公开(公告)号：US20090317833A1

公开(公告)日：2009-12-24

申请号：US12225721

申请日：2007-03-29

申请人： Osamu Hazeki ,  Kaoru Hazeki ,  Masayuki Ii ,  Naoko Matsunaga

发明人： Osamu Hazeki ,  Kaoru Hazeki ,  Masayuki Ii ,  Naoko Matsunaga

IPC分类号： G01N33/53

CPC分类号： G01N33/566 ,  G01N2333/70596 ,  G01N2500/04 ,  G01N2500/10

摘要： The present invention provides a method of screening for a drug for the prophylaxis or treatment of at least one disease selected from the group consisting of cardiac disease, autoimmune disease, inflammatory disease, central nervous system disease, infectious disease, sepsis, severe sepsis and septic shock, which includes selecting a substance that binds to an intracellular region of TLR4, and inhibits signal transduction from said molecule, and a kit for this method, which contains (1) a cell that expresses wild-type TLR4 and (2) a cell that expresses a mutant TLR4, and which can detect a signal from TLR4 with expression of a reporter gene as an index.

摘要翻译： 本发明提供了一种筛选用于预防或治疗选自心脏病，自身免疫疾病，炎性疾病，中枢神经系统疾病，感染性疾病，败血症，严重败血症和败血症的至少一种疾病的药物的方法 其包括选择与TLR4的细胞内区域结合的物质，并抑制所述分子的信号转导，以及用于该方法的试剂盒，其包含（1）表达野生型TLR4的细胞和（2）细胞 其表达突变体TLR4，并且可以以报告基因的表达作为指标检测来自TLR4的信号。

公开(公告)号：US08329420B2

公开(公告)日：2012-12-11

申请号：US12225721

申请日：2007-03-29

申请人： Osamu Hazeki ,  Kaoru Hazeki ,  Masayuki Ii ,  Naoko Matsunaga

发明人： Osamu Hazeki ,  Kaoru Hazeki ,  Masayuki Ii ,  Naoko Matsunaga

IPC分类号： G01N33/567 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  C12N5/16 ,  C12N5/18 ,  C12N5/22 ,  C07K14/705

CPC分类号： G01N33/566 ,  G01N2333/70596 ,  G01N2500/04 ,  G01N2500/10

摘要： The present invention provides a method of screening for a drug for the prophylaxis or treatment of at least one disease selected from the group consisting of cardiac disease, autoimmune disease, inflammatory disease, central nervous system disease, infectious disease, sepsis, severe sepsis and septic shock, which includes selecting a substance that binds to an intracellular region of TLR4, and inhibits signal transduction from said molecule, and a kit for this method, which contains (1) a cell that expresses wild-type TLR4 and (2) a cell that expresses a mutant TLR4, and which can detect a signal from TLR4 with expression of a reporter gene as an index.

摘要翻译： 本发明提供了一种筛选用于预防或治疗选自心脏病，自身免疫疾病，炎性疾病，中枢神经系统疾病，感染性疾病，败血症，严重败血症和败血症的至少一种疾病的药物的方法 其包括选择与TLR4的细胞内区域结合的物质，并抑制所述分子的信号转导，以及用于该方法的试剂盒，其包含（1）表达野生型TLR4的细胞和（2）细胞 其表达突变体TLR4，并且可以以报告基因的表达作为指标检测来自TLR4的信号。