-
1.发明授权On-chip hybridization coupled with ITP based purification for fast sequence specific identification 有权片上杂交与基于ITP的纯化相结合，用于快速序列特异性鉴定公开(公告)号：US08524061B2
公开(公告)日：2013-09-03
申请号：US13373773
申请日：2011-11-29
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44726 , C07K1/26 , C12Q1/68 , C12Q1/6816 , G01N33/6803 , C12Q2525/207 , C12Q2525/301 , C12Q2565/125 , C12Q2565/629
摘要： Isotachophoresis (ITP) can be employed to simultaneously focus the target and ligand of an assay into the same ITP focus zone. The target and ligand can bind to each other in the ITP focus zone, and then the resulting bound complex can be detected (e.g., by fluorescence). The sensitivity of this approach can be greatly increased by the enhanced concentration of both target and ligand that ITP provides in the focus zone. Since ITP can be performed quickly, the resulting assay is both rapid and sensitive. Markers of bacterial urinary tract infections have been experimentally detected at clinically relevant concentrations with this approach. MicroRNA sequences have also been profiled with this approach, which is clinically relevant because MicroRNA is expected to provide useful markers for disease. In one experiment, miR-122 in human kidney and liver was detected and quantified.
摘要翻译： 可以使用等速电泳（ITP）来同时将测定的靶和配体聚焦到相同的ITP聚焦区域中。 靶和配体可以在ITP聚焦区域中彼此结合，然后可以检测所得到的结合复合物（例如通过荧光）。 通过ITP在对焦区域提供的靶和配体的增强浓度，可以大大提高该方法的灵敏度。 由于ITP可以快速进行，因此得到的测定既快速又敏感。 通过这种方法已经在临床相关浓度上实验检测到细菌性尿路感染的标记。 MicroRNA序列也已经用这种方法进行了描述，这是临床上相关的，因为预期MicroRNA可以为疾病提供有用的标记。 在一个实验中，检测并定量人肾脏和肝脏中的miR-122。
-
2.发明申请On-chip hybridization coupled with ITP based purification for fast sequence specific identification 有权片上杂交与基于ITP的纯化相结合，用于快速序列特异性鉴定公开(公告)号：US20120160689A1
公开(公告)日：2012-06-28
申请号：US13373773
申请日：2011-11-29
IPC分类号： G01N27/447
CPC分类号： G01N27/44726 , C07K1/26 , C12Q1/68 , C12Q1/6816 , G01N33/6803 , C12Q2525/207 , C12Q2525/301 , C12Q2565/125 , C12Q2565/629
摘要： Isotachophoresis (ITP) can be employed to simultaneously focus the target and ligand of an assay into the same ITP focus zone. The target and ligand can bind to each other in the ITP focus zone, and then the resulting bound complex can be detected (e.g., by fluorescence). The sensitivity of this approach can be greatly increased by the enhanced concentration of both target and ligand that ITP provides in the focus zone. Since ITP can be performed quickly, the resulting assay is both rapid and sensitive. Markers of bacterial urinary tract infections have been experimentally detected at clinically relevant concentrations with this approach. MicroRNA sequences have also been profiled with this approach, which is clinically relevant because MicroRNA is expected to provide useful markers for disease. In one experiment, miR-122 in human kidney and liver was detected and quantified.
摘要翻译： 可以使用等速电泳（ITP）来同时将测定的靶和配体聚焦到相同的ITP聚焦区域中。 靶和配体可以在ITP聚焦区域中彼此结合，然后可以检测所得到的结合复合物（例如通过荧光）。 通过ITP在对焦区域提供的靶和配体的增强浓度，可以大大提高该方法的灵敏度。 由于ITP可以快速进行，因此得到的测定既快速又敏感。 通过这种方法已经在临床相关浓度上实验检测到细菌性尿路感染的标记。 MicroRNA序列也已经用这种方法进行了描述，这是临床上相关的，因为预期MicroRNA可以为疾病提供有用的标记。 在一个实验中，检测并定量人肾脏和肝脏中的miR-122。
-
搜索结果
2 条记录 (耗时 0.02 秒)
