲和柱。 黄曲霉毒素M1纳米粒子2014AFM-G2具有由SEQ ID NO:8的核酸序列编码的SEQ ID NO:7的氨基酸序列,对黄曲霉毒素M1的IC50抑制率为0.208ng / mL,抑制浓度为50%, 黄曲霉毒素B1,B2,G1,G2的交叉反应率分别为9.43%,5.93%,4.87%和6.17%。 免疫吸附剂包括与固相载体偶联的固相载体和黄曲霉毒素M1纳米颗粒。 免疫亲和柱装载有黄曲霉毒素M1纳米体免疫吸附剂。 它可以用于在加载到机器进行检测之前将样品的提取溶液进行纯化和浓缩,并且免疫亲和柱可以重复使用多次。

    METHOD OF MANUFACTURING GRANULAR POLYARYLENE SULFIDE, AND GRANULAR POLYARYLENE SULFIDE

    公开(公告)号:US20180171078A1

    公开(公告)日:2018-06-21

    申请号:US15580074

    申请日:2016-06-10

    摘要: To provide: a method of manufacturing granular polyarylene sulfide (PAS) with improved particle strength while improving the yield of the granular PAS by introducing and recovering PAS with a moderate molecular weight in granular PAS; and granular PAS.A method of manufacturing granular PAS according to the present invention includes: a polymerizing step of subjecting a dihalo aromatic compound and at least one type of sulfur source selected from the group consisting of alkali metal sulfides and alkali metal hydrosulfides to polymerization reaction in an organic amide solvent; and a cooling step of cooling the reaction product mixture after the polymerizing step. Addition of a phase separation agent to the reaction product mixture is started at a point in time from the start of the polymerizing step to before the start of forming granular PAS in the cooling step, and when the temperature of the reaction product mixture is 245° C. or higher, 50 mass % or greater of the phase separation agent is added to the reaction product mixture. The polymerizing step includes a predetermined PAS prepolymer generating step, and a predetermined converting step to a high molecular weight PAS.