公开(公告)号：US20180201917A1

公开(公告)日：2018-07-19

申请号：US15922301

申请日：2018-03-15

申请人： Omrix Biopharmaceticals Ltd.

发明人： Tamar AUERBACH-NEVO ,  Nadav ORR ,  Israel NUR

IPC分类号： C12N9/74 ,  C07K14/745 ,  C12Q1/56 ,  G01N33/49 ,  A61K38/00

CPC分类号： C12N9/6429 ,  A61K38/00 ,  C07K14/745 ,  C12Q1/56 ,  C12Y304/21005 ,  G01N33/491 ,  G01N2333/96463

摘要： Provided is a method for purifying α-thrombin and for quantifying α-thrombin and its degradation polypeptides in a liquid proteinatious solution. The method employs a one-step anion exchange chromatography method. The method allows purification and/or quantification of a homogenous post-translationally modified α-thrombin. The method can also be used for purification and/or quantification of β-thrombin.