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1.发明授权公开(公告)号：US11834697B2
公开(公告)日：2023-12-05
申请号：US16644539
申请日：2018-09-17
发明人： Richard Guy Compton , Sabine Kuss
IPC分类号： C12Q1/26 , C12Q1/02 , G01N27/327 , G01N27/416 , G01N27/48
CPC分类号： C12Q1/26 , C12Q1/025 , G01N27/3277 , G01N27/4161 , G01N27/48 , G01N2333/90216
摘要： A method of determining the presence of bacteria expressing cytochrome c oxidase (‘the bacteria’), the method comprising: —providing a sample suspected of containing the bacteria; —providing a compound that has two redox states: a reduced state and an oxidised state, wherein cytochrome c oxidase can convert the compound from its reduced state to its oxidised state; —contacting an electrode either with (i) the compound in its oxidised state in the presence of the sample, then applying a reductive potential and measuring the current at the electrode; or (ii) the compound in its reduced state in the presence of the sample, then applying an oxidative potential and measuring the current at the electrode; and—comparing the magnitude of the current produced by the reductive potential or oxidative potential in the presence of the sample suspected of containing the bacteria with the magnitude of the current produced under the same conditions, but in the absence of the sample suspected of containing the bacteria, wherein a difference between the magnitude of current produced in the presence of the sample suspected of containing the bacteria and the magnitude of current produced in the absence of the sample suspected of containing the bacteria indicates the presence of the bacteria. Also provided herein is a sensor for determining the presence of bacteria expressing cytochrome c oxidase.
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公开(公告)号：US20170138884A1
公开(公告)日：2017-05-18
申请号：US15127315
申请日：2015-03-20
CPC分类号： G01N27/308 , G01N27/48 , G01N33/6815
摘要： Herein is disclosed a method for detecting a material comprising a thiol group (e.g. homocysteine, cysteine, glutathione) in a sample, the method comprising: •providing an electrode comprising a carbon material; •contacting said electrode with said sample in the presence of catechol or more generally a precursor of ortho-quinone; •performing cyclic or square wave voltammetry to convert catechol to ortho-quinone and wherein said ortho-quinone reacts with the material comprising a thiol group to form an electrochemical reaction product.
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公开(公告)号：US10928350B2
公开(公告)日：2021-02-23
申请号：US15127315
申请日：2015-03-20
摘要： Herein is disclosed a method for detecting a material comprising a thiol group (e.g. homocysteine, cysteine, glutathione) in a sample, the method comprising: •providing an electrode comprising a carbon material; •contacting said electrode with said sample in the presence of catechol or more generally a precursor of ortho-quinone; •performing cyclic or square wave voltammetry to convert catechol to ortho-quinone and wherein said ortho-quinone reacts with the material comprising a thiol group to form an electrochemical reaction product.
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