公开(公告)号：US11246477B2

公开(公告)日：2022-02-15

申请号：US16289555

申请日：2019-02-28

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION ,  THE ASAN FOUNDATION ,  UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION ,  OSONG MEDICAL INNOVATION FOUNDATION

Inventor： Seong Han Kim ,  Seunghun Lee ,  Hoon Cheol Jang ,  Bumju Kim ,  Viet Hoan Le ,  Wonyeong Park ,  Ki Hean Kim ,  Seung Jae Myung ,  Seungrag Lee

IPC: A61B1/04 ,  A61B5/00 ,  A61K31/496

Abstract: A fluorescence endoscopic method for visualization of colorectal tissue using a fluoroquinolone antibiotic and a method for diagnosis of lesions of colorectal tissue using the same. The fluorescence endoscopic method for visualization of colorectal tissue includes staining goblet cells of the colorectal tissue with moxifloxacin, which is a fluoroquinolone antibiotic, and exciting the stained goblet cells with single photons in the near UV region or in the visible region, followed by fluorescence endoscopic photographing of the goblet cells, thereby enabling acquisition of morphological information on living tissue without damage to or destruction of the colorectal tissue, while allowing diagnosis of lesions of the colon based on the morphological information on living tissue such as information on the distribution of the goblet cells. Specifically, the fluorescence endoscopic method includes: a colorectal tissue staining step in which the crypt of the colorectal tissue is stained with a fluoroquinolone antibiotic; an image pickup unit insertion step in which an image pickup unit is inserted into the colon; a light irradiation step in which the colorectal tissue is irradiated with excitation light from a light source of the image pickup unit; a colorectal tissue photographing step in which the colorectal tissue is photographed using a photographing device of the image pickup unit through the fluoroquinolone antibiotic fluorescence-excited by light in the light irradiation step, wherein, in the colorectal tissue staining step, goblet cells of the crypt are stained with the fluoroquinolone antibiotic; in the light irradiation step, the light source emits single photons as excitation light inducing fluorescence of the fluoroquinolone antibiotic, the excitation light from the light source having a wavelength band within the near UV and visible regions; and, in the image pickup unit insertion step, the image pickup unit is a general fluorescence endoscope or a confocal endoscopic microscope.

公开(公告)号：US10859498B2

公开(公告)日：2020-12-08

申请号：US16289536

申请日：2019-02-28

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Seonghan Kim ,  Ki Hean Kim ,  Myoung Joon Kim ,  Seunghun Lee ,  Hoon Cheol Jang ,  Viet Hoan Le ,  Soo Hyun Park

IPC: G01N21/64 ,  G01N33/58 ,  G01N33/50

Abstract: A method for visualization of conjunctival cells using fluoroquinolone antibiotics and a method for diagnosis of ocular lesions using the same. The method for visualization of conjunctival cells using fluoroquinolone antibiotics includes staining goblet cells of ocular conjunctiva with moxifloxacin, which is a fluoroquinolone antibiotic, and exciting the stained goblet cells with single photons in the near-UV region or in the visible region, followed by fluorescence photographing of the goblet cells, thereby enabling acquisition of morphological information on living tissue without damage to or destruction of the ocular conjunctiva. Specifically, the method for visualization of conjunctival cells includes: a conjunctiva staining step in which ocular conjunctiva is stained with a fluoroquinolone antibiotic; a light irradiation step in which the ocular conjunctiva stained with the fluoroquinolone antibiotic is irradiated with light from a light source; and a conjunctiva photographing step in which the ocular conjunctiva is photographed using an image pickup unit through the fluoroquinolone antibiotic fluorescence-excited by light in the light irradiation step, wherein, in the conjunctiva staining step, goblet cells of the ocular conjunctiva are stained with the fluoroquinolone antibiotic; in the light irradiation step, the light source emits single photons; and, in the conjunctiva photographing step, the image pickup unit photographing the ocular conjunctiva is a high-magnification fluorescence microscope or a slit lamp microscope.

公开(公告)号：US20190059737A1

公开(公告)日：2019-02-28

申请号：US15893877

申请日：2018-02-12

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Seunghun LEE ,  Ki Hean Kim ,  Jun Ho Lee ,  Bumju Kim ,  Byung Ho Oh ,  Myoung Joon Kim ,  Kyo Han Anh ,  Hoon Cheol Jang ,  Won Hyuk Jang

IPC: A61B5/00 ,  A61B1/00 ,  A61B1/06 ,  A61B1/313 ,  A61B3/10 ,  A61B5/08 ,  A61B5/20 ,  A61K49/00

Abstract: Disclosed are an imaging method using fluoroquinolone antibiotics and an imaging device for the same, in which biological tissue is stained with Moxifloxacin as one of fluoroquinolone antibiotics, and the stained biological tissue is subjected to fluorescent image-capture through single-photon excitation with either near-ultraviolet or visible wavelength light instead of either a middle-ultraviolet light source or a femtosecond near-infrared laser device, thereby obtaining morphological information of cells in the biological tissue at a high speed without damage. To this end, an imaging method of using fluoroquinolone antibiotics includes: staining cells of the biological tissue with fluoroquinolone antibiotics; illuminating the excitation light from a light source to the biological tissue stained with the fluoroquinolone antibiotics; and capturing an image of the biological tissue through the fluoroquinolone antibiotics based fluorescence caused by the excitation light illuminated to the biological tissue, wherein the excitation light from the light source includes either near-violet or short visible wavelength light for single photon excitation of the fluoroquinolone antibiotics.