公开(公告)号：US20170268042A1

公开(公告)日：2017-09-21

申请号：US15505480

申请日：2015-08-24

Applicant: OSAKA UNIVERSITY

Inventor： Kazuhiko NAKATANI ,  Fumie SAKAMOTO

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6853

Abstract: Provided are a PCR method and a PCR kit each of which has higher detection accuracy and is more convenient. A PCR method of the present invention subjects a sample to a PCR reaction, the sample containing: a primer set including a first primer and a second primer; a template amplified by the primer set; a first probe which loses at least one bulge structure in a case where a double strand is formed by the first probe and the first primer and which forms the at least one bulge structure by being dissociated from the double strand formed by the first probe and the first primer; and a bulge structure-binding molecule which emits a signal by binding to the at least one bulge structure.