公开(公告)号：US10233229B2

公开(公告)日：2019-03-19

申请号：US14650188

申请日：2013-11-25

Applicant: Pfizer Inc.

Inventor： Tetsuya Ishino ,  Weili Duan ,  Ronald William Kriz

IPC: C07K1/00 ,  C07K16/00 ,  C12P21/08 ,  C07H21/04 ,  C12P21/06 ,  C12P21/04 ,  C12N5/00

Abstract: The present invention relates to monomeric polypeptides comprising an engineered monomeric antibody fragment (e.g., monomeric Fc-containing polypeptides) wherein the monomeric Fc comprises one or more engineered N-linked glycosylation sites in the CH3-CH3 dimerization interface. Methods for producing such engineered monomeric antibody fragments and their use in diagnostics and therapeutics are also provided.

公开(公告)号：US20150322135A1

公开(公告)日：2015-11-12

申请号：US14650188

申请日：2013-11-25

Applicant: PFIZER INC.

Inventor： Tetsuya Ishino ,  Weili Duan ,  Ronald William Kriz

IPC: C07K16/00

CPC classification number: C07K16/00 ,  C07K2299/00 ,  C07K2317/41 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/55 ,  C07K2317/64 ,  C07K2317/94

Abstract: The present invention relates to monomeric polypeptides comprising an engineered monomeric antibody fragment (e.g., monomeric Fc-containing polypeptides) wherein the monomeric Fc comprises one or more engineered N-linked glycosylation sites in the CH3-CH3 dimerization interface. Methods for producing such engineered monomeric antibody fragments and their use in diagnostics and therapeutics are also provided.

Abstract translation: 本发明涉及包含工程化单体抗体片段（例如含单体Fc的多肽）的单体多肽，其中单体Fc包含在CH3-CH3二聚化界面中的一个或多个工程化的N-连接的糖基化位点。 还提供了生产这种工程化单体抗体片段的方法及其在诊断和治疗中的应用。