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公开(公告)号:US20190233722A1
公开(公告)日:2019-08-01
申请号:US16378541
申请日:2019-04-08
申请人: PROLUME, LTD.
发明人: Bruce Bryan , Hendrik Schmidt , Millard Cull
CPC分类号: C09K11/07 , A61K38/1767 , A61K38/44 , A61K49/0045 , A63J5/02 , C07D487/04 , C09K2211/1007 , C09K2211/1018 , C09K2211/14 , C12N9/0069 , C12Y113/12007
摘要: Calcium reacting photoproteins are a class of self-illuminating proteins that emit light upon contact with calcium. Fluorescent Proteins are small proteins that change the color of light when excited. Fluorescent Proteins and Calcium Activated Photoproteins can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies. The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create unique and novel cinematic, theatrical, stage, movie and musical concert optical effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment.
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公开(公告)号:US11034886B2
公开(公告)日:2021-06-15
申请号:US16378541
申请日:2019-04-08
申请人: PROLUME, LTD.
发明人: Bruce Bryan , Hendrik Schmidt , Millard Cull
IPC分类号: C07D487/22 , C09K11/07 , C12N9/02 , A61K38/17 , A61K38/44 , A63J5/02 , C07D487/04 , A61K49/00
摘要: Novel compounds in the classes of Imidazo[1,2-α]pyrazines and Imidazo[1,2-α]quinoxalines with a methoxy group modification show unique spectral properties when used as a substrate for luciferases and photo-proteins. Methoxy e-Coelenterazine, an Imidazo[1,2-α] quinoxaline shifts the emission wavelength by 80 nm to blue light compared to e-Coelenterazine, using Renilla reniformis luciferase. The novel analogues described here are useful in combination with fluorescent proteins to create BRET (bioluminescent resonance energy transfer) and for other luminescent assays. In addition these novel compounds can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies.
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公开(公告)号:US10253254B2
公开(公告)日:2019-04-09
申请号:US15490714
申请日:2017-04-18
申请人: PROLUME, LTD.
发明人: Bruce Bryan , Hendrik Schmidt , Millard Cull
IPC分类号: C07D487/22 , C09K11/07 , C07D487/04 , C12N9/02 , A61K38/17 , A61K38/44 , A63J5/02 , A61K49/00
摘要: The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment. A base coelenterazine structure is depicted below.
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公开(公告)号:US11965122B2
公开(公告)日:2024-04-23
申请号:US17344927
申请日:2021-06-10
申请人: PROLUME, LTD.
发明人: Bruce Bryan , Hendrik Schmidt , Millard Cull
IPC分类号: C07D487/22 , A61K38/17 , A61K38/44 , A61K49/00 , A63J5/02 , C07D487/04 , C09K11/07 , C12N9/02
CPC分类号: C09K11/07 , A61K38/1767 , A61K38/44 , A61K49/0045 , A63J5/02 , C07D487/04 , C12N9/0069 , C12Y113/12007 , C09K2211/1007 , C09K2211/1018 , C09K2211/14
摘要: Calcium reacting photoproteins are a class of self-illuminating proteins that emit light upon contact with calcium. Fluorescent Proteins are small proteins that change the color of light when excited. Fluorescent Proteins and Calcium Activated Photoproteins can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies. The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create unique and novel cinematic, theatrical, stage, movie and musical concert optical effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment. A generic formula of Coelenterazine as shown.
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公开(公告)号:US20170217969A1
公开(公告)日:2017-08-03
申请号:US15490714
申请日:2017-04-18
申请人: PROLUME, LTD.
发明人: Bruce Bryan , Hendrik Schmidt , Millard Cull
IPC分类号: C07D487/04 , A63J5/02 , C12N9/02 , C09K11/07 , A61K49/00
CPC分类号: C09K11/07 , A61K38/1767 , A61K38/44 , A61K49/0045 , A63J5/02 , C07D487/04 , C09K2211/1007 , C09K2211/1018 , C09K2211/14 , C12N9/0069 , C12Y113/12007
摘要: Calcium reacting photoproteins are a class of self-illuminating proteins that emit light upon contact with calcium. Fluorescent Proteins are small proteins that change the color of light when excited. Fluorescent Proteins and Calcium Activated Photoproteins can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies. The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create unique and novel cinematic, theatrical, stage, movie and musical concert optical effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment.
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公开(公告)号:US09624425B2
公开(公告)日:2017-04-18
申请号:US14424969
申请日:2013-08-30
申请人: PROLUME, LTD.
发明人: Bruce Bryan , Hendrik Schmidt , Millard Cull
IPC分类号: C07D487/02 , C07D487/22 , C09K11/07 , C07D487/04 , C12N9/02 , A61K38/17 , A61K38/44 , A63J5/02
CPC分类号: C09K11/07 , A61K38/1767 , A61K38/44 , A61K49/0045 , A63J5/02 , C07D487/04 , C09K2211/1007 , C09K2211/1018 , C09K2211/14 , C12N9/0069 , C12Y113/12007
摘要: Novel compounds in the classes of Imidazo[1,2-α]pyrazines and Imidazo[1,2-α]quinoxalines with a methoxy group modification show unique spectral properties when used as a substrate for luciferases and photo-proteins. Methoxy e-Coelenterazine an Imidazo[1,2-α] quinoxaline shifts the emission wavelength by 80 nm to blue light compared to e-Coelenterazine, using Renilla reniformis luciferase. The novel analogs described here are useful in combination with fluorescent proteins to create BRET (bioluminescent resonance energy transfer) and for other luminescent assays. In addition these novel compounds can be used in novelty items and for entertaining purposes to create different light emitting colors using the same luciferase or photoprotein.
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公开(公告)号:US20150225642A1
公开(公告)日:2015-08-13
申请号:US14424969
申请日:2013-08-30
申请人: PROLUME, LTD.
发明人: Bruce Bryan , Hendrik Schmidt , Millard Cull
IPC分类号: C09K11/07 , C12N9/02 , C07D487/04
CPC分类号: C09K11/07 , A61K38/1767 , A61K38/44 , A61K49/0045 , A63J5/02 , C07D487/04 , C09K2211/1007 , C09K2211/1018 , C09K2211/14 , C12N9/0069 , C12Y113/12007
摘要: Calcium reacting photoproteins are a class of self-illuminating proteins that emit light upon contact with calcium. Fluorescent Proteins are small proteins that change the color of light when excited. Fluorescent Proteins and Calcium Activated Photoproteins can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies. The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create unique and novel cinematic, theatrical, stage, movie and musical concert optical effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment.
摘要翻译: 钙反应光蛋白是与钙接触时发光的一类自发光蛋白质。 荧光蛋白是当激发时改变光的颜色的小蛋白质。 荧光蛋白和钙激活的光亮蛋白可用于通过直接应用于观众,周围环境,演员或喷洒在新4D电影中的设置来增强,炫耀,惊奇,惊奇,或以其他方式娱乐观众。 该公开内容包括新颖的Coelenterazine化合物和使用方法,包括用于光蛋白的简单递送装置,通过与含有钙的表面接触而通过其发光反应产生独特和新颖的电影,戏剧,舞台,电影和音乐音乐会光学效果。 钙在大多数表面和环境中是普遍存在的; 这是钙的独特性质,这使得这个新的使用光蛋白进行娱乐。
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