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公开(公告)号：US10168520B2
公开(公告)日：2019-01-01
申请号：US15161997
申请日：2016-05-23
Applicant: OLYMPUS CORPORATION
Inventor： Hisao Kitagawa , Yoshihiro Shimada , Kosuke Takagi
Abstract: Provided is a microscope system including a motor-driven stage on which is mounted a culture vessel containing one or more cell clusters, each including cells having a target molecule labeled with a fluorescent or luminescent chemical; a low-magnification-image acquiring unit that acquires low-magnification images of the cell clusters in the culture vessel mounted on the stage; a detecting unit that detects the position of each cell cluster in the culture vessel by analyzing the acquired low-magnification images; and a high-magnification-image acquiring unit that, after the detected position is aligned with the optical axis of an objective lens, acquires slice images of fluorescence or luminescence emitted from the cells forming the cell cluster at a higher magnification than the low-magnification-image acquiring unit at intervals along the optical axis while the stage and/or the lens is moved to change stepwise the distance between the lens and the cell cluster.
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公开(公告)号：US10114209B2
公开(公告)日：2018-10-30
申请号：US14559611
申请日：2014-12-03
Applicant: OLYMPUS CORPORATION
Inventor： Kosuke Takagi
Abstract: A plurality of specimens or a plurality of targets in a specimen are accurately examined. A microscope apparatus includes a light source; an image-generating unit generating an image of a specimen; a control unit controlling the light source and the image-generating unit depending on a predetermined irradiation condition or a predetermined image generation condition; an image-analyzing unit analyzing the image generated by the image-generating unit to extract targets; and a condition-changing unit changing the irradiation condition and/or the image generation condition based on a difference between an actual luminance of each of the extracted targets in the image and a desired luminance such that the actual luminance of the target in the image satisfies the desired luminance. If the irradiation condition and/or the image generation condition is changed, the control unit redrives the light source and the image-generating unit depending on the changed irradiation condition and/or image generation condition.
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公开(公告)号：US10914726B2
公开(公告)日：2021-02-09
申请号：US16181782
申请日：2018-11-06
Applicant: OLYMPUS CORPORATION
Inventor： Mayu Ogawa , Kazuhito Goda , Kosuke Takagi
Abstract: The present invention provides a method for evaluating a protrusion-forming ability of cell spheroids, comprising step (a) of imaging a cell spheroid labeled with a fluorescent substance using a fluorescence microscope at a resolution capable of identifying individual cells, and acquiring a plane tomographic image of a fluorescence emitted from the fluorescent substance, step (b) of analyzing the plane tomographic image acquired in step (a) to determine a protrusion part of the cell spheroid, and step (c) of evaluating a protrusion-forming ability of the cell spheroid based on the protrusion part determined in step (b).
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公开(公告)号：US09842393B2
公开(公告)日：2017-12-12
申请号：US15166195
申请日：2016-05-26
Applicant: OLYMPUS CORPORATION
Inventor： Kosuke Takagi , Yoshihiro Shimada
CPC classification number: G06T7/0012 , G01N33/4833 , G06K9/0014 , G06K9/52 , G06T7/66 , G06T7/70 , G06T2200/04 , G06T2207/30004 , G06T2207/30024
Abstract: A cell evaluation method includes: a labeling step of labeling at least some of cells constituting a cell clump with a chemical substance; an image-capturing step of acquiring a plurality of slice images of the labeled cells, for at least part of the cell clump; an evaluating step of evaluating the characteristics of the cells on the basis of the plurality of slice images; a 3D-image creating step of creating a 3D image of at least part of the cell clump by subjecting the plurality of slice images to image processing; a center-of-gravity determining step of determining, from the 3D image, a center-of-gravity position of the cell clump; and an organizing step of organizing evaluation results obtained in the evaluating step, with respect to the distance from the center-of-gravity position.
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