公开(公告)号：US10895728B2

公开(公告)日：2021-01-19

申请号：US16186303

申请日：2018-11-09

Applicant: OLYMPUS CORPORATION

Inventor： Yoshihiro Shimada ,  Kazuhito Goda

IPC: G02B21/00 ,  G02B21/02 ,  G02B21/33

Abstract: Provided is a sample observation method including: bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate; and collecting light from the observation target by means of an objective lens via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until the contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens of a microscope to pass therethrough.

公开(公告)号：US10914726B2

公开(公告)日：2021-02-09

申请号：US16181782

申请日：2018-11-06

Applicant: OLYMPUS CORPORATION

Inventor： Mayu Ogawa ,  Kazuhito Goda ,  Kosuke Takagi

IPC: G06K9/00 ,  G01N33/50 ,  G01N21/64 ,  G01N21/17

Abstract: The present invention provides a method for evaluating a protrusion-forming ability of cell spheroids, comprising step (a) of imaging a cell spheroid labeled with a fluorescent substance using a fluorescence microscope at a resolution capable of identifying individual cells, and acquiring a plane tomographic image of a fluorescence emitted from the fluorescent substance, step (b) of analyzing the plane tomographic image acquired in step (a) to determine a protrusion part of the cell spheroid, and step (c) of evaluating a protrusion-forming ability of the cell spheroid based on the protrusion part determined in step (b).