公开(公告)号：US20180120549A1

公开(公告)日：2018-05-03

申请号：US15728726

申请日：2017-10-10

Applicant: OLYMPUS CORPORATION

Inventor： Susumu HONDA

IPC: G02B21/00 ,  G02B27/28 ,  G02F1/01

CPC classification number: G02B21/0076 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/0068 ,  G02B27/283 ,  G02F1/0102

Abstract: A microscope apparatus includes: an objective lens; an observation optical system that makes the specimen be irradiated with excitation light by the objective lens, and acquires image information of the specimen; a first stimulation optical system that includes a scanning section which scans stimulus light, makes the scanning section scan an irradiation position of the stimulus light and applies an optical stimulus to the specimen; a second stimulation optical system that includes an SLM arranged at a pupil conjugate position of the objective lens and capable of modulating a phase of the stimulus light, selectively switches the irradiation position of the stimulus light by the SLM and applies the optical stimulus to the specimen; and a light path selecting section that selects at least one of a light path of the first stimulation optical system and a light path of the second stimulation optical system.

公开(公告)号：US20160161729A1

公开(公告)日：2016-06-09

申请号：US14957403

申请日：2015-12-02

Applicant: OLYMPUS CORPORATION

Inventor： Susumu HONDA

IPC: G02B21/00 ,  H01S3/00 ,  G02B21/24 ,  G02B27/14 ,  G02B21/16 ,  G02B21/02 ,  G02B27/00

CPC classification number: G02B21/0048 ,  G02B21/0032 ,  G02B21/0076

Abstract: An object is to enable LSM observation while correcting aberrations, as well as illumination with a desired pattern, using a single LCOS. An observation apparatus of the present invention includes an objective lens that focuses laser light emitted from a light source and radiates the laser light onto a specimen; an LCOS disposed at a position optically conjugate with the pupil position of the objective lens and capable of modulating, according to a wavefront modulation pattern, the wavefront of the laser light to be radiated onto the specimen by the objective lens; a scanner disposed at a position optically conjugate with the LCOS and capable of two-dimensionally scanning the laser light to be radiated onto the specimen by the objective lens; a focusing mechanism that adjusts the focal position, in the specimen, of the objective lens in the optical axis direction; and a control unit that controls the LCOS such that the LCOS corrects aberrations at the focal position adjusted by the focusing mechanism and forms a desired illumination pattern on the specimen.

Abstract translation: 目的是使用单个LCOS来校正像差和使用所需图案的照明来进行LSM观察。 本发明的观察装置包括对从光源射出的激光进行聚焦并将激光照射到试样上的物镜; LCOS设置在与物镜的光瞳位置光学共轭的位置处，并且能够根据波前调制图案调制由物镜辐射到样本上的激光的波前; 扫描器，其设置在与所述LCOS光学共轭的位置处，并且能够二维地扫描由所述物镜照射到所述样本上的激光; 调整物镜在光轴方向上的焦点位置的聚焦机构; 以及控制单元，其控制LCOS，使得LCOS校正由聚焦机构调整的焦点位置处的像差，并在样本上形成期望的照明图案。

公开(公告)号：US20170285315A1

公开(公告)日：2017-10-05

申请号：US15436742

申请日：2017-02-17

Applicant: OLYMPUS CORPORATION

Inventor： Yusuke AMANO ,  Susumu HONDA

IPC: G02B21/00 ,  G02B21/26 ,  G02B21/08

CPC classification number: G02B21/002 ,  G02B5/003 ,  G02B21/0088 ,  G02B21/08 ,  G02B21/16 ,  G02B21/26 ,  G02B27/0018

Abstract: Provided is a laser scanning microscope including a stage on which a sample is placed, an objective lens that is disposed below the stage and that focuses laser light from a light source onto the sample, a scanner that scans the laser light focused by the objective lens over the sample, a condenser lens disposed opposite the objective lens with the stage interposed therebetween, and a light blocking cover that is disposed in an optical path between the condenser lens and the stage and that blocks external light entering the objective lens or the condenser lens from above the sample via the stage.

公开(公告)号：US20140186856A1

公开(公告)日：2014-07-03

申请号：US14201418

申请日：2014-03-07

Applicant: OLYMPUS CORPORATION

Inventor： Susumu HONDA

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N2015/1472 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G02B21/16 ,  G02B21/367

Abstract: An observation apparatus and an observation method are provided. The observation apparatus includes a light source that emits illumination light used to observe a specimen to which a fluorescent substance that specifically binds to or is expressed in a stimulus target has been supplied, an illumination optical system that radiates the illumination light emitted from the light source 11 onto the specimen, a deflecting device that changes an area of the specimen to be irradiated with the illumination light, a wavelength selecting section that selects the wavelength of illumination light to be radiated onto the specimen, an observation optical system that collects light from the specimen, a detector that detects the light collected by the observation optical system, an image processing section that generates an image from the light detected by the detector, and a control section that controls these components.

Abstract translation: 提供观察装置和观察方法。 观察装置包括：发光用于观察已经提供了与刺激对象特异性结合或表达的荧光物质的样本的照明光的光源，照射光学系统，其照射从光源发出的照明光 11，将用照明光照射的样本的面积变化的偏转装置，选择要照射到样本上的照明光的波长的波长选择部，将来自所述照射光的照射光照射的观察光学系统 样本，检测由观察光学系统收集的光的检测器，根据由检测器检测到的光产生图像的图像处理部，以及控制这些成分的控制部。

公开(公告)号：US20130301121A1

公开(公告)日：2013-11-14

申请号：US13868885

申请日：2013-04-23

Applicant: OLYMPUS CORPORATION

Inventor： Susumu HONDA

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G02B21/002 ,  G02B21/0024 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/0088 ,  G02B21/24 ,  G02B26/008

Abstract: A microscope for observing a specimen, while switching optical-path splitting portions, by using bright images without a positional displacement between the images, includes an optical-axis moving portion that parallelly moves laser light; a scanning portion; an objective lens; a detector; a plurality of excitation dichroic mirrors placed in an optical path in an insertable/removable manner and split the optical path; an excitation DM turret that selectively switches the excitation dichroic mirrors; a storage portion storing entry-angle displacement information and transmitting-position displacement information for the laser light at the pupil position of the objective lens, which are associated with the individual excitation dichroic mirrors; and a control portion controlling the scanning portion based on the entry-angle displacement information associated with an excitation dichroic mirror placed in the optical path and also controlling the optical-axis moving portion 15 based on the transmitting-position displacement information associated with that dichroic mirror.

Abstract translation: 通过使用没有图像之间的位置偏移的明亮图像来切换光路分割部分的显微镜包括平行移动激光的光轴移动部分; 扫描部分; 物镜; 检测器 以可插入/拆卸的方式放置在光路中的多个激励二向色镜，并分裂光路; 激励DM转塔，其选择性地切换激发二向色镜; 存储部，其存储与各个激励分色镜相关联的物镜的光瞳位置处的入射角位移信息和用于激光的透射位置位移信息; 以及控制部，其基于与放置在光路中的激励分色镜相关联的入射角位移信息来控制扫描部，并且还基于与该分色镜相关联的透射位置位移信息来控制光轴移动部15 。