公开(公告)号：US20240025965A1

公开(公告)日：2024-01-25

申请号：US18295068

申请日：2023-04-03

申请人： Jai Prakash ,  Universiteit Twente

发明人： Jai Prakash

IPC分类号： C07K14/705 ,  A61K49/00 ,  C07K14/78 ,  C07K7/08 ,  C07K7/06 ,  A61K47/62 ,  A61K47/60 ,  A61P1/16

CPC分类号： C07K14/70546 ,  A61K49/0045 ,  C07K14/78 ,  C07K7/08 ,  C07K7/06 ,  A61K47/62 ,  A61K47/60 ,  A61P1/16 ,  A61K49/0056 ,  A61K38/00

摘要： The disclosure relates to integrin binding peptides, pharmaceutical compositions comprising the peptides and to uses thereof as therapeutic, diagnostic, imaging and targeting agents.

公开(公告)号：US20190233722A1

公开(公告)日：2019-08-01

申请号：US16378541

申请日：2019-04-08

申请人： PROLUME, LTD.

发明人： Bruce Bryan ,  Hendrik Schmidt ,  Millard Cull

IPC分类号： C09K11/07 ,  A63J5/02 ,  A61K38/44 ,  A61K49/00 ,  C12N9/02 ,  C07D487/04 ,  A61K38/17

CPC分类号： C09K11/07 ,  A61K38/1767 ,  A61K38/44 ,  A61K49/0045 ,  A63J5/02 ,  C07D487/04 ,  C09K2211/1007 ,  C09K2211/1018 ,  C09K2211/14 ,  C12N9/0069 ,  C12Y113/12007

摘要： Calcium reacting photoproteins are a class of self-illuminating proteins that emit light upon contact with calcium. Fluorescent Proteins are small proteins that change the color of light when excited. Fluorescent Proteins and Calcium Activated Photoproteins can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies. The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create unique and novel cinematic, theatrical, stage, movie and musical concert optical effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment.

公开(公告)号：US20190015322A1

公开(公告)日：2019-01-17

申请号：US16136950

申请日：2018-09-20

申请人： Joseph E. Kovarik ,  Katherine Rose Kovarik

发明人： Joseph E. Kovarik ,  Katherine Rose Kovarik

IPC分类号： A61K9/00 ,  A61F5/56 ,  A61K35/741 ,  A61K45/06 ,  A61K35/74 ,  A61K31/047 ,  A61K49/00 ,  A61K35/744 ,  A61K9/70

CPC分类号： A61K9/006 ,  A61F5/566 ,  A61K9/7007 ,  A61K31/047 ,  A61K35/74 ,  A61K35/741 ,  A61K35/744 ,  A61K45/06 ,  A61K49/0045 ,  A61K49/0069

摘要： A method and system to prevent sore throat infections in humans includes the administration of an active ingredient to a site on the mucus membranes of the throat of a human that inhibits adherence and promotes desorption of S. pyogenes to soft tissue surfaces, such as the pharyngeal and oral mucosa of a human. A mucoadhesive strip having a surface topography that resists bioadhesion of undesired bacteria that are typically present in a human's mouth is preferably employed and that has one or more encapsulated agents selected from the group consisting of an antibiotic; lactic acid bacteria; S. pyogenes modified by a CRISPR-Cas system to reduce one or more virulence factors; and a breath mint solution.

公开(公告)号：US20180312567A1

公开(公告)日：2018-11-01

申请号：US15770105

申请日：2016-10-21

申请人： Jai Prakash ,  Universiteit Twente

发明人： Jai Prakash

IPC分类号： C07K14/705 ,  A61K47/62 ,  A61K47/60 ,  A61P1/16 ,  A61K49/00

CPC分类号： A61K49/0045 ,  A61K38/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/70546 ,  C07K14/78

摘要： The invention relates to integrin binding peptides, pharmaceutical compositions comprising the peptides and to uses thereof as therapeutic, diagnostic, imaging and targeting agents.

公开(公告)号：US20180028693A1

公开(公告)日：2018-02-01

申请号：US15613104

申请日：2017-06-02

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Anupama LAKSHMANAN ,  Arash FARHADI ,  Suchita P. NETY ,  Raymond W. BOURDEAU ,  Mikhail SHAPIRO

IPC分类号： A61K49/22 ,  A61B8/08

CPC分类号： A61K49/223 ,  A61B8/481 ,  A61B8/5238 ,  A61K49/0002 ,  A61K49/0045 ,  A61K49/0093 ,  A61K49/221 ,  C07K14/195 ,  C07K2319/00

摘要： Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

公开(公告)号：US09757478B2

公开(公告)日：2017-09-12

申请号：US14990279

申请日：2016-01-07

申请人： Promega Corporation

发明人： Brock Binkowski ,  Braeden Butler ,  Lance P. Encell ,  Frank Fan ,  Brad Hook ,  Paul Otto ,  Gediminas Vidugiris ,  Susan Wigdal ,  Kristopher Zimmerman

IPC分类号： A61K49/00 ,  C12N9/02 ,  C12Q1/37 ,  C12Q1/66 ,  A01K67/027

CPC分类号： A61K49/0045 ,  A01K67/0275 ,  A61K49/0013 ,  C12N9/0069 ,  C12Q1/37 ,  C12Q1/66 ,  C12Y113/12007

摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

公开(公告)号：US20170183663A1

公开(公告)日：2017-06-29

申请号：US15395233

申请日：2016-12-30

申请人： STC.UNM

发明人： Anatoliy Markiv ,  Ravi Venkata Durvasula ,  Angray Singh Kang

IPC分类号： C12N15/62 ,  C12N15/63

CPC分类号： C12N15/62 ,  A61K49/0045 ,  A61K49/0058 ,  A61N5/062 ,  C07K14/001 ,  C07K16/18 ,  C07K16/30 ,  C07K16/32 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/60 ,  C12N15/63 ,  C12Q1/04 ,  G01N33/53 ,  G01N33/582

摘要： Embodiments of the present invention provide for the facile generation of a stable recombinant fusion polypeptides with intrinsic fluorescent properties. The recombinant antibodies may be suitable for qualitative and/or quantitative immunofluorescence analysis. Generally, the fluorescent polypeptides include a fluorescent domain comprising a C-terminus and an N-terminus; a first antibody domain covalently linked to the C-terminus; and a second antibody domain covalently linked to the N-terminus.

公开(公告)号：US09670318B2

公开(公告)日：2017-06-06

申请号：US14723505

申请日：2015-05-28

申请人： Miltenyi Biotec GmbH

发明人： Christian Dose

IPC分类号： C08G65/48

CPC分类号： C08G65/48 ,  A61K49/0041 ,  A61K49/0043 ,  A61K49/0045 ,  A61K49/0054 ,  A61K49/0058 ,  C08G65/33306 ,  C08G65/33396 ,  G01N33/582

摘要： The invention is directed to a fluorescent dye according to the general formula I: with C is a core moiety comprising 20 to 200 atoms; S same or different ether residues comprising 1 to 10 carbon atoms; n is an integer ranging from 2 to 500; m is an integer ranging from 0 to 500; x is an integer ranging from 2 to 50; y is an integer ranging from 1 to 50; R same or different residue comprising a reactive group capable of forming a covalent bond with a biomolecule; F same or different fluorophores covalently bound to (S)n. The fluorescent dyes can be conjugated to a biomolecule and used for flow cytometry and/or by fluorescence microscopy.

公开(公告)号：US20170152295A1

公开(公告)日：2017-06-01

申请号：US15317931

申请日：2015-06-08

申请人： JAPAN SCIENCE AND TECHNOLOGY AGENCY

发明人： Haruhiko BITO ,  Masatoshi INOUE ,  Atsuya TAKEUCHI ,  Junichi NAKAI ,  Masamichi OHKURA

IPC分类号： C07K14/47 ,  C12Q1/68 ,  A61K49/00 ,  C12N9/12

CPC分类号： C07K14/47 ,  A01K67/027 ,  A61K49/0045 ,  C07K14/435 ,  C07K19/00 ,  C07K2319/60 ,  C12N5/10 ,  C12N9/12 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/66 ,  C12Q1/6897 ,  C12Y207/11017

摘要： As a calcium indicator protein having an excellent fluorescent characteristic and calcium reactivity, there is provided DNA in which one of a nucleotide sequence derivative of a calmodulin-binding sequence (ckkap sequence) of calcium/calmodulin-dependent protein kinase kinase and a nucleotide sequence encoding a calcium-binding sequence (CaM sequence) of calmodulin is linked to a 5′ end of a nucleotide sequence encoding a fluorescent protein, and the other nucleotide sequence is linked to a 3′ end of the nucleotide sequence encoding the fluorescent protein. The calcium indicator protein encoded by this DNA, which based on the derivative of the ckkap sequence as a binding domain for the calcium-bound CaM sequence, exhibits a fluorescent characteristic and calcium reactivity superior to those of conventional calcium indicator proteins.

公开(公告)号：US09644007B2

公开(公告)日：2017-05-09

申请号：US14974483

申请日：2015-12-18

申请人： Howard Hughes Medical Institute

发明人： Douglas S. Kim ,  Loren L. Looger ,  Eric R. Schreiter ,  Karel Svoboda

IPC分类号： C07K14/00 ,  C07K14/435 ,  C07K14/46 ,  C07K14/47 ,  G01N33/53 ,  G01N33/567 ,  C12N5/06 ,  C12N5/10 ,  A61K38/00 ,  G01N33/50 ,  A61K49/00 ,  A01K67/027 ,  G01N33/58

CPC分类号： C07K14/00 ,  A01K67/0275 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2227/40 ,  A01K2227/703 ,  A01K2227/706 ,  A01K2267/0393 ,  A61K49/0045 ,  C07K14/001 ,  C07K14/4728 ,  C07K2319/095 ,  C07K2319/20 ,  C12N2750/14143 ,  G01N33/5041 ,  G01N33/582 ,  G01N2333/4727

摘要： Protein indicators useful for calcium imaging, in particular, red genetically-encoded calcium indicators (GECIs) disclosed herein rival best-of-class green GECIs in terms of sensitivity for detecting neural activity, and can be monitored in vivo. The presently-disclosed subject matter further includes a method of monitoring cell activity comprising stimulating a cell comprising a red GECI polypeptide; and detecting fluorescence emitted by the cell.